This study aims to examine the educational needs and knowledge gaps of healthcare professionals in treating Acute Myocardial Infarction (AMI), a leading cause of death worldwide due to its impact on the heart muscles and blood vessels. Although healthcare workers generally understand how to manage AMI, there are crucial areas where their knowledge could be improved. The research involved 65 healthcare professionals from Jordan University Hospital, collecting data through surveys with structured questions and analyzing it with descriptive statistics. The findings indicate that while nurses are proficient in recognizing symptoms and managing care for AMI patients, including medication administration and patient education, they require more resources and training. Specifically, they need better training in oxygen therapy, emergency procedures, and advising patients on lifestyle changes. The study highlights the need for targeted, patient-focused education to address these gaps and improve AMI management in hospitals.