Head-to-head comparison of Enzyme Linked Immunosorbent Assay (ELISA) and Enhanced Chemiluminescence Immunoassay (ECLIA) for the detection of Transfusion Transmitted Disease (TTD) Markers; HIV, HCV and HBV in blood donors, in India

Enzyme-linked immunosorbent assay (ELISA) technique measures antigens, antibodies, and protein reactions in biological samples by enzymatic reactions. The chemiluminescence immunoassay (CLIA) technique determines sample concentrations based on the intensity of the light emitted by a chemical and biological reaction. This review provides an overview to understand the ELSIA and CLIA methods with their types and comparison. ELISA and CLIA methods were compared based on previous literature studies. In conclusion, CLIA is found highly sensitive, specific, and rapid, as compared to ELISA, but CLIA is an expensive method as compared to ELISA.

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“…Both tests were 100% sensitive and specific [10] Diagnostic accuracy of serological tests for COVID-19: Systematic review and meta-analysis.…”

Section: Research Title Results Referencesmentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay versus Chemiluminescent Immunoassay: A General Overview

Khan

Shah

Salman

et al. 2023

GJMPBU

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“…HBV infection can be detected either by DNA isolation and amplification by PCR or immunoassays [28]. PCR is more sensitive, but it is more difficult and expensive [28]- [30]. On the other hand, immunoassay such as ELISA is easier and faster to detect a higher number of samples [31], [32].…”

Section: Resultsmentioning

confidence: 99%

Establishment of anti-HBcAg Monoclonal Antibodies for Sandwich ELISA Application by Iliac Method Utilizing Incomplete Adjuvant

Septisetyani¹,

Herlina²,

Kusumawati³

et al. 2021

International Journal on Advanced Science, Engineering and Information Technology

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The prevalence of hepatitis B virus (HBV) infection in Indonesia was moderate in 2013. This makes an appropriate action has to be taken immediately. In an attempt to evaluate our candidate HBV vaccine efficacy, we generated monoclonal antibodies specific to HBcAg for sandwich ELISA application for research purposes. Monoclonal antibodies are generally developed using hybridoma technology by isolating B cells from spleen or lymph nodes followed by fusion with myeloma cells. This study generated hybridoma by modifying the iliac lymph node method and used incomplete Freund's adjuvant to emulsify the antigen. For this purpose, an elevenweek-old BALB/c mouse was immunized with a single shot recombinant HBcAg water in oil emulsion intramuscularly at the mouse tail base. After one month, besides enlargement of the medial iliac lymph nodes, we also found that the sub-iliac lymph nodes were enlarged. In two different fusion attempts, the single B cells were then isolated from each lymph node and fused with SP2/0-Ag14 mouse myeloma cells. Hybridoma cells derived from both lymph nodes were screened by ELISA coated with HBcAg peptide. As a result, we obtained two positive wells of hybridoma polyclones from each medial iliac and sub-iliac-derived B lymphocyte. After monoclonalization, we obtained candidate anti-HBcAg monoclonal antibodies as either capture or detection antibodies. Furthermore, we successfully retrieved hybridoma clones 9.3, 9.4, and 9.5, which could produce monoclonal antibodies for sandwich ELISA application.

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“…Moreover, ICT is a screening test and is dependent upon further testing for disease confirmation. On the contrary, chemiluminescence has high sensitivity and specificity than other traditional methods of detection and has been documented to be comparable to 8 or even superior to ELISA in terms of sensitivity 7 .…”

Section: Discussionmentioning

confidence: 99%

Frequency of HIV Positivity in Healthy Blood Donors presenting to a Tertiary Care Hospital

Munir¹,

Asgher²,

Ghazanfar³

et al. 2022

PJMHS

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Background: HIV has been under serious consideration by the World Health Organization as well as the Federal government and Provincial health departments. Strict measures have been taken and protocols implemented to curtail the spread of HIV along with Hepatitis B and Hepatitis C. Data from privately-run blood banks have not been reflected in the last 15 years. Aim: To identify current trends of HIV positivity in Lahore Methods: This prospective cross-sectional study was conducted at the Department of Pathology & Laboratory, Doctors Hospital & Medical Centre, Lahore from August 2021 to January 2022. After fulfilling the selection criteria 3160 non-remunerated blood donors selected through non-probability consecutive sampling, were enrolled. Anti-HIV test was performed using chemiluminescence. Results: 3160 voluntary blood donors were investigated. All subjects were males with mean age 29.7 years ranging from 21 years to 46 years. 2 candidates for blood donation (0.06%) were tested positive for HIV. Conclusion: This study reflects stagnation in HIV control as depicted by the frequency of its positivity. There is need to enhance awareness programs in controlling the spread of blood borne diseases especially HIV. Keywords: HIV, blood donor, blood borne diseases, blood transfusion

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Head-to-head comparison of Enzyme Linked Immunosorbent Assay (ELISA) and Enhanced Chemiluminescence Immunoassay (ECLIA) for the detection of Transfusion Transmitted Disease (TTD) Markers; HIV, HCV and HBV in blood donors, in India

Cited by 16 publications

References 14 publications

Enzyme-Linked Immunosorbent Assay versus Chemiluminescent Immunoassay: A General Overview

Enzyme-Linked Immunosorbent Assay versus Chemiluminescent Immunoassay: A General Overview

Establishment of anti-HBcAg Monoclonal Antibodies for Sandwich ELISA Application by Iliac Method Utilizing Incomplete Adjuvant

Frequency of HIV Positivity in Healthy Blood Donors presenting to a Tertiary Care Hospital

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