Introduction: HIV causes morbidity and mortality in infants and children, pertaining to its motherto-child transmission (MTCT) risk. MTCT which occur during pregnancy, labour and breastfeeding, is responsible for 90% childhood HIV infection. The main objective of the prevention of mother-to-child transmission (PMTCT) is to reduce the transmission of HIV infection from HIV infected mothers to their off springs. AIM:Our objective was to determine the level of HIV/AIDS knowledge of pregnant women. Methods: This study was done on pregnant mother at the antenatal clinic at Shri Vasantrao Naik Government Medical College & hospital (SVNGMC). One hundred and fifty mothers were interviewed at the antenatal clinics. It is non-experimental, cross-sectional type of study. Statistical Analysis:Comparison of categorical data with education and occupation was done using the Chi-Square test. Results: Out of 150, most of the women belongs to 21-25yrs age group (54.00 %),55.34 %were housewife. 95.34 %were literate. Television & radio were more strongly related to knowledge of HIV/AIDS. 77.34 % mother knew that it is transmitted by sexual route and 22.66 % mother did not know the transmission of HIV/AIDS. CONCLUSION: Education and correct scientific information are urgently needed to pregnant women to avoid myths and misconception on HIV/AIDS.