Headspace analysis of volatile metabolites of Pseudomonas aeruginosa and related species by gas chromatography-mass spectrometry

Labows, John N.; McGinley, Kenneth J.; Webster, Guy F.; Leyden, James J.

doi:10.1128/jcm.12.4.521-526.1980

Cited by 153 publications

(100 citation statements)

References 35 publications

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“…n = Total number of samples assayed. ginosa grown in trypticase soy broth media (Labows et al 1980;Zechman and Labows 1985). Notably 2-nonanone was also present in the headspace gases of sputa taken from the no pathogen group, suggesting that it may not be a simplistic presence ⁄ absence marker that denotes Ps.…”

Section: Discussionmentioning

confidence: 99%

Volatile biomarkers of Pseudomonas aeruginosa in cystic fibrosis and noncystic fibrosis bronchiectasis

Savelev

Perry

Bourke

et al. 2011

Letters in Applied Microbiology

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Aims: The purpose of this study was to determine whether volatile organic compounds specific to Pseudomonas aeruginosa could be detected in clinical sputum specimens. Methods and Results: Patients were recruited from specialist bronchiectasis and cystic fibrosis clinics. The gold standard for diagnosing Ps. aeruginosa infection was a positive sputum culture. About 72 sputum headspace samples taken from patients at risk of or known to have prior Ps. aeruginosa infection were analysed by solid phase micro‐extraction mass spectrometry. 2‐nonanone was a marker in Ps. aeruginosa in sputum headspace gas with sensitivity of 72% and specificity of 88%. A combination of volatile compounds, a sputum library of 17 compounds with 2‐nonanone, increased sensitivity in the detection of Ps. aeruginosa to 91% with specificity of 88%. Conclusions: In contrast to the 48‐hour turnaround for classical microbiological culture, these results were available within 1–2 h. These data demonstrate the potential for rapid and accurate diagnosis of Ps. aeruginosa infection from sputum samples. Significance and impact of the study: 2‐Nonanone is a compound requiring further study in the exhaled breath as it may improve diagnostic of Ps. aeruginosa infection when combined with other reported volatile markers.

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Section: Discussionmentioning

confidence: 99%

Volatile biomarkers of Pseudomonas aeruginosa in cystic fibrosis and noncystic fibrosis bronchiectasis

Savelev

Perry

Bourke

et al. 2011

Letters in Applied Microbiology

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“…Volatile organic compounds related to different pathogens have previously been linked with inflammatory effects (Schöller et al, 1997;Labows et al, 1980). Alkane, as well as methylated alkane VOCs, have been proposed as biomarkers of oxidative stress activity (Phillips et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Volatile organic compounds generated by cultures of bacteria and viruses associated with respiratory infections

Qader

Lieberman

Avni

et al. 2015

Biomedical Chromatography

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Respiratory infections (RI) can be viral or bacterial in origin. In either case, the invasion of the pathogen results in production and release of various volatile organic compounds (VOCs). The present study examines the VOCs released from cultures of five viruses (influenza A, influenza B, adenovirus, respiratory syncitial virus and parainfluenza 1 virus), three bacteria (Moraxella catarrhalis, Haemophilus influenzae and Legionella pneumophila) and Mycoplasma pneumoniae isolated colonies. Our results demonstrate the involvement of inflammation-induced VOCs. Two significant VOCs were identified as associated with infectious bacterial activity, heptane and methylcyclohexane. These two VOCs have been linked in previous studies to oxidative stress effects. In order to distinguish between bacterial and viral positive cultures, we performed principal component analysis including peak identity (retention time) and VOC concentration (i.e. area under the peak) revealing 1-hexanol and 1-heptadecene to be good predictors.

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“…fluorescens and Ps. putida (Labows et al 1980) and a cluster 3 pseudomonad (Dainty et al 1984). Such compounds play an important role in the development of the odour/flavour of blue cheeses (Dwivedi & Kinsella 1974) and it is therefore of some interest that 'cheesey' and 'dairy' were amongst the terms used to describe the earliest detectable non-fresh odours of naturally contaminated meat stored at chill temperatures (Dainty et al 1985).…”

Section: Discussionmentioning

confidence: 99%

Volatile compounds produced by meat pseudomonads and related reference strains during growth on beef stored in air at chill temperatures

Edwards

Dainty

Hibbard

1987

Journal of Applied Bacteriology

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Volatile compounds produced by 31 strains of pseudomonads and by reference strains of Pseudomonas fragi and Ps. fluorescens biotype 1 during growth on beef stored at 6 degrees C in air were analysed by gas chromatography-mass spectrometry of headspace gases. Compounds of major sensory significance were ethyl and methyl esters of C2-C8 fatty acids and sulphur-containing compounds which included methane- and isopropanethiols and their related sulphides and thioesters but not hydrogen sulphide. Ester production was mainly associated with growth of some, but not all, Ps. fragi and related meat strains but sulphur-containing compounds were produced by all but a single meat strain. A minority of other meat strains produced greater amounts of methyl ketones, secondary alcohols and unsaturated hydrocarbons believed to be of lipid origin.

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Headspace analysis of volatile metabolites of Pseudomonas aeruginosa and related species by gas chromatography-mass spectrometry

Cited by 153 publications

References 35 publications

Volatile biomarkers of Pseudomonas aeruginosa in cystic fibrosis and noncystic fibrosis bronchiectasis

Volatile biomarkers of Pseudomonas aeruginosa in cystic fibrosis and noncystic fibrosis bronchiectasis

Volatile organic compounds generated by cultures of bacteria and viruses associated with respiratory infections

Volatile compounds produced by meat pseudomonads and related reference strains during growth on beef stored in air at chill temperatures

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