This communication investigates the simultaneous impact of the magnetic field, heat absorption and mixed convection in electroosmotically induced peristaltic flow of copper particles in the blood. The two-phase flow model is used in the microchannel with flexible walls. The thermal features of nanofluids are studied using copper nanoparticles. The Poisson-Boltzmann equation is employed to accommodate the electrical double layer in the microchannel. With Debye-Hückel, lubrication and long wavelength approximations, the problem becomes non-dimensional. Exact solutions have been calculated for velocity, stream function, pressure rise, pressure gradient and heat transfer coefficient and temperature profile. Impact of different embedded parameters on flow properties is graphically illustrated. It is found that the electroosmotic parameter strongly effects the velocity profile and bolus formation. Also, the decreasing behavior of temperature is presented, which clarifies the nanofluid as a cooling agent. The results show an increment in peristaltic pumping with rise in mixed convection while it decreases with magnetic field. Furthermore, a comparative study of pure blood and copper blood is conducted. The obtained results may be suitable in the peristaltic pump modulation for the efficient operation of a variety of industrial, biomedical and drug delivery systems in the microfluidic device.