Heartwood Extract of Pterocarpus marsupium Roxb. Offers Defense against Oxyradicals and Improves Glucose Uptake in HepG2 Cells

Abstract

Diabetes mellitus leads to cellular damage and causes apoptosis by oxidative stress. Heartwood extract of Pterocarpus marsupium has been used in Ayurveda to treat various diseases such as leprosy, diabetes, asthma, and bronchitis. In this study, we worked out the mechanism of the antidiabetic potential of methanolic heartwood extract of Pterocarpus marsupium (MPME). First, metabolic profiling of MPME was done using gas chromatography-mass spectrometry (GCMS), ultra-performance liquid chromatography-mass spectroscopy (UPLC-MS), and high-performance thin-layer chromatography (HPTLC) to identify phenols, flavonoids, and terpenoids in MPME. Biological studies were carried out in vitro using the HepG2 cell line. Many antidiabetic compounds were identified including Quercetin. Methanolic extract of MPME (23.43 µg/mL–93.75 µg/mL) was found to be safe and effective in reducing oxyradicals in HepG2 cells. A concentration of 93.75 µg/mL improved glucose uptake efficiently. A significant decrease in oxidative stress, cell damage, and apoptosis was found in MPME-treated HepG2 cells. The study suggests that the heartwood of Pterocarpus marsupium offers good defense in HepG2 cells against oxidative stress and improves glucose uptake. The results show the significant antidiabetic potential of MPME using a HepG2 cell model. The effect seems to occur by reducing oxidative stress and sensitizing the cells towards glucose uptake, hence lowering systemic glucose levels, as well as rescuing ROS generation.

In Silico Prediction of Antidiabetic Activity of Phytoconstituents of Pterocarpus Marsupium Targeting α-Amylase Enzyme

“…Additionally, several phytoconstituents such flavonoids, terpenoids, cardiac glycosides, alkaloids, glycosides, saponins, tannins, proteins, and carbohydrates in ethanol, acetone, and isopropyl alcohol (IPA) (1:1) extracts of Pterocarpus marsupium stem wood. 19 This investigation supports our in silico study that pterostilbenes and liquirtigenin have the lowest binding energy (-8.1 kcal/mol) with complex á-amylase to reduce blood sugar levels. Hence, it is manifest that our study screened phytoconstituents have binding energy, docking score, and interaction with catalytic residue, leading to blocking the action of á-amylase to restrict diabetes.…”

Inhibition of Autophagy and the Cytoprotective Role of Smac Mimetic against ROS-Induced Cancer: A Potential Therapeutic Strategy in Relapse and Chemoresistance Cases in Breast Cancer

“…A flow cytometer (BD, Aria, Lenoir, NC, USA) was also utilized to detect the DCF fluorescence at excitation 488 nm and emission 525 nm, respectively. In addition, we incubated the cells for 2 h with ascorbic acid, a strong antioxidant [22].…”