Healing of Full-Thickness Murine Skin Wounds Containing Nanofibers Using Splints for Efficient Reepithelialization and to Avoid Contracture

Bhattacharya, Nilika; Indra, Arup K.; Ganguli‐Indra, Gitali

doi:10.1007/978-1-0716-0655-1_10

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…It can plausibly be attributed to the adequate solubility of CUR–PYR that causes the produced ointment adheres easily to the wound bed and stays there for several hours. Reviewing previous literature shows that numerous advanced dressings have been suggested to help wound healing by providing balanced moisture to the wound site. ,,− Regarding the greater importance of re-epithelialization than contraction in human wound repair, on the other hand, several materials like foils, splints and human-mouse xenografts have been examined to avoid contraction during wound repair. − Surprisingly, our suggested drug without any supplementary dressing or tool is capable of keeping an open wound desirably hydrated coordinating wound contraction and re-epithelialization during wound healing. This prominent feature finally results in accelerated skin regeneration.…”

Section: Resultsmentioning

confidence: 99%

Accelerated Wound Healing with a Diminutive Scar through Cocrystal Engineered Curcumin

Samie,

Alavian,

Vafaei-Pour

et al. 2023

Mol. Pharmaceutics

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Pharmaceutical cocrystals (Regulatory Classification of Pharmaceutical Co-Crystals Guidance for Industry; Food and Drug Administration, 2018) are crystalline solids produced through supramolecular chemistry to modulate the physicochemical properties of active pharmaceutical ingredients (APIs). Despite their extensive development in interdisciplinary sciences, this is a pioneering study on the efficacy of pharmaceutical cocrystals in wound healing and scar reducing. Curcumin−pyrogallol cocrystal (CUR−PYR) was accordingly cherry-picked since its superior physicochemical properties adequately compensate for limitative drawbacks of curcumin (CUR). CUR− PYR has been synthesized by a liquid-assisted grinding (LAG) method and characterized via FT-IR, DSC, and PXRD analyses. In vitro antibacterial study indicated that CUR−PYR cocrystal, CUR+PYR physical mixture (PM), and PYR are more effective against both Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Staphylococcus aureus and Bacillus subtilis) bacteria in comparison with CUR. In vitro results also demonstrated that the viability of HDF and NIH-3T3 cells treated with CUR−PYR were improved more than those received CUR which is attributed to the effect of PYR in the form of cocrystal. The wound healing process has been monitored through a 15 day in vivo experiment on 75 male rats stratified into six groups: five groups treated by CUR−PYR+Vaseline (CUR−PYR.ung), CUR+PYR+Vaseline (CUR+PYR.ung), CUR+Vaseline (CUR.ung), PYR+Vaseline (PYR.ung), and Vaseline (VAS) ointments and a negative control group of 0.9% sodium chloride solution (NS). It was revealed that the wounds under CUR−PYR.ung treatment closed by day 12 postsurgery, while the wounds in other groups failed to reach the complete closure end point until the end of the experiment. Surprisingly, a diminutive scar (3.89 ± 0.97% of initial wound size) was observed in the CUR−PYR.ung treated wounds by day 15 after injury, followed by corresponding values for PYR.ung (12.08 ± 2.75%), CUR+PYR.ung (13.89 ± 5.02%), CUR.ung (16.24 ± 6.39%), VAS (18.97 ± 6.89%), and NS (20.33 ± 5.77%). Besides, investigating histopathological parameters including inflammation, granulation tissue, reepithelialization, and collagen deposition signified outstandingly higher ability of CUR−PYR cocrystal in wound healing than either of its two constituents separately or their simple PM. It was concluded that desired solubility of the prepared cocrystal was essentially responsible for accelerating wound closure and promoting tissue regeneration which yielded minimal scarring. This prototype research suggests a promising application of pharmaceutical cocrystals for the purpose of wound healing.

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Section: Resultsmentioning

confidence: 99%

Accelerated Wound Healing with a Diminutive Scar through Cocrystal Engineered Curcumin

Samie,

Alavian,

Vafaei-Pour

et al. 2023

Mol. Pharmaceutics

View full text Add to dashboard Cite

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“…The splint serves to mechanically hold the wound open, preventing the natural contraction of the wound edges and forcing healing by re-epithelialization. This model allows researchers to focus on specific aspects of healing without the confounding effects of wound closure by contraction [57,58]. Thus, one of the key advantages of this model is the limit on contraction, enabling the study of granulation tissue formation, re-epithelialization, and ECM deposition, thereby creating a more translational model.…”

Section: Splinted Full-thickness Wound Modelmentioning

confidence: 99%

Exploring Skin Wound Healing Models and the Impact of Natural Lipids on the Healing Process

Choudhary,

Bollag

2024

IJMS

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Cutaneous wound healing is a complex biological process involving a series of well-coordinated events aimed at restoring skin integrity and function. Various experimental models have been developed to study the mechanisms underlying skin wound repair and to evaluate potential therapeutic interventions. This review explores the diverse array of skin wound healing models utilized in research, ranging from rodent excisional wounds to advanced tissue engineering constructs and microfluidic platforms. More importantly, the influence of lipids on the wound healing process is examined, emphasizing their role in enhancing barrier function restoration, modulating inflammation, promoting cell proliferation, and promoting remodeling. Lipids, such as phospholipids, sphingolipids, and ceramides, play crucial roles in membrane structure, cell signaling, and tissue repair. Understanding the interplay between lipids and the wound microenvironment provides valuable insights into the development of novel therapeutic strategies for promoting efficient wound healing and tissue regeneration. This review highlights the significance of investigating skin wound healing models and elucidating the intricate involvement of lipids in the healing process, offering potential avenues for improving clinical outcomes in wound management.

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Healing of Full-Thickness Murine Skin Wounds Containing Nanofibers Using Splints for Efficient Reepithelialization and to Avoid Contracture

Cited by 2 publications

References 19 publications

Accelerated Wound Healing with a Diminutive Scar through Cocrystal Engineered Curcumin

Accelerated Wound Healing with a Diminutive Scar through Cocrystal Engineered Curcumin

Exploring Skin Wound Healing Models and the Impact of Natural Lipids on the Healing Process

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