Heartwater<i>(Cowdria ruminantium</i>infection) on São Tomé

Uilenberg, Gerrit; Corten, J. J. F. M.; Dwinger, R.H.

doi:10.1080/01652176.1982.9693849

Cited by 30 publications

(40 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The disease affects domestic and wild ruminants and occurs throughout subSaharan Africa and also on some islands in the Caribbean (Uilenberg, 1983). The organism is transstadially transmitted by ticks of the genus Amblyomma .…”

Section: Introductionmentioning

confidence: 99%

Differential transcription of the major antigenic protein 1 multigene family of Ehrlichia ruminantium in Amblyomma variegatum ticks

Postigo

Taoufik

Bell‐Sakyi

et al. 2007

Veterinary Microbiology

View full text Add to dashboard Cite

The rickettsial pathogen Ehrlichia ruminantium causes heartwater in ruminants and is transmitted by ticks of the genus Amblyomma. The map1 gene, encoding the major surface protein MAP1, is a member of a multigene family containing 16 paralogs. In order to investigate differential transcription of genes of the map1 multigene family in vivo in unfed and feeding ticks, RNA was extracted from midguts and salivary glands of E. ruminantium-infected adult female Amblyomma variegatum ticks and analysed by RT-PCR using MAP1 paralog-specific primers. In unfed ticks, only transcripts from the map1-1 gene were observed in midguts and no transcripts were detected in salivary glands. In feeding ticks, map1-1 transcripts were more abundant in midguts whereas high levels of map1 transcripts were observed in salivary glands. Our results show that differential transcription of genes of the E. ruminantium map1 cluster occurs in vivo in different tissues of infected ticks before and during transmission feeding, indicating that openUP this multigene family may be involved in functions of biological relevance in different stages of the life cycle of E. ruminantium.

show abstract

Section: Introductionmentioning

confidence: 99%

Differential transcription of the major antigenic protein 1 multigene family of Ehrlichia ruminantium in Amblyomma variegatum ticks

Postigo

Taoufik

Bell‐Sakyi

et al. 2007

Veterinary Microbiology

View full text Add to dashboard Cite

show abstract

“…Economic losses due to heartwater are significant due to the high mortality rates, which range from 20 to 90 % (Uilenberg, 1983(Uilenberg, , 1990. Losses due to morbidity are likel!.…”

Section: Introductionmentioning

confidence: 99%

“…Heartwater or cowdriosis is an infectious, economically important disease of livestock which is caused by the Gram-negative rickettsia Cowdria ruminantium and transmitted by ticks of the genus Ambbomma (Uilenberg, 1983). Economic losses due to heartwater are significant due to the high mortality rates, which range from 20 to 90 % (Uilenberg, 1983(Uilenberg, , 1990.…”

Section: Introductionmentioning

confidence: 99%

“…The traditional methods of diagnosis of heartwater include: (a) recognition of clinical signs, which are not unique to heartwater and can be confused with other diseases (Uilenberg, 1983) ; (b) microscopic examination of brain biopsies (Purchase, 1945), which is impractical for field use; and (c) subinoculation of blood into susceptible animals (Uilenberg, 1983), which is cumbersome and expensive. Improved methods for diagnosis of heartwater by utilization of nucleic-acid-based assays are being investigated (Waghela e t al., …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular cloning of a gene encoding the immunogenic 21 kDa protein of Cowdria ruminantium

Mahan

McGuire

Semu³

et al. 1994

Microbiology

View full text Add to dashboard Cite

“…C. ruminantium is transmitted by ticks of the genus Amblyomma and causes heartwater in domestic ruminants throughout sub-Saharan Africa (Uilenberg, 1983 ;Jongejan & Bekker, 1999), where it is a serious constraint to animal production. The disease is also present in the Caribbean region, where it poses the threat of spreading to the American mainland (Perreau et al, 1980 ;Barre!…”

Section: Introductionmentioning

confidence: 99%

Genome size and genetic map of Cowdria ruminantium

et al. 2000

View full text Add to dashboard Cite

Cowdria ruminantium is the cause of a serious tick-borne disease of domestic ruminants, known as heartwater or cowdriosis. The organism belongs to the tribe Ehrlichieae, which contains obligate intracellular pathogens, causing several important animal and human diseases. Although a few C. ruminantium genes have been cloned and sequenced, very little is known about the size, gross structure and organization of the genome. This paper presents a complete physical map and a preliminary genetic map for C. ruminantium. Chromosomal C. ruminantium DNA was examined by PFGE and Southern hybridization. PFGE analysis revealed that C. ruminantium has a circular chromosome approximately 1576 kb in size. A physical map was derived by combining the results of PFGE analysis of DNA fragments resulting from digestion of the whole genome with KspI, RsrII and SmaI and Southern hybridization analysis with a series of gene probes and isolated macrorestriction fragments. A genetic map for C. ruminantium with a mean resolution of 290 kb was established, the first for a member of the Ehrlichieae. A total of nine genes or cloned C. ruminantium DNA fragments were mapped to specific KspI, RsrII and SmaI fragments, including the major antigenic protein gene, map-1.

show abstract

Heartwater(Cowdria ruminantiuminfection) on São Tomé

Cited by 30 publications

References 1 publication

Differential transcription of the major antigenic protein 1 multigene family of Ehrlichia ruminantium in Amblyomma variegatum ticks

Differential transcription of the major antigenic protein 1 multigene family of Ehrlichia ruminantium in Amblyomma variegatum ticks

Molecular cloning of a gene encoding the immunogenic 21 kDa protein of Cowdria ruminantium

Genome size and genetic map of Cowdria ruminantium

Contact Info

Product

Resources

About