Heart-on-a-chip systems with tissue-specific functionalities for physiological, pathological, and pharmacological studies

Gu, Bingsong; Han, Kang; Cao, Hanbo; Huang, Xinxin; Li, Xiao; Mao, Mao; Zhu, Hui; Cai, Hu; Li, Dichen; He, Jiankang

doi:10.1016/j.mtbio.2023.100914

Materials Today Bio

2024

DOI: 10.1016/j.mtbio.2023.100914

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Heart-on-a-chip systems with tissue-specific functionalities for physiological, pathological, and pharmacological studies

Bingsong Gu,

Kang Han,

Hanbo Cao

et al.

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2024

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Cited by 2 publications

References 153 publications

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Straddling the Line Between In Vitro and Ex Vivo Investigations

Klein,

Hutmacher

2024

Tissue Engineering Part C: Methods

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Straddling the Line Between In Vitro and Ex Vivo Investigations

Klein,

Hutmacher

2024

Tissue Engineering Part C: Methods

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Cell cycle visualization tools to study cardiomyocyte proliferation in real-time

Salmenov,

Mummery,

ter Huurne

2024

Open Biol.

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Cardiomyocytes in the adult human heart are quiescent and those lost following heart injury are not replaced by proliferating survivors. Considerable effort has been made to understand the mechanisms underlying cardiomyocyte cell cycle exit and re-entry, with view to discovering therapeutics that could stimulate cardiomyocyte proliferation and heart regeneration. The advent of large compound libraries and robotic liquid handling platforms has enabled the screening of thousands of conditions in a single experiment but success of these screens depends on the appropriateness and quality of the model used. Quantification of (human) cardiomyocyte proliferation in high throughput has remained problematic because conventional antibody-based staining is costly, technically challenging and does not discriminate between cardiomyocyte division and failure in karyokinesis or cytokinesis. Live cell imaging has provided alternatives that facilitate high-throughput screening but these have other limitations. Here, we (i) review the cell cycle features of cardiomyocytes, (ii) discuss various cell cycle fluorescent reporter systems, and (iii) speculate on what could improve their predictive value in the context of cardiomyocyte proliferation. Finally, we consider how these new methods can be used in combination with state-of-the-art three-dimensional human cardiac organoid platforms to identify pro-proliferative signalling pathways that could stimulate regeneration of the human heart.

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Heart-on-a-chip systems with tissue-specific functionalities for physiological, pathological, and pharmacological studies

Cited by 2 publications

References 153 publications

Straddling the Line Between In Vitro and Ex Vivo Investigations

Straddling the Line Between In Vitro and Ex Vivo Investigations

Cell cycle visualization tools to study cardiomyocyte proliferation in real-time

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