Head Space Solid Phase Micro-Extraction (HS - SPME) of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636)

Valduga, Eunice; Alexsandra, Valerio; Treichel, Helen; Filho, Irajá do Nascimento; Fúrigo, Agenor; Luccio, Marco Di

doi:10.1590/s0101-20612010000400023

Cited by 7 publications

(7 citation statements)

References 33 publications

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“…Within these systems, various research groups have refi ned them by altering the conditions of stirring, heating (e.g., 25-80 °C) and/or collection timing (e.g., 10-30 min). In addition, saturated solutions of various salts (e.g., KCl, NaCl, Na 2 SO 4 , NaH 2 PO 4 ) have been applied to improve the quantity and quality of compounds discovered by GS/ MS analysis (Valduga et al 2010 ). All studies have shown that the mechanism of mVOCs production is extremely specifi c, depending on not only the microbial strain but also the culture medium and analysis and the analytical techniques used.…”

Section: Collection Of Mvocsmentioning

confidence: 99%

“…Therefore it is suitable for analysis of VOCs present in fermented broths and in samples with low analyte concentration. The nondestructive application of a thin polymeric fi lm immobilized on a fused silica core allows evaluation of samples under varying experimental conditions and can be used to resolve small inorganic or organic gaseous molecules such as CO, CO 2 , NH 3 , HCN, and ethylene Valduga et al 2010 ). Head space extractions of mVOCs are typically performed on a magnetic stirrer.…”

Section: Head Space-solid Phase Microextraction (Hs-spme)mentioning

confidence: 99%

“…The fi bers are then desorbed at 210 °C in the injection port of a GC-MS. The fi bers are cleaned up leaving them in the injection port for 10 min after each run (Farag et al 2006 ;Valduga et al 2010 ;Lee et al 2012 ).…”

Section: Head Space-solid Phase Microextraction (Hs-spme)mentioning

confidence: 99%

“…The effect of mVOCs was much less notable on the relatively nutrient-poor media MS and Angle (Blom et al 2011 ). Others researchers examined mVOCs production on culture media supplemented with mannitol or glucose, and found that the mVOCs composition and quantities of various compounds varied depending on the carbon source Valduga et al 2010 ). In conclusion, mVOC production is closely related to culture media composition.…”

Section: Culture Mediummentioning

confidence: 99%

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Production of Volatile Organic Compounds in PGPR

Santoro

Cappellari

Giordano

et al. 2015

Handbook for Azospirillum

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Bacteria affect plant growth through several mechanisms. A recently described mechanism involves the production of microbial volatile organic compounds (mVOCs), which are gaseous molecules capable of interacting with plants in the soil environment. mVOCs may promote plant growth directly, through induced resistance systemic (ISR), or indirectly through suppression of phytopathogens (biocontrol). In this chapter, we describe several experimental designs for evaluation of mVOCs effects on plant growth, ISR or biocontrol mechanisms; potential problems with the methodologies and possible solutions. To date, relatively few mVOCs have been identifi ed and their effects on plant growth characterized. Generally, the effect observed on a particular plant-bacterium interaction was attributed to the pool of mVOCs produced, like an evidence of synergism among chemical compounds.

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Section: Collection Of Mvocsmentioning

confidence: 99%

Section: Head Space-solid Phase Microextraction (Hs-spme)mentioning

confidence: 99%

Section: Head Space-solid Phase Microextraction (Hs-spme)mentioning

confidence: 99%

Section: Culture Mediummentioning

confidence: 99%

See 2 more Smart Citations

Production of Volatile Organic Compounds in PGPR

Santoro

Cappellari

Giordano

et al. 2015

Handbook for Azospirillum

View full text Add to dashboard Cite

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“…The only non-automated parts of the method are the sample preparation and the data analysis. Aroma compounds in wine are often identified and quantified with SPME (De Calle García et al 1998, Vas et al 1998, Bonino et al 2003, Díaz-Maroto et al 2004, Siebert et al 2005, Risticevic et al 2010, Valduga et al 2010. Pawliszyn (Arthur andPawliszyn 1990, Spietelun et al 2010) designed SPME to allow for automation of the similar technique solid-phase extraction (SPE).…”

Section: Introductionmentioning

confidence: 99%

Comparative study on the sensitivity of solid-phase microextraction fibre coatings for the analysis of fermentation bouquet compounds

Haggerty

Bowyer

Jiranek

et al. 2014

Australian Journal of Grape and Wine Research

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Background and Aims: Solid-phase microextraction (SPME) is a popular tool for the analysis of volatile compounds of wines. Given the constant introduction of new and improved SPME fibres, an updated study on the appropriateness of various fibre coatings for the extraction and quantification of key aroma compounds in wines is crucial for wine analysis research. This study aims to identify the optimal fibre for the analysis of the most important 'fermentation bouquet' aroma compounds found in young white wines through the application of a novel scoring system. Methods and Results: Fibre efficiency was determined by coefficients of determination from standard curves in 10% (v/v) ethanol solution. The concentration of the samples within the standard curves was established at twice the average concentration reported in wine as the maximum and then dilutions to 40, 2, 0.8 and 0.04% of this maximum concentration. A novel scoring system provided a thorough analysis of the benefits and disadvantages of the five most common, commercially available fibres recommended for aroma compound analysis and quantification for a library of 38 fermentation bouquet aroma compounds commonly found in finished white wines. Conclusions: Based on our findings, it is recommended that studies of the fermentation bouquet of wines employ either the 65 μm divinylbenzene/polydimethylsiloxane or the 100 μm polydimethylsiloxane fibres for overall extraction, based on peak symmetry, coefficients of determination of the linear curve and sensitivity. Significance of the Study: This study details a novel scoring system that allows determination of the best fibre coating for the analysis of compounds contributing to the fermentation bouquet of white wines by headspace SPME coupled with gas chromatography-mass spectrometry.

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Qualitative and Untargeted Volatilome Fingerprinting of Aspergillus sp. and Bulbithecium sp. by HS‐SPME‐GCMS and Functional Interactions

Mancheary John,

Kandula,

Cheekatla

et al. 2024

Journal of Basic Microbiology

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Research on fungal volatile organic compounds (VOCs) has increased worldwide in the last 10 years, but marine fungal volatilomes remain underexplored. Similarly, the hormone‐signaling pathways, agronomic significance, and biocontrol potential of VOCs in plant‐associated fungi make the area of research extremely promising. In the current investigation, VOCs of the isolates—Aspergillus sp. GSBT S13 and GSBT S14 from marine sediments, and Bulbithecium sp. GSBT E3 from Eucalyptus foliage were extracted using Head Space solid phase microextraction, followed by gas chromatography‐mass spectrometry, identification, statistical analyses, and prediction of functions by KEGG COMPOUND and STITCH 5.0 databases. The significance of this research is fingerprinting VOCs of the isolates from distinct origins, identification of compounds using three libraries (NIST02, NIST14, and W9N11), and using bioinformatic tools to perform functional analysis. The most important findings include the identification of previously unreported compounds in fungi—1‐methoxy naphthalene, diethyl phthalate, pentadecane, pristane, and nonanal; the prediction of the involvement of small molecules in the degradation of aromatic compound pathways and activation, inhibition, binding, and catalysis of metabolites with predicted protein partners. This study has ample opportunity to validate the findings and understand the mechanism or mode of action, the interspecies interactions, and the role of the metabolites in geochemical cycles.

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Head Space Solid Phase Micro-Extraction (HS - SPME) of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636)

Cited by 7 publications

References 33 publications

Production of Volatile Organic Compounds in PGPR

Production of Volatile Organic Compounds in PGPR

Comparative study on the sensitivity of solid-phase microextraction fibre coatings for the analysis of fermentation bouquet compounds

Qualitative and Untargeted Volatilome Fingerprinting of Aspergillus sp. and Bulbithecium sp. by HS‐SPME‐GCMS and Functional Interactions

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