Healing Process in Mice Model of Surgical Wounds Enhanced by Phyllocaulis boraceiensis Mucus

Toledo-Piza, Ana Rita de; Maria, Durvanei Augusto

doi:10.1097/01.asw.0000456683.55591.af

Cited by 9 publications

(11 citation statements)

References 26 publications

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“…One of the aspects evaluated in the present article is that endothelial cells treated with P. boraceiensis mucus undergo significant changes in the expression of these receptors for vascular growth, which is possibly one of the reasons for the efficacy of treatment in surgical wounds. P. boraceiensis mucus was shown to be an efficient compound to accelerate the wound healing process in mice treated with 0.012 μg·μL −1 P. boraceiensis mucus, which is the same concentration as used in our experiments .…”

Section: Discussionmentioning

confidence: 96%

Angiogenesis enhanced by Phyllocaulis boraceiensis mucus in human cells

Toledo-Piza

Maria

2013

The FEBS Journal

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Phyllocaulis boraceiensis mucus is known to be a compound capable of inducing cell proliferation and enhancing the wound healing process. The process of angiogenesis is a chain of mechanisms responsible for the formation of new vessels, which are are involved in cell proliferation, and factors that will act in the healing process. Our aim was to demonstrate that the angiogenesis process is enhanced in cultures of endothelial cells and fibroblasts treated with P. boraceiensis mucus. Experiments were carried out with 10 5 cellsÁmL À1 of endothelial cells and fibroblasts treated with P. boraceiensis mucus in concentrations that have significant effects in proliferation assays, i.e. 0.012 lgÁlL À1 and 0.18 lgÁlL À1 , both of which cause extreme responses. Aliquots of 100 lL of cell suspensions were incubated for 1 h at 4°C with 1 lL of antibodies specific for the cell markers vascular endothelial growth factor receptor 1 and cluster of differentiation 34, and negative isotype controls. Reading and expression analysis of cell markers was performed on a FACSCalibur flow cytometer. Expression levels of vascular endothelial growth factor receptor 1 and cluster of differentiation 34 expression were significantly increased in endothelial cells cultivated with 0.012 lgÁlL À1 P. boraceiensis mucus, suggesting that this compound is capable of enhancing angiogenesis.

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Section: Discussionmentioning

confidence: 96%

Angiogenesis enhanced by Phyllocaulis boraceiensis mucus in human cells

Toledo-Piza

Maria

2013

The FEBS Journal

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“…In our study, we investigated the topical effects of centaury oil on wounded area and also we studied the molecular effects of this extract on the genes VEGFA, VEGFB, VEBGFC, PDGFB and FGF2. 12 . Süntar et all applied centaury oil only in the first seven days after the surgery (proliferation phase) 2 .…”

Section: Discussionmentioning

confidence: 99%

“…In this clinically evaluation, edema, hyperemia, bleeding, granulation tissue, scar uncertainty and refeathering of the wound areas were observed 12 .…”

Section: Investment Of Wound Healingmentioning

confidence: 99%

KANTARON YAĞI VEGFA, VEGFB, VEGFC ve FGF2 GENLERİ ÜZERİNDEN ERKEN DÖNEM KULLANILDIĞINDA YARA İYİLEŞMESİNDE OLUMLU ETKİ GÖSTERMEKTEDİR

Çoban¹,

Yener²,

Benli³

et al. 2016

Cumhuriyet Medical Journal

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SUMMARYCentaury oil (the extract of Hypericum perforatum obtained with olive oil) is traditionally used for the treatment of wounds in Turkey. Centaury oil has analgesic and anti-infectious properties beside wound healing effect. There are many studies about the positive effect of this extract on wound healing in literature. But there is no mention about the animal studies that show which pathways are involved in. In our study the effect of centaury oil in early stages (first 7 days) and late stages (second 7 days) of wound healing was showed on the mice (mus. musculus) by creating sterile sutures on their back. Due to our results, centaury oil has positive effects on the surface of the wounds in early stages but has no effect on late stages. This information is explained as centaury oil can be useful if it is used in the first week of the wound. In addition, the gene expression profiles of VEGFA, VEGFB, VEGFC, PDGFB and FGF2 genes which have roles in wound healing via angiogenesis, were studied by RT-PCR method. As a result, the gene expression levels of VEGFA, VEGFB, VEGFC and FGF2 were increased in early stages of wound healing but only VEGFA gene expression level was increased in late stages. The gene expression findings were correlated with morphological findings in both early and late stages of wound healing. Our findings are important for exploring the effect of centaury oil on angiogenesis via VEGFA, VEGFB, VEGFC and FGF2 genes in early stages of wound healing. Keywords : Centaury oil, Hypericum perforatum L., wound healing, angiogenesis, gene ÖZET Kantaron yağı (Hypericum perforatum' un zeytinyağı ile elde edilen ekstresi) Türkiye' de yıllardır yara tedavisinde geleneksel olarak kullanılmaktadır. Kantaron yağının yara iyileştirici özellikleri yanında ağrı kesici, enfeksiyon engelleyici özellikleri bulunmaktadır. Literatürde bu yağın yara iyileşmesine olan pozitif etkisi ile ilgili birçok çalışma vardır. Ancak literatürde şimdiye dek bu özelliklerin hangi yolaklar üzerinden olduğunu gösterir hayvan deneylerini içeren detaylı bir çalışma bulunmamaktadır. Çalışmamızda kantaron yağının yara iyileşmesi üzerine erken dönem (ilk 7 gün) ve geç dönem (ikinci 7 gün) etkisi farelerde (mus. musculus) sırt bölgesinde steril sütürler oluşturularak gösterilmiştir. Sonuçlarımıza göre, farelerde oluşturulan yara yüzeyinde morfolojik olarak kantaron yağının erken dönem kullanımda pozitif etkisi olmakta, geç dönem uygulamada bu etki görülmemektedir. Bu bulgu kantaron yağının yara iyileşmesine yara oluştuktan sonraki ilk haftada pozitif etkisi olduğu şeklinde yorumlanmıştır. Bu dönemde alınan dokulardan RT-PCR yöntemi ile anjiyogenez üzerinden yara iyileşmesinde rolü olan VEGFA, VEGFB, VEGFC, PDGFB ve FGF2 genlerinin gen ekspresyon profilleri incelenmiştir. Sonuç olarak erken dönemde VEGFA, 232 CMJCumhuriyet Medical Journal VEGFB, VEGFC ve FGF2 genlerinin gen ekspresyonları artmakta, geç dönemde bu etki yalnızca VEGFA geninde saptanmaktadır. Erken ve geç dönemde bulunan gen ekspresyonu bulguları saptanan morfolojik özellik...

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“…In this clinically evaluation, edema, hyperemia, bleeding, granulation tissue, scar uncertainty and refeathering of the wound areas were observed (12). Tissue sample preperation: In the 7 th day of study, the wound areas of three mice from control group and five mice from group A were completely taken for RNA isolation.…”

Section: Investment Of Wound Healingmentioning

confidence: 99%

“…Remodeling begins in the 6 th day. Granulation and scar tissues occurs in 13 th day (12). Süntar et al applied centaury oil only in the first seven days after the surgery (proliferation phase) (2).…”

Section: Cmjmentioning

confidence: 99%

Molecular view to the effect of centaury oil on wound healing

Çoban¹,

Yener²,

Benli³

et al. 2016

Cumhuriyet Med J

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SUMMARYObjective: Centaury oil (the extract of Hypericum perforatum obtained with olive oil) is traditionally used for the treatment of wounds in Turkey. Centaury oil has analgesic and antiinfectious properties beside wound healing effect. There are many studies about the positive effect of this extract on wound healing in literature. But there is no mention about the animal studies that show which pathways are involved in. Method: In our study the effect of centaury oil in early stages (first 7 days) and late stages (second 7 days) of wound healing was showed on the mice (mus. musculus) by creating sterile sutures on their back. In addition, the gene expression profiles of VEGFA, VEGFB, VEGFC, PDGFB and FGF2 genes which have roles in wound healing via angiogenesis were studied by RT-PCR method. Results: Due to our results, centaury oil has positive effects on the surface of the wounds in early stages but has no effect on late stages. The gene expression levels of VEGFA, VEGFB, VEGFC and FGF2 were increased in early stages of wound healing but only VEGFA gene expression level was increased in late stages. The gene expression findings were correlated with morphological findings in both early and late stages of wound healing. Conclusions: According to our morphological results, centaury oil can be useful if it is used in the first week of the wound. At the end of our study, our findings are important for exploring the effect of centaury oil on angiogenesis via VEGFA, VEGFB, VEGFC and FGF2 genes in early stages of wound healing. Keywords: Centaury oil, Hypericum perforatum L., wound healing, angiogenesis, gene. ÖZET Amaç: Kantaron yağı (Hypericum perforatum' un zeytinyağı ile elde edilen ekstresi) Türkiye' de yıllardır çeşitli yaraların tedavisinde geleneksel olarak kullanılmaktadır. Kantaron yağının yara iyileştirici özellikleri yanında anti-enflamatuar ve anti-inflamatuar özellikleri bulunmaktadır. Literatürde bu yağın yara iyileşmesine olan pozitif etkisi ile ilgili birçok çalışma vardır. Ancak literatürde şimdiye dek bu özelliklerin hangi yolaklar üzerinden olduğunu gösterir hayvan deneylerini içeren detaylı bir çalışma bulunmamaktadır. Yöntem: Çalışmamızda kantaron yağının yara iyileşmesi üzerine erken dönem (ilk 7 gün) ve geç dönem (ikinci 7 gün) etkisi; farelerde (mus. musculus) sırt bölgesinde steril sütürler oluşturulup farklı zaman aralıklarında kantaron yağı uygulanarak gösterilmeye çalışılmıştır. Çalışmamızda ayrıca bu dönemde tüm gruplardan alınan dokulardan Real Time PCR yöntemi ile anjiyogenez üzerinden yara iyileşmesinde aktif rol oynayan VEGFA, VEGFB, VEGFC, PDGFB ve FGF2 132 CMJCumhuriyet Medical Journal genlerinin gen ekspresyon profilleri incelenmiştir. Bulgular: Elde ettiğimiz sonuçlara göre, farelerde oluşturulan yara yüzeylerinde morfolojik olarak kantaron yağının erken dönem kullanımında yara iyileşmesine pozitif etkisi olmakta, geç dönem uygulanmasında ise yara iyileştirici etkisi görülmemektedir. Yara iyileşmesinin erken dönemlerinde (akut dönem) VEGFA, VEGFB, VEGFC ve FGF2 genlerinin gen ...

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Healing Process in Mice Model of Surgical Wounds Enhanced by Phyllocaulis boraceiensis Mucus

Abstract: The healing process and wound closure were efficient after the daily application of 0.012 μg/μL P boraceiensis mucus.

Cited by 9 publications

References 26 publications

Angiogenesis enhanced by Phyllocaulis boraceiensis mucus in human cells

Angiogenesis enhanced by Phyllocaulis boraceiensis mucus in human cells

KANTARON YAĞI VEGFA, VEGFB, VEGFC ve FGF2 GENLERİ ÜZERİNDEN ERKEN DÖNEM KULLANILDIĞINDA YARA İYİLEŞMESİNDE OLUMLU ETKİ GÖSTERMEKTEDİR

Molecular view to the effect of centaury oil on wound healing

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