Bridges play a crucial role in the development of the national economy and transportation industry, and their deformation monitoring is vital for ensuring their health. Therefore, it is necessary to conduct long-term monitoring of bridges’ deformation. This study monitored the deformation of the Wuhan Yangtze River Bridge using the SBAS-InSAR technology and Sentinel-1A data. The deformation results were analyzed in combination with bridge structure, human activity, temperature and stratigraphy. The results were as follows: (1) The vertical deformation rate of the bridge was between −15.6 and 10.7 mm/year, and part of the deformation belonged to rebound deformation; (2) The middle span deformation is the largest and the uplift and lowering alternate; (3) The reduction in human activity is the reason for the lower deformation amplitude from January to October 2020 compared to after October 2020; (4) A positive correlation between deformation and temperature was observed only along a portion of the bridge; (5) There is no direct correlation between observed lowering and stratigraphy under the bridge piers, as the sinking is presumably absorbed by the bridge structure.