Healing wounds, defeating biofilms: Lactiplantibacillus plantarum in tackling MRSA infections

Abstract

IntroductionMethicillin-resistant Staphylococcus aureus (MRSA) infections are well-known hospital-borne infections and are a major contributing factor to global health concerns of antimicrobial resistance due to the formation of biofilms. Probiotics are known to assist in the healing of wounds through immunomodulation and also possess anti-pathogen properties via competitive inhibition. The probiotic bacterium, Lactiplantibacillus plantarum MTCC 2621 and its cell-free supernatant (Lp2621) have previously been reported to have antibacterial, excellent antioxidant, and wound healing activity in in vitro conditions and wounds contaminated with S. aureus in mice.MethodsIn the current study, we evaluated its anti-MRSA, biofilm inhibition and eradication efficacy, immunomodulatory activity in THP-1 cells, and wound healing potential in wounds contaminated with MRSA infection in mice.ResultsIn agar well diffusion assay, Lp2621 showed anti-MRSA activity and revealed dose-dependent inhibition and eradication of biofilm by crystal violet assay as well as by Confocal Scanning Laser Microscopy (CLSM) analysis. Further, Lp2621 showed immunomodulatory activity at varied concentrations as measured by IL-6 and IL-10 gene expression in THP-1 cells. Similar findings were observed in serum samples of mice after treatment of excision wound contaminated with MRSA infection by Lp2621 gel, as evident by expression of IL-6 (pro-inflammatory) and IL-10 (anti-inflammatory) cytokines.ConclusionsOverall, our results show that Lp2621 has potent anti-MRSA and antioxidant properties and can prevent and eliminate biofilm formation. It also showed promise when applied to mice with MRSA-infected wounds.

Daptomycin Liposomes Exhibit Enhanced Activity against Staphylococci Biofilms Compared to Free Drug

“…Between the major unmet medical needs that currently represent serious public health threats, methicillin-resistant Staphylococcus aureus (MRSA) infections (which are associated with high mortality rates) and, particularly, medical device-associated infections (MDIs), are of major concern [ 1 , 2 , 3 , 4 ]. MDIs are caused by a patient’s microbiota flora having a biofilm-forming ability.…”

Daptomycin Liposomes Exhibit Enhanced Activity against Staphylococci Biofilms Compared to Free Drug

“…Between the major unmet medical needs that currently represent serious public health threats, methicillin-resistant Staphylococcus aureus (MRSA) infections (which are associated with high mortality rates) and, particularly, medical device-associated infections (MDIs), are of major concern [1][2][3][4]. MDIs are caused by a patient's microbiota flora having a biofilmforming ability.…”