Headspace Solid Phase Microextraction Coupled to GC/MS for the Analysis of Volatiles of Honeys from Arid and Mediterranean Areas of Algeria

Abstract

The volatile composition of seven honey samples collected from various regions of Algeria and feeding on different plants have been determined. The Headspace Solid‐Phase MicroExtraction (HS‐SPME) coupled with Gas Chromatography‐Mass Spectrometry (GC/MS) was used to achieve the purpose. In this work, different parameters of the HS‐SPME analytical method were investigated in order to reach maximal sensitivity, and thus to obtain maximum information about the volatile profile of Algerian honey. These parameters include saline medium, HS extraction temperature, and the nature of the fiber used. The results showed a great diversity in the chemical composition, in total 124 compounds from different chemical classes were identified, including compounds found for the first time in honey. The Ascending Hierarchical Classification (AHC) demonstrated the importance of choosing SPME extraction conditions to find volatile compounds, which could be as specific markers of the floral or geographical origin of honey, the latter was optimized in the parameter PDMS‐55 °C.

Botanical Origin, Pollen Profile, and Physicochemical Properties of Algerian Honey from Different Bioclimatic Areas

“…Some authors studied the characteristics of honey produced in Algeria and found differing results as to the pollen content [ 8 , 9 , 10 , 13 ], physicochemical attributes and pollen profile of samples from different areas [ 7 , 14 , 15 , 16 ], their botanical origin [ 9 , 11 , 17 , 18 , 19 ], antibacterial and antioxidant activity [ 20 ], or specific compounds such as sugars and phenolic or volatile compounds [ 21 , 22 ]. However, honey production in Algeria remains poorly studied.…”

Honey authenticity: analytical techniques, state of the art and challenges

“… 64 Other multi-coating fibers used in volatomic studies are PDMS/DVB and PDMS/CAR. Costa et al 65 distinguished honeys derived from different botanical and entomological origins, while Neggad et al 66 geographically differentiated Algerian honeys from Arid and Mediterranean Areas. Headspace analysis can be conducted in either a static or a dynamic way.…”

A new method of extracting polyphenols from honey using a biosorbent compared to the commercial resin amberlite XAD2

“…Article Related Abbreviation: BR, biodegradable resin monosaccharides (glucose and fructose) and disaccharides (composed of both fructose and glucose linked together). Honey also contains proteins, amino acids, vitamins, minerals, organic acids, alcohols, enzymes, lipids [2], volatiles [3,4], and other substances with a wide range of biological effects, such as antioxidants, and antibacterial and other therapeutic effects [5].…”