A b s t r a c t Nosocomial infections are a serious problem not only in Slovakia but in all countries. The European Commission decided on their standardized surveillance in the whole European Union. According methodology elaborated by experts from the European Centre for Disease Control and Prevention in Stockholm we performed a point prevalence survey in the University Hospital Martin.Our observed prevalence of nosocomial infections in University Hospital Martin within the point prevalence study was 5.2%.The highest point prevalence of nosocomial infections was found at the Surgical Department (9.3%). The most common type of nosocomial infections was urological infections (27.3%), sepsis (22.7%) and surgical site infection (22.7%).The most common microorganisms isolated from the biological material were Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus mirabilis. Appropriate method of nosocomial infections surveillance is monitoring their prevalence in the point prevalence studies. International projects of nosocomial infections in the EU allow to compare the obtained results with other hospitals in the Member States.