Head kidney neutrophils of carp (Cyprinus carpio L.) are functionally modulated by the haemoflagellateTrypanoplasma borreli

Scharsack, Jörn P.; Steinhagen, Dieter; Kleczka, C.; Schmidt, Jörn O.; Körting, W.; Michael, R. Dinakaran; Leibold, W.; Schuberth, Hans‐Joachim

doi:10.1006/fsim.2002.0447

Cited by 21 publications

(19 citation statements)

References 26 publications

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“…data). Also, Scharsack et al (2003), using flow cytometry, found no evidence for phagocytosis of fluorochrome-labelled parasites. In addition, goldfish macrophages do not seem to phagocytose T. carassii (M. Belosevic pers.…”

Section: Discussionmentioning

confidence: 93%

Minor effect of depletion of resident macrophages from peritoneal cavity on resistance of common carp Cyprinus carpio to blood flagellates

et al. 2003

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Carp Cyprinus carpio macrophages were depleted by intraperitoneal (i.p.) injection of clodronate-liposomes for the in vivo study of the effect of macrophage depletion on the resistance of carp to infection with blood flagellate parasites. Clodronate released inside the cell induces apoptosis of (murine) macrophages. Following i.p. injection of carp with liposomes alone, but not with Trypanoplasma borreli, neutrophilic granulocytes rapidly migrated from the head kidney to the peritoneal cavity. The majority of liposomes in the peritoneal cavity were not taken up by newly arrived neutrophilic granulocytes, however, but by resident macrophages. After 2 i.p. injections of clodronate-liposomes, the percentage of macrophages present in the peritoneal cavity was significantly reduced, as evaluated by flow cytometry. Macrophage-depleted carp that were infected i.p. with T. borreli suffered from high mortality. However, these fish did not show lethal parasitaemia but did show clear bacteraemia. Macrophage-depleted carp that were infected i.p. with Trypanosoma carassii showed a minor increase in parasitaemia. In addition, macrophage-depleted carp, immune to T. borreli as a result of having survived a prior infection, remained immune to i.p. reinfection with T. borreli. Succesful depletion of peritoneal macrophages seemed to have a minor effect on the resistance of carp against blood flagellates. However, carp macrophages are essential as a first line of defence against (bacterial) infection. KEY WORDS: Cyprinus carpio · Macrophages · Clodronate · Trypanoplasma borreli · Trypanosoma carassiiResale or republication not permitted without written consent of the publisher

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Section: Discussionmentioning

confidence: 93%

Minor effect of depletion of resident macrophages from peritoneal cavity on resistance of common carp Cyprinus carpio to blood flagellates

et al. 2003

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“…It could be that the granulocytes, which are expected to have iNOS activity [22], affect the NO production or arginase activity measured.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies showed different responses of carp against the two parasite species with regard to nitrite production [11,22]. T. borreli was shown to induce a high amount of nitrite production by head kidney leukocytes, both in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 98%

Differential macrophage polarisation during parasitic infections in common carp (Cyprinus carpio L.)

Joerink

Forlenza

Ribeiro

et al. 2006

Fish & Shellfish Immunology

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“…The neutrophil was characterized by ungranulated cytoplasm (Kobayashi et al 2006) and a smaller, more compact nucleus. Its size decreased during ageing (Scharsack et al 2003), and the nucleus could become band (Fig. 2.9) and then segmented (Fig.…”

Section: Erythroid Cellsmentioning

confidence: 99%

Haematopoiesis in the head kidney of common carp (Cyprinus carpio L.): a morphological study

Kondera¹

2010

Fish Physiol Biochem

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The morphogenesis of head kidney haematopoietic cells in common carp has been shown to be very similar to that of higher vertebrates. The erythropoietic series consisted of the erythroblasts (basophilic, polychromatic and orthochromic), young erythrocyte and erythrocyte. The morphological changes that occur during maturation process are reduction in size and further increase at mature stage, gradual chromatin condensation and the increase in haemoglobin content. Maturation stages of neutrophils and basophils encompassed the young progranulocyte, progranulocyte and metagranulocyte. The early blast cells, basophilic lineage and lymphocytes were regularly present in kidney. In the monocytoid series in kidneys, promonocytes and mature monocytes were observed. Precursor stages of the eosinophile and thrombocyte were not discernible (although mature cells were observed). Lymphoid cells were the most abundant, followed by granuloid, thrombocyte, erythroid, blast and monocytoid cells. The neutrophilic progranulocyte was the most frequent granuloid cell. The lymphocyte was the most frequent cell in the kidney and the most numerous of the lymphoid lineage.

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Head kidney neutrophils of carp (Cyprinus carpio L.) are functionally modulated by the haemoflagellateTrypanoplasma borreli

Cited by 21 publications

References 26 publications

Minor effect of depletion of resident macrophages from peritoneal cavity on resistance of common carp Cyprinus carpio to blood flagellates

Minor effect of depletion of resident macrophages from peritoneal cavity on resistance of common carp Cyprinus carpio to blood flagellates

Differential macrophage polarisation during parasitic infections in common carp (Cyprinus carpio L.)

Haematopoiesis in the head kidney of common carp (Cyprinus carpio L.): a morphological study

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