Head‐to‐head comparison of genotyping of human papillomavirus by real‐time multiplex PCR assay using type‐specific primers and SPF10‐PCR‐based line probe assay

Abstract

The SPF10-polymerase chain reaction (PCR)-based line probe assay (LiPA-25) with high analytical sensitivity and specificity for human papillomavirus (HPV) genotyping in clinical samples has been widely used in vaccine and epidemiologic studies. A realtime multiplex PCR assay using type-specific primers (Hybribio-23) with low workload and cost has been developed recently. The study aimed to compare the

Evaluation of an E6/E7 PCR‐capillary electrophoresis fragment analysis in the genotyping of human papillomavirus in archival FFPE samples of oropharyngeal cancer

“…As for HPV58, 1 positive case was confirmed with SureX, but none with LiPA‐25. Previous studies with cervical samples have also found LiPA‐25 was less sensitive to HPV58 than other assays 29–31 …”

“…Previous studies with cervical samples have also found LiPA-25 was less sensitive to HPV58 than other assays. [29][30][31] T A B L E 5 Concordance of the human papillomaviruses results between LiPA-25, SureX, and p16 ink4a expression. HPV DNA and p16 ink4a detection are established markers for HPV-related OPSCC.…”

“…Genotyping results of LiPA-25 and SureX were divided into 3 groups: concordant (100% identical), compatible (at least one genotype of multiple infections was detected by both assays), and discordant. 28,29 The level of agreement was determined using Cohen's Kappa…”

“…Genotyping results of LiPA‐25 and SureX were divided into 3 groups: concordant (100% identical), compatible (at least one genotype of multiple infections was detected by both assays), and discordant 28,29 . The level of agreement was determined using Cohen's Kappa statistics, among which two‐tailed McNemar's test was used for mutual comparisons of the positivity rates of LiPA‐25, SureX and p16 ink4a expression.…”

Ranking the Attribution of High-Risk Genotypes among Women with Cervical Precancers and Cancers: A Cross-Sectional Study in Ningbo, China

“…(18) We additionally used the SureX HPV 25X Genotyping Kit (Health Gene Tech, Ningbo, China) on the remaining DNA extracts from samples with negative genotyping results, due to the inferior sensitivity of the SPF 10 -PCR-DEIA-LiPA 25 version 1 detection system for certain HR-HPV genotypes. (19,20) The SureX HPV 25X Genotyping Kit is a multiplex PCR test that targets the E6/E7 DNA regions of the HPV genome. This test can differentiate HPV6, 11,16,18,26,31,33,35,39,42,43,44,45,51,52,53,56,58,59, 66, 68, 73, 81, 82 and 83.…”