Healthy lifestyles are associated with higher levels of perforin, granulysin and granzymes A/B-expressing cells in peripheral blood lymphocytes

Nature Therapy and Preventive Medicine

“…As negative controls, FITC-IgG2b was used for perforin and FITC-IgG1 for GrA/B for 30 min at 4°C according to the manufacturer's instructions. Intracellular GRN was stained with a rabbit anti-human GRN polyclonal antibody using rabbit serum as the negative control after fixation/permeabilization with Cytofix/Cytoperm solution, and then stained with FITC-goat antirabbit IgG for 30 min at 4°C in the dark (Li et al, 2007a(Li et al, , 2007b(Li et al, , 2008a(Li et al, , 2008b(Li et al, , 2010Li, 2010b). After staining, the cells were washed twice with fixative solution and once with PBS containing 1% FBS.…”

“…After staining, the cells were washed twice with fixative solution and once with PBS containing 1% FBS. Flow cytometric analysis was performed with a FACScan flow cytometer as described previously (Li et al, 2007a(Li et al, , 2007b(Li et al, , 2008a(Li et al, , 2008bLi, 2010b). Investigation of human physiological responses in actual field sites is extremely valuable because it can reveal the total effects of the surrounding environment (Lee et al, 2009(Lee et al, , 2011b(Lee et al, , 2011cMatsunaga et al, 2011;Park et al, 2007Park et al, , 2008aPark et al, , 2008bPark et al, , 2009Tsunetsugu et al 2007b).…”

The Development of an Enhanced Extension Agent Training to Create Butterfly Gardens in Louisiana

“…Nearly all the subjects (11 out of 12) showed higher immune system activity after the trip (about a 50 per cent increase) relative to before. The findings of this and subsequent studies by the same research group indicate that a forest bathing trip can have beneficial effects on the body's immune function (Li et al 2008a;Li et al 2007a;Li et al 2007b). The relevance of this research to this report lies in the mediating role of the immune system in the bi-directional relationship between coronary artery disease and depression (Kop and Gottdiener 2005).…”

Association of overtime work with cellular immune markers among healthy daytime white-collar employees

“…Decreased NK cells have been found among people with chronic sleep deprivation, depressive symptoms, and job dissatisfaction (25,26,31). Physical inactivity and smoking have also been reported as factors disturbing NK cells (18,19). Thus it is likely that it is not the overtime work per se that causes a decrease of NK cells but rather a combination of work-related stress, sleep deprivation, depressive symptoms, and poor health behaviors triggered by overtime work.…”

“…Similarly, a study of 291 middleaged male Japanese workers reported that those working ≥12 hours/day (including commuting) had a significant decrease of mitogenic response to phytohemagglutinin-A (PHA) but the levels of interferon (IFN)-γ and interleukin (IL)-4 were comparable to those working <12 hours/day (16). A series of studies assessing the immunological impact of good versus poor health practices (work hours, smoking, drinking, sleeping, exercise, nutrition, stress, and eating breakfast) revealed that working ≥10 hours/ day as compared to <10 hours/day was associated with a significant decrease of NK cell cytotoxicity (NKCC) (17), while it was not associated with lymphokine-activated killer (LAK) cell cytotoxicity or lymphocyte subsets (NK, CD4+ T, CD8+ T, perforin, granulyin, and granzymes A/B-expressing cells) (18,19).…”