The purpose of this study was to investigate the association between home gardening and sufficient fruit and vegetable (FV) intake among non-farmers in Thailand, and examine the influence of socio-demographic characteristics and other associated factors on home gardening among non-farmers in urban areas. Data were collected by a cross-sectional survey of a sample of Thai non-farmers (N = 5634). Information on self-reported home gardening, FV intake, health-related behaviors, and socio-demographic characteristics was collected via questionnaire. The findings show that home gardening is significantly associated with sufficient FV intake among non-farmers (p < 0.001). Within the non-farmer group who lived in urban areas, 9% gardened FV at home. Home gardening was significantly associated with socio-demographic characteristics (sex, age and occupation), physical activity, fear of pesticide contamination of FV, and FV safety awareness among the urban non-farmers. Respondents who were female, in the middle-adulthood group, practiced regular physical activity, feared pesticide contamination, and had high awareness of FV safety had the highest probability of gardening at home (p < 0.05, p < 0.001, p < 0.01, p < 0.001 and p < 0.05, respectively). The Thai government should pay more attention to factors that influence urban home gardening by providing support, building local capacity, and implementing effective interventions with the urban population.