Background: Cultural Competence (CC) is an effective approach to reducing racial and ethnic inequalities in the provision of healthcare by relevant staff. A valid and reliable scale is one of the main tools for situation analysis and planning CC promotion programs; this study investigated the psychometric properties of the Cultural Competence Scale (CCS) among Iranian healthcare providers. Objectives: This study was done with the aim of the psychometric analysis of the Cultural Competence Scale (CCS) among Iranian health care providers. Materials and Methods: This cross-sectional study was performed among 192 healthcare providers who worked at healthcare centers of Kermanshah City, Iran, in 2018. The CCS was prepared by the assessment of the international CC questionnaires. Cronbach's α coefficient and exploratory factor analysis were used to measure the reliability and construct validity of it, respectively. Results: Kaiser-Meyer-Olkin (KMO) was calculated as 0.817. Bartlett's sphericity test was statistically significant (P<0.001). Five factors were extracted based on the eigenvalues of ≥1 and factor loading of ≥0.4. Based on the conceptual basis of CCS, 5 factors were included; cultural skill, cultural awareness, cultural desire, cultural encounters, and cultural behavior, which accounted for 62.44% of the assumed model. The Cronbach's α coefficient was calculated equal to 0.85, 0.80, 0.80, 0.71, and 0.70, respectively. Conclusion: CCS was reliable and valid and can be useful in planning CC promotion programs among Iranian healthcare providers.