Background: Burnout syndrome occurs in people who work in jobs which involve frequent and intense contact with people, especially healthcare workers. High dependency departments such as critical care units are very stressful environments, and this can lead to a greater incidence of burnout, especially of emotional exhaustion and poor personal accomplishment. Nurses are the largest group of healthcare workers, and so it is reasonable to expect they would have a high prevalence of burnout.Subjects and methods: The aim of this study was to assess the prevalence of burnout in critical care nurses in Croatia and explore its association with demographic features. A cross-sectional study of 620 nurses was conducted in several university hospitals, using convenience sampling. The Maslach Burnout Inventory was administered, together with questions about the sociodemographic and work characteristics of the participants (age, gender, length of work in ICU, education, type of ICU).Results: The majority of the sample were female nursing staff (87.7%), aged 26-35 (38.9%). The results showed that approximately every fifth nurse (22.1%) expressed a high emotional exhaustion (EE), with lesser burden of a high depersonalisation (D) in 7.9%, yet every third nurse (34.5%) scored low on PA. Male nurses reported more depersonalisation (p=0.045), yet neither EE nor the PA dimensions differed by gender.Conclusion: The results of this study concerning burnout are comparable to those of studies of other professions, but the results vary with regard to the sample and the working conditions of the countries.