Healing potential of Calotropis procera on dermal wounds in Guinea pigs

Rasik, Anamika M.; Raghubir, Ram; Gupta, Asheesh; Shukla, A. N.; Dubey, Megha; Srivastava, Sushma; Jain, H.K; Kulshrestha, D. K.

doi:10.1016/s0378-8741(99)00118-x

Cited by 92 publications

(54 citation statements)

References 11 publications

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“…Breakdown of collagen liberates free hydroxyproline and its peptides. So the estimation of hydroxyproline is an accepted method of biochemically evaluating the total collagen content of a sample and also used as a marker of collagen synthesis (Lin et al, 2003;Rasik et al, 1992). A biochemical analysis of wound tissue in both the models demonstrated a significant increase in hydroxyproline content in successive ethanolic extract treated wounds compared to controlled wounds (Table 2).…”

Section: Resultsmentioning

confidence: 99%

Experimental wound healing aspects of <i>Jasminum grandiflorum</i>Linn: a preclinical study

Arun¹,

Satish²,

Anima³

2015

Afr. J. Trad. Compl. Alt. Med.

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Background: Jasminum grandiflorum is an important shrub of family Oleaceae and ancient literature mentions its various medicinal uses especially its efficacy in wound healing. To substantiate its traditional claim the present study was conceived. Materials and Methods: Preliminary wound healing activity of successive extracts of leaves was assessed by measuring wound contraction using excision wound model and the detailed study of most potent extract was evaluated by measuring wound contraction, epithelialization period, hydroxyproline content, tissue breaking strength and histopathology parameters by excision and incision wound models. The antioxidant activity was determined by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method. The agar well-diffusion method and minimum inhibitory concentration (MIC) was used to established antimicrobial efficacy. Results:The results show that successive ethanolic extract of the leaves exhibited promising wound healing, antioxidant and antimicrobial properties. Conclusion: From results; it is concluded that its traditional use for the treatment of wound and in microbial infection is justified.

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Section: Resultsmentioning

confidence: 99%

Experimental wound healing aspects of <i>Jasminum grandiflorum</i>Linn: a preclinical study

Arun¹,

Satish²,

Anima³

2015

Afr. J. Trad. Compl. Alt. Med.

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“…The estimation of hydroxyproline is an accepted method of biochemical evaluation of total collagen content of a sample [22] and is also used as a marker of collagen synthesis [23]. An increase in the collagen content of the extracellular matrix is a characteristic change observed in the proliferative phase of wound healing [22].…”

Section: Discussionmentioning

confidence: 99%

Wound healing potential of Capparis spinosa against cutaneous wounds infected by Escherichia coli in a rat model

et al. 2015

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S u m m a r yWound infection has become a major medical problem in recent years. This study was conducted to evaluate the healing activity of Capparis spinosa against surgical wounds infected by Escherichia coli. Twenty male rats were divided into two groups. Excisions were created surgically on the animals' skin and then infected with E . coli. Group 1 was treated with C . spinosa while Group 2 was untreated. Wound biopsy specimens were collected on days 5, 10 and 16 and analyzed. Results showed that the hydroxyproline content in treatment group was significantly higher in various post wounding days. Protein content increased gradually in ten days. Results of histopathological studies showed moderate to intense granulation tissue formation in treated group on day 10. The histopathological studies showed, that the new epidermis in treated group was thicker than in control group on day 16 post wounding. The present study has demonstrated that ethanol extract of C . spinosa includes properties that accelerate wound healing activities. INTRODUCTIONWound is defined simply as the disruption of the cellular and anatomic continuity of a tissue. Wound may be produced by physical, chemical, thermal, microbial or immunological insult to the tissue. Wound healing is a programmed biological process that restores tissue continuity after injury and is a combination of physical, chemical and cellular events that restore the wounded tissue or replace it with collagen. Wound healing can be divided into three stages, including inflammation, proliferation and remodeling and maturation phases which involve the interaction of various cells, cytokines and growth factors [1]. The normal healing response begins immediately after the injury. When blood spills into the site of injury, the blood platelets contact with collagen and other elements of the extracellular matrix. This triggers the release of clotting factors as well as essential growth factors and cytokines such as platelet-derived growth factor (PDGF) and transforming growth factor beta (TGF-β). The inflammatory phase begins after the migration of neutrophils to the wound site to clean the tissue. Then, fibroblasts migrate into the tissue to begin the proliferative phase and deposit new extracellular matrix. This new collagen matrix becomes cross linked and organized [2].Due to poor hygienic conditions in undeveloped and developing countries, wound infection has become a common disease in recent years [3]. Several reports associating the enterobacterium Escherichia coli with skin and soft tissue infections have been published: E . coli was found to be the causative agent of neonatal omphalitis [4], cellulitis localized to lower or upper limbs [5], necrotizing fasciitis [6,7], surgical site infections [8], infections after burn injuries [9]. A public tendency towards the use of herbal wound healers is increasing, possibly due to lower side effects and prices of herbals, compared to chemical drugs.Capparis spinosa (caper) (family Capparidaceae) is one of the most common aromati...

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“…Guinea pigs were used, as these are a common animal model in research on cutaneous wound healing [18][19][20][21][22][23][24][25]. Although their skin structure is reported to be similar to human skin [18], some apparent differences still exist.…”

Section: Discussionmentioning

confidence: 99%

The influence of a PHI-5-loaded silicone membrane, on cutaneous wound healing in vivo

Rossum

Vooijs

Walboomers

et al. 2007

J Mater Sci: Mater Med

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This study investigated whether a novel ionogenic substance, containing amongst others zinc and rubidium (PHI-5; Dermagenics Inc, Memphis, TN, USA), could improve the healing of full-thickness skin wounds. Uniform wounds were created on the right flank of guinea pigs. Micro-grooved silicone rubber membranes, containing 0 (controls), 1.25, 5.00, or 10.00 lg PHI-5, were sutured onto this wound. Standardized digital wound photographs were made after 1, 3, and 6 weeks. Also, wound biopsies were taken after 3 and 6 weeks for histological and histomorphometrical evaluation. For all study groups, 6 animals were used. Analysis of the 1-week digital photographs showed that the surface area of the wounds decreased significantly, with an increasing PHI-5 concentration. No other differences were found in the wound photographs. Also, no differences were measured in histomorphometry at 3 and 6 weeks. Concluding, in our study model a single application of PHI-5 did have a significant positive influence on initial wound healing.

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Healing potential of Calotropis procera on dermal wounds in Guinea pigs

Cited by 92 publications

References 11 publications

Experimental wound healing aspects of <i>Jasminum grandiflorum</i>Linn: a preclinical study

Experimental wound healing aspects of <i>Jasminum grandiflorum</i>Linn: a preclinical study

Wound healing potential of Capparis spinosa against cutaneous wounds infected by Escherichia coli in a rat model

The influence of a PHI-5-loaded silicone membrane, on cutaneous wound healing in vivo

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