Health status and bioremediation capacity of wild freshwater mussels (Diplodon chilensis) exposed to sewage water pollution in a glacial Patagonian lake

Bianchi, Virginia Angélica; Castro, Juan Manuel; Rocchetta, Iara; Bieczynski, Flavia; Luquet, Carlos Marcelo

doi:10.1016/j.fsi.2014.02.013

Cited by 28 publications

(29 citation statements)

References 85 publications

(105 reference statements)

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“…Total and viable hemocytes were counted in Neubauer's chamber using Trypan Blue (0.2%) dissolved in sterile anticoagulant solution (3 g glucose and 0.36 g trisodium citrate per L, pH 7), according to Bianchi et al [48]. Total hemocyte were expressed as cells/mL of hemolymph and viability was expressed as the proportion of viable cells (not stained with Trypan Blue)/total cells [24].…”

Section: Experimental Designmentioning

confidence: 99%

“…Total hemocyte were expressed as cells/mL of hemolymph and viability was expressed as the proportion of viable cells (not stained with Trypan Blue)/total cells [24]. Hemocyte phagocytic activity was evaluated against Congo red stained yeast cells and expressed as the phagocytized yeast cells/hemocyte ratio [48].…”

Section: Experimental Designmentioning

confidence: 99%

“…Hemolymph was centrifuged at 500Â g for 20 min (4 C) and bacteriolytic activity (BA) of hemolymph supernatant was measured against E. coli JM109 strain at 1.5 Â 10 8 cell/mL [48] and expressed as BA/mL. This assay allows measuring the total lytic activity in the sample, since the decrease in absorbance values indicates the breakdown of bacteria cells.…”

Section: Humoral Responsementioning

confidence: 99%

“…Measurements of BA/mg of protein were made following the same procedure as for plasma samples. The morphometric relationships (n ¼ 6 per treatment), gill factor (GF) and digestive gland factor (DGF) were calculated for each mussel according to Bianchi et al [48]. The organ wet mass (g) was divided by the shell length 3 (cm) and multiplied by 100.…”

Section: Tissue Measurementsmentioning

confidence: 99%

See 3 more Smart Citations

Long-term feeding with Euglena gracilis cells modulates immune responses, oxidative balance and metabolic condition in Diplodon chilensis (Mollusca, Bivalvia, Hyriidae) exposed to living Escherichia coli

Bianchi

Castro

Rocchetta

et al. 2015

Fish & Shellfish Immunology

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Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Humoral Responsementioning

confidence: 99%

Section: Tissue Measurementsmentioning

confidence: 99%

See 2 more Smart Citations

Long-term feeding with Euglena gracilis cells modulates immune responses, oxidative balance and metabolic condition in Diplodon chilensis (Mollusca, Bivalvia, Hyriidae) exposed to living Escherichia coli

Bianchi

Castro

Rocchetta

et al. 2015

Fish & Shellfish Immunology

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“…İnsan ve hayvan atıklarını içeren lağım suları, deniz kıyı sularına veya yeraltı suları içine çeşitli insan patojenlerini taşır. Lağım suyu, çok fazla sayıda bakteri, enterik virüsleri ve diğer mikroorganizmaları içerir [1,2,3]. Avrupa Birliği Komisyonu 2006 yılında aldığı bir kararda rekreasyonel kullanıma açık alanlarda Salmonella spp., tespitinin yapılması ve bir litre deniz suyundaki Salmonella spp., sayısının litrede sıfır olması gerektiğine karar vermiştir.…”

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Kara Midye’lerin (Mytilus galloprovincialis) Gram-Negatif Bakteri Florasının Tespiti

Kayhan¹,

Sesal²,

Güldür³

2016

Marmara Fen Bil Dergisi

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ÖZBu çalışmanın amacı, İstanbul Boğazı'ndan toplanan ve besin olarak sıklıkla tüketilen Kara midye'lerin (Mytilus galloprovincialis) solungaç ve bağırsaklarındaki gram-negatif bakteri florasının tayin edilmesidir. Midye örnekleri İstanbul Boğazı'nda belirlenen 4 istasyondan (Haliç, Karaköy, Kadıköy, Üsküdar) elle toplanmıştır. Kara midye örneklerinin bağırsak ve solungacından 17 adet gram-negatif bakteri izole edilmiş ve türlerin identifikasyonu yapılmıştır. Daha sonra tüm koloniler morfolojilerine göre değerlendirildikten sonra tiplendirme işlemi API (Biomerieux) bakteri tanımlama sistemi kullanılarak yapılmıştır. Besin olarak sıklıkla tüketilen midyelerin organ ve dokularında patojen bakteri türlerinin bulunması ve bu bakterilerin ağır metale karşı dirençli olmaları, İstanbul Boğazı'nın ekolojik kirliliği hakkında önemli bulgular sağlamıştır. Anahtar kelimeler: Gram-negatif bakteri florası, Mytilus galloprovincialis, Mikrobiyal deniz kirliliği I. GİRİŞSon yıllardaki kontrolsüz kentleşme ve endüstrileşme ülkemiz denizlerinin kirlenmesine sebep olmuştur. Arıtılmamış veya yetersiz arıtılarak deşarjı yapılan sular, doğrudan doğruya kıyı çevrelerini kirleten kaynaklar arasındadır. İnsan ve hayvan atıklarını içeren lağım suları, deniz kıyı sularına veya yeraltı suları içine çeşitli insan patojenlerini taşır. Lağım suyu, çok fazla sayıda bakteri, enterik virüsleri ve diğer mikroorganizmaları içerir [1,2,3]. Avrupa Birliği Komisyonu 2006 yılında aldığı bir kararda rekreasyonel kullanıma açık alanlarda Salmonella spp., tespitinin yapılması ve bir litre deniz suyundaki Salmonella spp., sayısının litrede sıfır olması gerektiğine karar vermiştir. Deniz sularının litresinde 7.000 koliform bakteri kritik sınır olarak kabul edilir ve eğer bu sayı 10.000 olursa alanın kullanımının yasaklanması gerekmektedir [4]. Örneğin, tarım alanlarının kanalizasyon suyu ile sulanması veya kanalizasyon sularının akarsu, göl ve denizlere boşaltılması sonucu kanalizasyon sularında bulunan hastalık yapıcı mikroorganizmalar toprağa, denizlere ve atmosfere geçerek çevre kirlenmesine yol açar. Denizlerdeki patojen mikroorganizmaların her birinin varlığını tespit etmek için The Identification of Gram-Negative Bacterial Flora of Black Mussel (Mytilus galloprovincialis) ABSTRACTThe aim of this study is the identification of gram negative bacterial species in the intestine and gill tissues of the mussels (Mytilus galloprovincialis Lamarck, 1819). Mussel samples were collected from four stations (Halic, Karakoy, Kadıkoy, Uskudar) of Bosphorus.Following the evaluation of all colonies according to their morphologies, API (Biomerieux) bacterial identification system was used for typology. Seventeen gram-negative bacterial species have been isolated and identified from the intestine and gills of the mussel samples. Presence of heavy metal resistant pathogenic bacteria species into organ and tissue of the mussels which consume by people as a supplemental food, have been provided significant findings on ecological pollution of Bosphorus (İstanbul).

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Long‐ term effects of water quality on the freshwater bivalve Diplodon chilensis (Unionida: Hyriidae) caged at different sites in a North Patagonian river (Argentina)

et al. 2020

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Water quality was monitored along an Andean river of global importance using the freshwater bivalve Diplodon chilensis as sentinel species. Bivalves were placed in cages at three sites (S1–3) in the Chimehuin River in order to evaluate the long‐term effects of a trout hatchery (S2) and the open dump and sewage treatment plant of a nearby city (S3). Water samples and bivalves were collected at 0, 3, 6, 9 and 12 months, from S3, S2 and from a reference site upstream (S1), and physicochemical parameters and the biological response of the caged bivalves were studied. ROS production, antioxidant response, oxidative damage, energy status and morphometric ratios were included as response biomarkers. Most of biomarkers showed site and time dependence including bivalves transplanted at S1, revealing natural variability. Both anthropogenic perturbed sites (S2 and S3) showed differences in the exposed bivalves with respect to the reference site (S1) in their biomarker responses after 9 and 12 months of exposure (July and September, respectively). Multivariate analysis showed alterations in hemocytes (ROS, TOSC and NRRT50), in energy balance in digestive gland (energy and DGF) and in the detoxification response (GST) and GSH values in gill when bivalves were exposed to hatchery waste; whereas metal and bacterial pollution (S3 in July) caused GSH increase and a reduction of lysosomal damage in hemocytes. Results show that changes in the water quality of Chimehuin River due to the anthropogenic impact can be detected using the biomarkers analysed on D. chilensis, being a useful tool for studies of long‐term monitories.

show abstract

Health status and bioremediation capacity of wild freshwater mussels (Diplodon chilensis) exposed to sewage water pollution in a glacial Patagonian lake

Cited by 28 publications

References 85 publications

Long-term feeding with Euglena gracilis cells modulates immune responses, oxidative balance and metabolic condition in Diplodon chilensis (Mollusca, Bivalvia, Hyriidae) exposed to living Escherichia coli

Long-term feeding with Euglena gracilis cells modulates immune responses, oxidative balance and metabolic condition in Diplodon chilensis (Mollusca, Bivalvia, Hyriidae) exposed to living Escherichia coli

Kara Midye’lerin (Mytilus galloprovincialis) Gram-Negatif Bakteri Florasının Tespiti

Long‐ term effects of water quality on the freshwater bivalve Diplodon chilensis (Unionida: Hyriidae) caged at different sites in a North Patagonian river (Argentina)

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