Heart and spleen twin grafts in rats. II. Delayed host splenectomy

Tauber, Jürg W.; Blanchard, J

doi:10.1002/micr.1920020407

Microsurgery

1981

DOI: 10.1002/micr.1920020407

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Heart and spleen twin grafts in rats. II. Delayed host splenectomy

Jürg W. Tauber¹,

J Blanchard²

Abstract: Simultaneous auxiliary transplantation of the spleen has been shown to delay and attenuate the rejection of cardiac grafts in rats. In the experiments reported here, 144 such twin grafts in Lewis and ACI rats were explored in an attempt to determine whether removal of the host's spleen would further facilitate graft survival in this model. Thus, 87 rats were submitted to host splenectomy at various time intervals after implantation of the twin grafts. After considerable technical problems were overcome, it was… Show more

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1983

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Cited by 7 publications

References 17 publications

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Heart and spleen “Twin Grafts” in rats: Morphology and function in permanent survivals

1983

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A new rat allograft model has been investigated for its potential to facilitate organ transplantation. In this procedure heart and spleen of male inbred LEW rat (RT1') were connected to the main abdominal vessels in inbred ACI rat (RT1a). With optimal surgical technique we obtain 64% permanent graft survival (5-14 months) in the splenectomized recipients. Cardiac histology ranged from preserved myocardium with moderate thickening of coronary artery walls to diffuse myocardial fibrosis with marked involutional changes of coronary arteries. Whereas palpation and EKG wrongly indicate good function beyond the fourth month, only the phonocardiogram shows the rapid functional loss which reflects the true morphologic involution.

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Heart and spleen “Twin Grafts” in rats: Morphology and function in permanent survivals

1983

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Heart and spleen “twin grafts” in rats: IV. Influence of donor spleen cell mass on allograft survival

et al. 1983

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Heart and spleen twin grafts from LEW to ACI rats may survive permanently, whereas those grafted from ACI to LEW rats do not. This strain difference in graft acceptance was analyzed quantitatively by transplanting one to three ACI spleens into LEW rats in order to compensate for the relatively small size of ACI spleens (Fig. 1). Under these conditions permanent graft survival was not observed in LEW recipients. However, during host splenectomy 3 days after transplantation, a nonimmunological factor was observed. The transplanted ACI spleens showed congestion and infarctions, while in the LEW to ACI transplant model the LEW spleens did not. This observation could be explained by the size of the vascular pedicle in ACI spleens. Although no problem was encountered immediately after completing the anastomoses, allogeneic spleens react by increasing in size, to such an extent that their size exceeds the capacity of the smaller vascular pedicle in ACI rats and results in congestion. Hence, the concept is formulated that early compromise of hemodynamics on a nonimmunological base may determine the fate of ACI spleen graft before immunological factors have a chance to become effective.

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Kidney and spleen twin grafts in rats: Unidirectional graft acceptance in unsuppressed ACI recipients

et al. 1985

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In previous studies, we have shown that vascularized spleen grafts can facilitate the acceptance of simultaneously implanted hearts, especially if host splenectomy is done 3 days later. The present study shows that the same protocol works not only for hearts but also for kidneys. A total of 59 renal allografts were carried out in various combinations between LEW and ACI rats. The recipients were submitted to bilateral nephrectomy at the time of graft implantation and host splenectomy 3 days later. Graft function was assessed by survival time of the animals. In addition, BUN, creatinine, and hematocrit were measured in selected individuals. The following observations were made: 1) LEW kidneys were rejected at 92 +/- 65 days while ACI kidneys failed at 17 +/- 1.4 days (p less than 0.025). 2) Control animals without simultaneous syngenic functioning spleen implant rejected their LEW kidneys at 30.6 +/- 24.3 days (p less than 0.005). 3) Eleven rats never rejected their LEW kidneys (four single, seven twin grafts). 4) BUN, creatinine, and hematocrit differed significantly in rats with functioning kidneys compared to those in end stage renal failure (p less than 0.0005, p less than 0.0025, and p less than 10(-7) respectively). It is concluded that the presence of the donor spleen can facilitate the acceptance of a simultaneous kidney graft to various degrees, depending on the strain combination.

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Heart and spleen twin grafts in rats. II. Delayed host splenectomy

Cited by 7 publications

References 17 publications

Heart and spleen “Twin Grafts” in rats: Morphology and function in permanent survivals

Heart and spleen “Twin Grafts” in rats: Morphology and function in permanent survivals

Heart and spleen “twin grafts” in rats: IV. Influence of donor spleen cell mass on allograft survival

Kidney and spleen twin grafts in rats: Unidirectional graft acceptance in unsuppressed ACI recipients

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