Head and neck squamous cell carcinoma lymphatic spread and survival: Relevance of vascular endothelial growth factor family for tumor evaluation

Sousa, Elen Alves de; Lourenço, Sílvia Vanessa; Moraes, Flávia Paiva Prudente de; Vartanian, José Guilherme; Gonçalves-Filho, João; Kowalski, Luiz Paulo; Soares, Fernando Augusto; Coutinho‐Camillo, Cláudia Malheiros

doi:10.1002/hed.23765

Cited by 11 publications

(9 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At present, studies have found that tobacco and alcohol are important factors in inducing HNSCC (Jethwa & Khariwala, 2017;Kawakita & Matsuo, 2017). The incidence of HNSCC is also relatively high in the world, which not only directly affects patients' breathing, eating and language functions, but also causes rapid growth of neck lymph node metastasis with the help of abundant blood supply and lymphatic refluence (De Sousa et al, 2015). The main clinical treatment methods for HNSCC include surgery, radiotherapy and chemotherapy (Cramer et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

The m6A reader protein YTHDC2 is a potential biomarker and associated with immune infiltration in head and neck squamous cell carcinoma

Zheng

Wang

et al. 2020

PeerJ

View full text Add to dashboard Cite

Background Increasing evidence has shown that N6-methyladenosine (m6A) RNA methylation regulators have important biological functions in human cancers. However, there are few studies on the value of m6A reader protein YTHDC2 in the diagnosis and tumor-infiltrating of head and neck squamous cell carcinoma (HNSCC). Therefore, it is important to understand the potential clinical value of YTHDC2 in the prognosis and immune infiltration of HNSCC. Methods In this study, gene expression profiles and the corresponding clinical information of 270 HNSCC patients were downloaded from the Gene Expression Omnibus (GEO) database. The gene co-expression network was established to verify whether YTHDC2 was related to the prognosis of HNSCC and verified again in the public database. The correlations between YTHDC2 and immune infiltration was investigated via Tumor Immune Estimation Resource (TIMER) and Gene Expression Profiling Interactive Analysis (GEPIA). Results The results showed that YTHDC2 appeared in the blue module related to survival time and survival state and had a close correlation with the prognosis and immune infiltration level of HNSCC in public database. Patients with low expression of YTHDC2 had poor overall survival (OS) and recurrence-free survival (RFS) than those with high expression. In addition, the expression of YTHDC2 was positively correlated with the level of CD4+ T cell subpopulations infiltration in HNSCC. Conclusions Through this study, we found that YTHDC2 is a tumor suppressor gene with high expression in normal tissues and low expression in tumor tissues. In addition, YTHDC2 is correlated with the immune infiltrating levels of B cells, CD8+ T cells, CD4+ T cells, neutrophils, and dendritic cells in HNSCC, which may become a potential marker for prognosis and immune infiltration of HNSCC.

show abstract

Section: Introductionmentioning

confidence: 99%

The m6A reader protein YTHDC2 is a potential biomarker and associated with immune infiltration in head and neck squamous cell carcinoma

Zheng

Wang

et al. 2020

PeerJ

View full text Add to dashboard Cite

show abstract

“…20 Although it is not yet clear which of peritumoral 21 or intratumoral lymphatic vessel density [22][23][24][25] is more relevant for the development of lymph node metastasis, a great number of studies have shown a significant association between lymphatic vessel density and lymph node metastasis in HNSCC, mostly using antibodies against podoplanin or the anti-hyaluronan receptor LYVE-1. 29 In this study, we used a specific antibody against the transcription factor Prospero homeobox protein 1 (PROX1) as an indicator of lymphatic vessel density. 29 In this study, we used a specific antibody against the transcription factor Prospero homeobox protein 1 (PROX1) as an indicator of lymphatic vessel density.…”

Section: Introductionmentioning

confidence: 99%

“…23,[25][26][27][28] However, the use of disparate assessment methods across studies make it difficult to draw definitive conclusions on the use of lymphatic vessel density as a predictor of lymph node metastasis. 29 In this study, we used a specific antibody against the transcription factor Prospero homeobox protein 1 (PROX1) as an indicator of lymphatic vessel density. Its specific lymphatic endothelial cells nuclear staining 30 allows for precise quantification of lymphatic vessels without the risk of misinterpretations from morphological pitfalls and/or unspecific staining.…”

Section: Introductionmentioning

confidence: 99%

Prediction of occult lymph node metastasis in squamous cell carcinoma of the oral cavity and the oropharynx using peritumoral Prospero homeobox protein 1 lymphatic nuclear quantification

et al. 2016

View full text Add to dashboard Cite

show abstract

“…It has been demonstrated that high MVD is associated with both metastasis and poor prognosis in patients with head and neck cancers . Lymphatic vasculature has also been shown to play a role, with high LVD being associated with poor prognosis in gastric, non‐small‐cell lung, and head and neck cancers . Many of these studies have distinguished between peritumoral and intratumoral vessel densities.…”

Section: Discussionmentioning

confidence: 99%

“…9,22 Lymphatic vasculature has also been shown to play a role, with high LVD being associated with poor prognosis in gastric, non-small-cell lung, and head and neck cancers. 10,11,[23][24][25][26][27][28] Many of these studies have distinguished between peritumoral and intratumoral vessel densities. In HNSCC, high intratumoral LVD has been reported to be associated with higher risk of local recurrence, 10 cervical nodal metastases, 11,29 and worse overall survival.…”

Section: Discussionmentioning

confidence: 99%

Prognostic implications of peritumoral vasculature in head and neck cancer

et al. 2018

View full text Add to dashboard Cite

BackgroundThere is conflicting evidence regarding the role of peritumoral lymphatic vessel density (LVD) and blood microvessel density (MVD) in the metastasis and prognosis of head and neck squamous cell carcinoma (HNSCC). Existing studies are limited to one or two head and neck subsites and/or small sample sizes. A larger study incorporating multiple sub‐sites is needed to address the role of peritumoral LVD and MVD in HNSCC metastasis and prognosis.MethodsTissue samples from 200 HNSCC cases were stained simultaneously using immunohistochemistry (IHC) for markers of peritumoral LVD (lymphatic vessel marker D240) and MVD (blood vessel marker CD31). Of the stained slides, 166 and 167 were evaluable for LVD and MVD, respectively. The results were then correlated with clinicopathologic features and patient outcomes.ResultsPatients with metastatic disease were more likely to have high peritumoral MVD. Through multivariable analyses, MVD was not significantly related to DFS and OS, while low LVD was related to higher risk of disease progression and poor survival.ConclusionsPeritumoral MVD was found to be positively associated with metastasis, while LVD was found to be inversely related to both metastasis and progression of HNSCC. These findings may suggest a prognostic role of both peritumoral LVD and MVD in patients with HNSCC.

show abstract

Head and neck squamous cell carcinoma lymphatic spread and survival: Relevance of vascular endothelial growth factor family for tumor evaluation

Abstract: Although lymphatic spread is a significant event in the progression of HNSCC, the expression of VEGF-C, VEGF-D, and VEGFR3 does not correlate with clinicopathological characteristics, suggesting that other signaling pathways mediate lymphangiogenesis in HNSCC.

Cited by 11 publications

References 48 publications

The m6A reader protein YTHDC2 is a potential biomarker and associated with immune infiltration in head and neck squamous cell carcinoma

The m6A reader protein YTHDC2 is a potential biomarker and associated with immune infiltration in head and neck squamous cell carcinoma

Prediction of occult lymph node metastasis in squamous cell carcinoma of the oral cavity and the oropharynx using peritumoral Prospero homeobox protein 1 lymphatic nuclear quantification

Prognostic implications of peritumoral vasculature in head and neck cancer

Contact Info

Product

Resources

About