Heat and radiofrequency plasma glow discharge pretreatment of a titanium alloy: Eveidence for enhanced osteoinductive properties

Rapuano, Bruce E.; Singh, Herman; Boskey, Adele L.; Doty, Stephen B.; MacDonald, Daniel E.

doi:10.1002/jcb.24536

Cited by 12 publications

(5 citation statements)

References 46 publications

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“…Previous studies have shown that materials, such as Ti-6Al-4V, can be utilized to manufacture implants or prostheses [ 6 , 7 ]. Cellular biomaterials fabricated using EBM have been widely used due to their good osteoinductive properties [ 8 ] and mechanical properties, as demonstrated in researches of bone tissue engineering studies. Relative tests have been conducted to measure their stiffness, and the results suggest that the mechanic behavior of the biomaterials in the cellular microstructure is similar to a cancellous bone [ 9 ]; thus, it is suitable for printing implants or prostheses using EBM for clinical applications.…”

Section: Introductionmentioning

confidence: 99%

A study on the mechanical characteristics of the EBM-printed Ti-6Al-4V LCP plates in vitro

et al. 2014

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PurposeThe electron beam melting (EBM) Ti-6Al-4V material technology has been developed over a short time period. It was introduced through a research to develop Ti-6Al-4V implants for patients, but EBM printed locking compression plates have not been used for clinical implants. The main purpose of this study is to find whether the EBM Ti-6Al-4V plate suit for clinical implants.MethodsFirst, we scanned an AO-locking compression plate (LCP) and printed LCP samples using EBM. Next, we evaluated the EBM plate surface roughness through optical microscopy as well as the LCP and EBM plates’ mechanical characteristics using the ASTM standard, which is commonly used to test the mechanical properties of bone plates subject to bending. Each sample was examined using a single-cycle four-point bending test and hardness testing to acquire data on bending stiffness, bending strength, bending structural stiffness, and hardness.ResultsThe results show significant differences in bending stiffness, bending strength, bending structural stiffness, and hardness between the samples using EBM and the original LCP plates. The EBM-printed samples’ surface roughness was 0.49 ± 0.02 μm. The mean hardness of the LCP sample was 266.67 HV10 ± 5.8, and the EBM-printed sample mean hardness was 341.1 HV10 ± 1.93. The EBM samples’ bending stiffness was 87.67%, which is greater than using the LCP plates’; and the bending strength was 190.7% greater, the bending structural stiffness was 73.2% greater, and the hardness was 27.9% greater.ConclusionsThe results show that the EBM plates’ general mechanical strength was significantly greater than the LCP plates. An EBM plate is advantageous for clinical implants because it can be customized with great potential for improvement.

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Section: Introductionmentioning

confidence: 99%

A study on the mechanical characteristics of the EBM-printed Ti-6Al-4V LCP plates in vitro

et al. 2014

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“…This supposition is supported by FTIR findings showing areas of new trabecular bone formation near fibronectincoated implants signified by higher acid phosphate levels and lower mineral:matrix ratios [Spevak et al, 2013] compared to the surrounding trabecular or cortical bone. Moreover, we have shown that the pretreatments enhance matrix gene/osteoblast marker gene expression [Rapuano et al, 2012c], cell-directed mineralization [Rapuano et al, 2013], and trabecular bonding/implant shear strength even in the absence of an exogenous fibronectin coating. These latter results suggest that the bioactivities of other endogenous osteogenic proteins that adsorb to the implant surface in vivo are also increased by our pretreatments resulting in downstream effects on osseointegration.…”

Section: The Role Of Fibronectin In the Effects Of Rfgd And Heat Pretmentioning

confidence: 93%

“…The differences between the two pretreatments of in their effects on osteoblast marker gene expression [Rapuano et al, 2012c], celldirected mineralization [Rapuano et al, 2013], and osseointegration may have arisen from the unique physicochemical characteristics of each resultant oxide surface. We found that heat and RFGD pretreatments increased the Ti6Al4V oxide 0 s net surface charge with the latter pretreatment exerting a greater effect .…”

Section: Effects Of Ti6al4v Oxide Physical and Chemical Properties Onmentioning

confidence: 99%

“…In a more recent study, we showed that these same pretreatments of Ti6Al4V stimulated bone mineral formation in cultures of attached MC3T3 osteoprogenitor cells in vitro [Rapuano et al, 2013]. To determine if the pretreatments of the alloy also affected its osseointegration in vivo, a rat model was used to measure new bone formation and implant-bone bonding strength.…”

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confidence: 99%

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Heat and radiofrequency plasma glow discharge pretreatment of a titanium alloy promote bone formation and osseointegration

et al. 2013

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Orthopedic and dental implants manifest increased failure rates when inserted into low density bone. We determined whether chemical pretreatments of a titanium alloy implant material stimulated new bone formation to increase osseointegration in vivo in trabecular bone using a rat model. Titanium alloy rods were untreated or pretreated with heat (600°C) or radiofrequency plasma glow discharge (RFGD). The rods were then coated with the extracellular matrix protein fibronectin (1 nM) or left uncoated and surgically implanted into the rat femoral medullary cavity. Animals were euthanized 3 or 6 weeks later, and femurs were removed for analysis. The number of trabeculae in contact with the implant surface, surface contact between trabeculae and the implant, and the length and area of bone attached to the implant were measured by histomorphometry. Implant shear strength was measured by a pull-out test. Both pretreatments and fibronectin enhanced the number of trabeculae bonding with the implant and trabeculae-to-implant surface contact, with greater effects of fibronectin observed with pretreated compared to untreated implants. RFGD pretreatment modestly increased implant shear strength, which was highly correlated (r2 = 0.87 – 0.99) with measures of trabecular bonding for untreated and RFGD-pretreated implants. In contrast, heat pretreatment increased shear strength 3 to 5-fold for both uncoated and fibronectin-coated implants at 3 and 6 weeks, suggesting a more rapid increase in implant-femur bonding compared to the other groups. In summary, our findings suggest that the heat and RFGD pretreatments can promote the osseointegration of a titanium alloy implant material.

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“…Modification focuses upon the interaction of implant to body fluid which helps in bone healing. Plasma increases surface roughness and wettability which help in cell adhesion [ 83 , 84 ]. Chairside application of non-thermal atmospheric pressure plasma (NTAPP) before placement of the implant is recently reported, which helps in reducing contact angle and supporting the spread of osteoblastic cells [ 85 , 86 ].…”

Section: Application Of Ntp In Dentistrymentioning

confidence: 99%

Aurora Borealis in dentistry: The applications of cold plasma in biomedicine

Lata

Chakravorty

Mitra

et al. 2022

Materials Today Bio

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Plasma is regularly alluded to as the fourth form of matter. Its bounty presence in nature along with its potential antibacterial properties has made it a widely utilized disinfectant in clinical sciences. Thermal plasma and non-thermal (or cold atmospheric) plasma (NTP) are two types of plasma. Atoms and heavy particles are both available at the same temperature in thermal plasma. Cold atmospheric plasma (CAP) is intended to be non-thermal since its electrons are hotter than the heavier particles at ambient temperature. Direct barrier discharge (DBD), atmospheric plasma pressure jet (APPJ), etc. methods can be used to produce plasma, however, all follow a basic concept in their generation. This review focuses on the anticipated uses of cold atmospheric plasma in dentistry, such as its effectiveness in sterilizing dental instruments by eradicating bacteria, its advantage in dental cavity decontamination over conventional methods, root canal disinfection, its effects on tooth whitening, the benefits of plasma treatment on the success of dental implant placement, and so forth. Moreover, the limitations and probable solutions has also been anticipated. These conceivable outcomes thus have proclaimed the improvement of more up-to-date gadgets, for example, the plasma needle and plasma pen, which are efficient in treating the small areas like root canal bleaching, biofilm disruption, requiring treatment in dentistry.

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Heat and radiofrequency plasma glow discharge pretreatment of a titanium alloy: Eveidence for enhanced osteoinductive properties

Cited by 12 publications

References 46 publications

A study on the mechanical characteristics of the EBM-printed Ti-6Al-4V LCP plates in vitro

A study on the mechanical characteristics of the EBM-printed Ti-6Al-4V LCP plates in vitro

Heat and radiofrequency plasma glow discharge pretreatment of a titanium alloy promote bone formation and osseointegration

Aurora Borealis in dentistry: The applications of cold plasma in biomedicine

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