Healing of osteochondral grafts in an ovine model under the influence of bFGF

Siebert, Christian H.; Miltner, Oliver; Weber, Michael H.; Sopka, Saša; Koch, S. W.; Niedhart, Christopher

doi:10.1053/jars.2003.50000

Cited by 45 publications

(35 citation statements)

References 22 publications

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“…Toolan et al (1998) and Gao et al (2002) reported integration of host and graft with hyaline-like tissue, in rabbit knees examined at 12 weeks. In sheep, Siebert et al (2003) observed radiographic solid integration at 3 months, but both macroscopic and microscopic examination failed to indicate cartilage integration. At 6 months, Siebert et al (2003) found that the bone was fully integrated, but not the cartilage.…”

Section: Discussionmentioning

confidence: 91%

“…In sheep, Siebert et al (2003) observed radiographic solid integration at 3 months, but both macroscopic and microscopic examination failed to indicate cartilage integration. At 6 months, Siebert et al (2003) found that the bone was fully integrated, but not the cartilage. Clinical experience with both autografts and allografts has shown a consistent repair and relief of pain in 75-85% of patients (Mahomed et al 1992); the positive outcome may result from bone integration, and fibrocartilaginous bridging of the gap between host and implant cartilage.…”

Section: Discussionmentioning

confidence: 91%

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The Repair Response to Osteochondral Implant Types in a Rabbit Model

Frenkel

Kubiak

Truncale

2006

Cell Tissue Banking

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Current treatments for damaged articular cartilage (i.e., shaving the articular surface, perforation or abrasion of the subchondral bone, and resurfacing with periosteal and perichondrial resurfacing) often produce fibrocartilage, or hyaline-appearing repair that is not sustained over time (Henche 1967, Ligament and Articular Cartilage Injuries. Springer-Verlag, New York, NY, pp. 157-164; Insall 1974, Clin. Orthop. 101: 61-67; Mitchell and Shepard 1976, J. Bone Joint Surg. [Am.] 58: 230-233; O'Driscoll et al. 1986, J. Bone Joint Surg. [Am.] 68: 1017-1035; 1989, Trans. Orthop. Res. Soc. 14: 145; Kim et al. 1991, J. Bone Joint Surg. [Am.] 73: 1301-1315). Autologous chondrocyte transplantation, although promising, requires two surgeries, has site-dependent and patient age limitations, and has unknown long-term donor site morbidity (Brittberg et al. 1994, N Engl. J. Med. 331: 889-895; Minas 2003, Orthopedics 26: 945-947; Peterson et al. 2003, J. Bone Joint Surg. Am. 85-A(Suppl. 2): S17-S24). Osteochondral allografts remain a widely used method of articular resurfacing to delay arthritic progression. The present study compared the histological response to four types of osteochondral implants in a rabbit model: autograft, frozen, freeze-dried, and fresh implants. Specimens implanted in the femoral groove were harvested at 6 and 12 weeks. Results showed similar restoration of the joint surface regardless of implant type, with a trend toward better repair at the later timepoint. As has been observed in other studies (Frenkel et al. 1997, J. Bone Joint Surg. 79B: 281-286; Toolan et al. 1998, J. Biomed. Mater. Res. 41: 244-250), each group in this study had at least one specimen in which a healthy-appearing surface on the implant was not well-integrated with host tissues. Although the differences were not statistically significant, freeze-dried implants at both timepoints had the best histological scores. The osteochondral grafts tested successfully restored the gross joint surface and congruity. At 12 weeks, no significant differences were observed between the various allografts and autologous osteochondral grafts.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 91%

The Repair Response to Osteochondral Implant Types in a Rabbit Model

Frenkel

Kubiak

Truncale

2006

Cell Tissue Banking

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“…It is reported that degeneration of well-formed articular cartilage repair tissue may occur within months or years after clinically successful improvement of joint function and because of this, for those methods that show promise at the earliest times another evaluation after 6 months is desirable, as performed in the present study. 13 From 6 to 12 months, a fibrocartilagineous tissue filled the defect of the spontaneously healing group.…”

Section: Histomorphometrical Analysismentioning

confidence: 98%

Transplantation of chondrocytes seeded on collagen‐based scaffold in cartilage defects in rabbits

Franceschi

Grigolo

Roseti

et al. 2005

J Biomedical Materials Res

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Recent success in tissue engineering by restoring cartilage defects by transplanting autologous chondrocyte cells on a three-dimensional scaffold has prompted the improvement of this therapeutic strategy. Here we describe a new approach investigating the healing of rabbit cartilage by means of autologous chondrocytes seeded on a biomaterial made of an equine collagen type I-based scaffold. Full-thickness defects were created bilaterally in the weight-bearing surface of the medial femoral condyle of both femora of New Zealand male rabbits. The wounds were then repaired by using both chondrocytes seeded on the biomaterial and biomaterial alone. Controls were similarly treated but received either no treatment or implants of the delivery substance. Histological examination of the reconstructed tissues at 1, 3, 6, and 12 months after transplantation showed that at 1 and 3 months there was no formation of reconstructed tissue in any of the groups evaluated; after 6 months there was evidence of a newly regenerated tissue with some fibrocartilaginous features only in the group treated with biomaterial-seeded cells, and at 12 months a more organized tissue was evident in the same group. With regards to the group transplanted with biomaterial alone and the untreated control group, there was no evidence of new tissue production. These results advocate the use of this collagen-based scaffold for further in vivo studies on large size animals and, finally, in human clinical trials for the treatment of knee cartilage defects.

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“…The slides were graded independently by three reviewers using the ICRS Visual Histological Scale, 34 the O'Driscoll Scale 35 and the semiquantitative Siebert score. 36 High-resolution micro-computed tomography ( CT) scans were made of all triphasic constructs (La Theta TM LCT-100, Aloka, Inc., Tokyo, Japan). The slice thickness was set to 0.1 mm.…”

Section: Surgical Techniquesmentioning

confidence: 99%

A novel MSC‐seeded triphasic construct for the repair of osteochondral defects

Marquaß

Somerson

Hepp

et al. 2010

Journal Orthopaedic Research

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Mesenchymal stem cells (MSC) are increasingly replacing chondrocytes in tissue engineering based research for treatment of osteochondral defects. The aim of this work was to determine whether repair of critical-size chronic osteochondral defects in an ovine model using MSC-seeded triphasic constructs would show results comparable to osteochondral autografting (OATS). Triphasic implants were engineered using a beta-tricalcium phosphate osseous phase, an intermediate activated plasma phase, and a collagen I hydrogel chondral phase. Autologous MSCs were used to seed the implants, with chondrogenic predifferentiation of the cells used in the cartilage phase. Osteochondral defects of 4.0 mm diameter were created bilaterally in ovine knees (n = 10). Six weeks later, half of the lesions were treated with OATS and half with triphasic constructs. The knees were dissected at 6 or 12 months. With the chosen study design we were not able to demonstrate significant differences between the histological scores of both groups. Subcategory analysis of O'Driscoll scores showed superior cartilage bonding in the 6-month triphasic group compared to the autograft group. The 12-month autograft group showed superior cartilage matrix morphology compared to the 12-month triphasic group. Macroscopic and biomechanical analysis showed no significant differences at 12 months. Autologous MSC-seeded triphasic implants showed comparable repair quality to osteochondral autografts in terms of histology and biomechanical testing.

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Healing of osteochondral grafts in an ovine model under the influence of bFGF

Cited by 45 publications

References 22 publications

The Repair Response to Osteochondral Implant Types in a Rabbit Model

The Repair Response to Osteochondral Implant Types in a Rabbit Model

Transplantation of chondrocytes seeded on collagen‐based scaffold in cartilage defects in rabbits

A novel MSC‐seeded triphasic construct for the repair of osteochondral defects

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