Heart dysfunction induced by choline-deficiency in adult rats: The protective role of l-carnitine

Strilakou, Athina; Lazaris, Andreas C.; Perelas, Apostolos; Mourouzis, Iordanis; Douzis, Ioannis Ch.; Καρκαλούσος, Πέτρος; Stylianaki, Aikaterini; Pantos, Costas; Liapi, Charis

doi:10.1016/j.ejphar.2013.03.025

Cited by 33 publications

(47 citation statements)

References 60 publications

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“…1 Choline has been evidenced as an anti-ROS factor through relieving activation of proinflammatory responses. 32,45,46 PN therapy in the current study significantly decreased the concentrations of plasma choline, phosphocholine, and betaine in infant rats, which were reversed by choline supplementation. These results suggested that a choline-deficient state may be induced by PN.…”

Section: Discussionsupporting

confidence: 45%

“…1,2 Our previous study also found choline supplementation alleviated the oxidative stress and lipid deposit in hepatic C3A cells exposed to excessive energy substrates. 32 Choline deficiency was found to activate cellular apoptosis and cause skeletal muscle injury, and it could protect muscle cells by maintaining the membrane integrity of mitochondria and cells. 1 Choline has been evidenced as an anti-ROS factor through relieving activation of proinflammatory responses.…”

Section: Discussionmentioning

confidence: 99%

“…The supernatants were collected to detect the ROS production using an oxidation-sensitive fluorescent probe dichlorodihydrofluorescein diacetate (Sigma-Aldrich) following the manufacturer's instructions as described previously. 32 The fluorescence intensity was measured at an excitation of 485 nm and emission of 530 nm by a fluorescence spectrophotometer (Hitachi-F-7000; Hitachi, Tokyo, Japan). Protein levels in intestinal sections were also quantified by using the BCA protein assay reagent (Thermo Fisher Scientific, Rockford, IL) according to the manufacturer's instructions.…”

Section: Duodenal Ros Measurementsmentioning

confidence: 99%

See 2 more Smart Citations

Choline Alleviates Parenteral Nutrition–Associated Duodenal Motility Disorder in Infant Rats

Zhu

Guo

et al. 2015

J Parenter Enteral Nutr

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Section: Discussionsupporting

confidence: 45%

Section: Discussionmentioning

confidence: 99%

Section: Duodenal Ros Measurementsmentioning

confidence: 99%

See 1 more Smart Citation

Choline Alleviates Parenteral Nutrition–Associated Duodenal Motility Disorder in Infant Rats

Zhu

Guo

et al. 2015

J Parenter Enteral Nutr

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“…In the present experimental setting, we have already published data concerning the degree of cardiac interstitial fibrosis , according to which the performed modified Masson's Trichrome staining in the examined specimens revealed a significant fibrosis in the choline‐deficient myocardial tissue . The assessment of the extent of cardiac fibrosis was conducted in a way similar to the one that is usually used in such histopathologic studies .…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical determination of the extracellular matrix modulation in a rat model of choline‐deprived myocardium: the effects of carnitine

Strilakou

Perelas

Lazaris

et al. 2015

Fundamemntal Clinical Pharma

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Choline has been identified as an essential nutrient with crucial role in many vital biological functions. Recent studies have demonstrated that heart dysfunction can develop in the setting of choline deprivation even in the absence of underlying heart disease. Matrix metalloproteinases (MMPs) are responsible for extracellular matrix degradation, and the dysregulation of MMP-2 and MMP-9 has been involved in the pathogenesis of various cardiovascular disorders. The aim of the study was to investigate the role of MMPs and their inhibitors (TIMPs), in the pathogenesis of choline deficiency-induced cardiomyopathy, and the way they are affected by carnitine supplementation. Male Wistar Albino adult rats were divided into four groups and received standard or choline-deficient diet with or without L-carnitine in drinking water (0.15% w/v) for 1 month. Heart tissue immunohistochemistry for MMP-2, MMP-9, TIMP-1, and TIMP-2 was performed. Choline deficiency was associated with suppressed immunohistochemical expression of MMP-2 and an increased expression of TIMP-2 compared to control, while it had no impact on TIMP-1. MMP-9 expression was decreased without, however, reaching statistical significance. Carnitine did not affect MMP-2, MMP-9, TIMP-1 or TIMP-2 expression. The pattern of TIMP and MMP modulation observed in a choline deficiency setting appears to promote fibrosis. Carnitine, although shown to suppress fibrosis, does not seem to affect MMP-2, MMP-9, TIMP-1 or TIMP-2 expression. Further studies will be required to identify the mechanism underlying the beneficial effects of carnitine.

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“…L-carnitine also blocks dietary vitamin B deficiency-induced left ventricular dysfunction and attenuates the diet-caused increase in serum BNP [23]. In patients with chronic heart failure, the plasma carnitine levels are correlated with %FS, EF, and LVMI [24].…”

Section: Effects On Cardiac Parametersmentioning

confidence: 99%

Effects of L-carnitine supplementation on nutritional, immunological, and cardiac parameters in hemodialysis patients: a pilot study

et al. 2015

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Background: L-carnitine is an essential compound that facilitates the transport of long-chain fatty acid across the inner mitochondrial membrane for β-oxidation. However, the effect of L-carnitine supplementation remains to be fully explored in patients with chronic kidney disease. We aimed in this study to determine the multidirectional effects of L-carnitine supplementation on clinical parameters in more detail. Methods: We orally administered L-carnitine to maintain serum-free carnitine levels within the normal range (30 to 70 μmol/L) for 6 months in 21 hemodialysis (HD) patients (age, 74 ± 11 years; time on HD, 60 ± 84 months).

show abstract

Heart dysfunction induced by choline-deficiency in adult rats: The protective role of l-carnitine

Cited by 33 publications

References 60 publications

Choline Alleviates Parenteral Nutrition–Associated Duodenal Motility Disorder in Infant Rats

Choline Alleviates Parenteral Nutrition–Associated Duodenal Motility Disorder in Infant Rats

Immunohistochemical determination of the extracellular matrix modulation in a rat model of choline‐deprived myocardium: the effects of carnitine

Effects of L-carnitine supplementation on nutritional, immunological, and cardiac parameters in hemodialysis patients: a pilot study

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