Heart valve bioprothesis; effect of different acellularizations methods on the biomechanical and morphological properties of porcine aortic and pulmonary valve

Wilczek, Piotr

doi:10.2478/v10175-010-0032-4

Cited by 5 publications

(6 citation statements)

References 14 publications

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“…Not with standing, decellularized pulmonary valves such as the CryoValve SG pulmonary human valve have been previously cleared and are currently being studied prospectively to determine mortality, reoperation, structural deterioration, thromboembolism, conduit failure, calcification among other adverse events [56][57][58]. Replacement TEPVs developed from biodegradable scaffolds seeded with autologous cells have proven successful in large animal and human models [29,31,[59][60][61]. From a fabrication stand point, the scaffold design is critical; the 3D structure guides cellular migration, attachment and growth [62][63][64].…”

Section: In Vitro Modeling and Fabricationmentioning

confidence: 99%

“…The ideal scaffold should be optimally biodegradable-the timing of absorption into endogenous tissue should match tissue formation. Scaffolds have been developed from polyglycolic acid (PGA), polylactic acid (PLA), combined PGA-PLA and polyhydroxyoctanoate (PHO) [29,[59][60][61][62][63][64][65]. The advantage with these materials is thermoplasticity, biodegradability and biocompatibility [29,[60][61][62][63][64][65].…”

Section: In Vitro Modeling and Fabricationmentioning

confidence: 99%

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Bone marrow mesenchymal stem cells for tissue engineered pulmonary valves (TEPV)

Asumda¹,

Lamin²

2014

J Regen Med Tissue Eng

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Heart valve tissue engineering and regeneration represents a novel, and viable alternative treatment modality for valvular heart disease. Tissue-engineered pulmonary valves (TEPV) have been constructed from autologous cells grown on 3D biodegradable scaffolds or decelluarized xeno-or homografts and surgically implanted in animal models. From a translational stand point, constructed valves must maintain regenerative competency in recipients, with minimum risk for stenosis over the long term. Stem cells are an attractive cell source for this proposed solution; candidate cells are expected to maintain robust differentiation and transdifferentiation capacity. Transplanted cells must persist, engraft, and couple electro-mechanically with endogenous tissue. Here, we present a discussion on the clinical applicability of bone marrow mesenchymal stem cells in TEPV.

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Section: In Vitro Modeling and Fabricationmentioning

confidence: 99%

Section: In Vitro Modeling and Fabricationmentioning

confidence: 99%

Bone marrow mesenchymal stem cells for tissue engineered pulmonary valves (TEPV)

Asumda¹,

Lamin²

2014

J Regen Med Tissue Eng

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“…The tissue biomechanics may depend on various factors, such as the age of the donor and tissue source [ 16 , 17 ]. In the case of tissue-engineering techniques, the acellularization method can also affect the biomechanics and morphology of the tissue [ 18 ]. The storage conditions of the scaffolds could also be an important factor that may affect the mechanical stability and morphology of the bioprosthetic heart valves.…”

Section: Introductionmentioning

confidence: 99%

Biomechanical and morphological stability of acellular scaffolds for tissue-engineered heart valves depends on different storage conditions

Wilczek¹,

Paulina²,

Karolina³

et al. 2018

J Mater Sci: Mater Med

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Currently available bioprosthetic heart valves have been successfully used clinically; however, they have several limitations. Alternatively, tissue-engineering techniques can be used. However, there are limited data concerning the impact of storage conditions of scaffolds on their biomechanics and morphology. The aim of this study was to determine the effect of different storage conditions on the biomechanics and morphology of pulmonary valve dedicated for the acellular scaffold preparation to achieve optimal conditions to obtain stable heart valve prostheses. Scaffold can then be used for the construction of tissue-engineered heart valve, for this reason evaluation of these parameters can determine the success of the clinical application this type of bioprosthesis. Pulmonary heart valves were collected from adult porcines. Materials were divided into five groups depending on the storage conditions. Biomechanical tests were performed, both the static tensile test, and examination of viscoelastic properties. Extracellular matrix morphology was evaluated using transmission electron microscopy and immunohistochemistry. Tissue stored at 4 °C exhibited a higher modulus of elasticity than the control (native) and fresh acellular, which indicated the stiffening of the tissue and changes of the viscoelastic properties. Such changes were not observed in the radial direction. Percent strain was not significantly different in the study groups. The storage conditions affected the acellularization efficiency and tissue morphology. To the best of our knowledge, this study is the first that attributes the mechanical properties of pulmonary valve tissue to the biomechanical changes in the collagen network due to different storage conditions. Storage conditions of scaffolds for tissue-engineered heart valves may have a significant impact on the haemodynamic and clinical effects of the used bioprostheses.

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“…In the course of clinical studies many investigators have assumed the PAC technique as a reference Literature studies and the authors' practical experiences have shown that the accuracy of the thermodilution method depends on many factors (e.g. physical parameters of the indicator, haematological parameters of blood, heart rate, valves diseases [20] and on the way of injecting of the indicator [9,10]) Manufacturers of the patient's monitor devices quoted, that the unreliability of CO measurements by means of thermodilution amounts to about 5% [11,12]. However this value contradicts clinical observations.…”

Section: Introductionmentioning

confidence: 99%

Analysis of actual accuracy in cardiac output measurements by means of thermodilution

Gawlikowski¹,

Pustelny²

2012

Bulletin of the Polish Academy of Sciences: Technical Sciences

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Abstract. The essential examination in hemodynamic monitoring of the patient is the cardiac output (CO) measurement. Nowadays, in clinical practice the most popular method is indicator dilution, particularly thermodilution. It is realized by applying the Swan-Ganz catheter and observing changes of the indicator concentration. This method is sensitive to many factors and the obtained results should be treated cautiously. The paper presents theoretical and experimental studies of metrological phenomena in CO measurements by means of thermodilution, paying special attention to medical aspects of the measurements. It has been pointed out that the actual unreliability of the mentioned method reaches values from 20% to 45%, which is in opposition to the technical data of patient's monitors (typical accuracy about 5%).

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Heart valve bioprothesis; effect of different acellularizations methods on the biomechanical and morphological properties of porcine aortic and pulmonary valve

Cited by 5 publications

References 14 publications

Bone marrow mesenchymal stem cells for tissue engineered pulmonary valves (TEPV)

Bone marrow mesenchymal stem cells for tissue engineered pulmonary valves (TEPV)

Biomechanical and morphological stability of acellular scaffolds for tissue-engineered heart valves depends on different storage conditions

Analysis of actual accuracy in cardiac output measurements by means of thermodilution

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