Heat-induced changes in respiratory pathways and mitochondrial structure during microcycle conidiation of Neurospora crassa

Michéa-Hamzehpour, M.; Grange, F.; That, T. Ton; Turian, G.

doi:10.1007/bf00403197

Cited by 17 publications

(6 citation statements)

References 13 publications

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“…S. cerevisiae grown on the nonfermentable substrate acetate continued to synthesize heat shock proteins indefinitely at the high temperature (28). Michea-Hamzehpour et al (34) have reported that heat-treated N. crassa conidia utilize an alternate pathway of respiration that is inhibited by salicylhydroxamic acid but not by cyanide (23), and we have confirmed this in our laboratory (data not shown). It is possible that the ability of N. crassa to switch from the normal cyanide-sensitive pathway of respiration to the alternate, noncytochrome-mediated pathway is related to the transiency of its heat shock response.…”

Section: Discussionsupporting

confidence: 86%

Heat shock response of Neurospora crassa: protein synthesis and induced thermotolerance

Plesofsky-Vig¹,

Brambl²

1985

J Bacteriol

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At elevated temperatures, germinating conidiospores of Neurospora crassa discontinue synthesis of most proteins and initiate synthesis of three dominant heat shock proteins of 98,000, 83,000, and 67,000 Mr and one minor heat shock protein of 30,000 Mr. Postemergent spores produce, in addition to these, a fourth major heat shock protein of 38,000 Mr and a minor heat shock protein of 34,000 Mr. TheK three heat shock proteins of lower

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Section: Discussionsupporting

confidence: 86%

Heat shock response of Neurospora crassa: protein synthesis and induced thermotolerance

Plesofsky-Vig¹,

Brambl²

1985

J Bacteriol

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“…For example, in Sclerotinia sclerotiorum, AOX participates in the regulation of growth, development, and responses to oxidative stress (48). In Aspergillus fumigatus and Neurospora crassa, cyanideinsensitive respiration catalyzed by AOX is induced by heat shock and oxidative stress (49,50). This study showed that the resistance of OE-aox strains to heat stress was significantly stronger than that of the WT strain.…”

Section: Discussionmentioning

confidence: 74%

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Hou

Zhao

Huang

et al. 2020

Appl Environ Microbiol

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Pleurotus ostreatus is widely cultivated in China. However, its cultivation is strongly affected by seasonal temperature changes, especially the high temperatures of summer. Nitric oxide (NO) was previously reported to alleviate oxidative damage to mycelia by regulating trehalose. In this study, we found that NO alleviated oxidative damage to P. ostreatus mycelia by inhibiting the protein and gene expression of aconitase (ACO), and additional studies found that the overexpression and interference of aco could affect the content of citric acid (CA). Furthermore, the addition of exogenous CA can induce alternative oxidase (aox) gene expression under heat stress, reduce the content of H 2 O 2 in mycelium, and consequently protect the mycelia under heat stress. An additional analysis focused on the function of the aox gene in the heat stress response of mycelia. The results show that the colony diameter of the aox overexpression (OE-aox) strains was significantly larger than that of the wild-type (WT) strain under heat stress (32°C). In addition, the mycelia of OEaox strains showed significantly enhanced tolerance to H 2 O 2 . In conclusion, this study demonstrates that NO can affect CA accumulation by regulating aco gene and ACO protein expression and that CA can induce aox gene expression and thereby be a response to heat stress. IMPORTANCE Heat stress is one of the abiotic stresses that affect the growth and development of edible fungi. Our previous study found that exogenous NO had a protective effect on mycelia under heat stress. However, its regulatory mechanism had not been elucidated. In this study, we found that NO altered the respiratory pathway of mycelia under heat stress by regulating aco. The results have enhanced our understanding of NO signaling pathways in P. ostreatus.

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“…Under these new conditions, we obtained a greater proportion of mitochondria with a density of 1 195 g ml-l, similar to that of mycelial mitochondria (Michea-Hamzehpour et al, 1979), than with a density of 1-212g ml-l, previously considered as characteristic of conidia (Ortega Perez et al, 1977). This mitochondrial heterogeneity might arise directly from the increased recovery and improved preservation of the mitochondria.…”

Section: Discussionmentioning

confidence: 77%

“…They were also clearly distinguishable from those isolated from mycelia (Michea-Hamzehpour et al, 1979).…”

Section: Discussionmentioning

confidence: 93%

Respiratory Properties of Mitochondria Isolated from Conidia of Neurospora crassa

Pérez¹,

Michéa-Hamzehpour²,

Turian

1981

Microbiology

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Mitochondria from conidia of Neurospora crassa were isolated and purified by a simple and rapid technique. Electron micrographic analysis and isopycnic centrifugation on a sucrose density gradient revealed a heterogeneous pbpulation.Succinate, citrate, 2-oxoglutarate, NADH and ascorbate + tetramethyl-pphenylenediamine (TMPD) were oxidized by the mitochondria. Oxygen consumption was totally inhibited by antimycin A or cyanide. The electron transport chain was coupled to sites 1 and 2 of phosphorylation. Oligomycin, atractyloside and 2,4-dinitrophenol prevented energy coupling. I N T R O D U C T I O N Respiratory properties of mitochondria isolated from mycelia at different growth stages of Neurospora crassa wild type are now well characterized (Hall '8z Greenawalt, 1967; Weiss et al., 1970; LamboWitz et al., 1972a, b; von Jagow et al., 1973).It has been reported that conidial mitochondria of N. crassa have a low rate of oxygen consumption and that this respiration is predominantly cyanide insensitive (Colvin et al., 1973) and fails to perform oxidative phosphorylation (Greenawalt et al., 1972). However, in a preliminary study, Hall 8z Greenawalt (1964) showed that mitochondria extracted from conidia of N. crassa were able to carry out oxidative phosphorylation. To clarify this discrepancy, we have developed an improved procedure for the extraction of conidial mitochondria of N. crassa (Ortega Perez et al., 1977) which enables us to isolate large quantities of relatively pure mitochondria which show structural integrity -and well-preserved respiratory functions. After extraction under these improved-conditions, conidial mitochondria possess a functional electron transport chain which is partially coupled to oxidative phosphorylation and totally sensitive to cyanide and antimycin A. Heterogeneity of mitochondria was observed by electron microscopy and isopycnic centrifugation. M E T H O D SOrganism and cultural conditions. The wild type of Neurospora crassa strain STA, 262A was obtained from the Fungal Genetic Stock Center, Humboldt State University, Arcata, California, U S A . Conidia were produced by inoculating 1.8 1 Fernbach flasks containing 200 ml Neurospora Minimal Medium (Difco) and 2% (w/v) agar with 2 x lo* conidia. Germination and growth proceeded for 2 d in the dark at 25 OC; the cultures were then kept for 3 d at the same temperature under continuous fluorescent light and aerated with humidified air.Cell disruption and mitochondria1 preparation. Conidia of N. crassa were harvested with distilled water, filtered through four layers of gauze and concentrated by centrifugation at 2 500 g for 10 min. The conidial pellet was further washed with distilled water and reconcentrated by low-sped centrifugation. Conidia were disrupted on a Vortex mixer modified as follows: the rubber ring was replaced by a disc of PVC (3 mm thick) with eight 0022-1287/81/0000-9864 $02.00 0 1981 SGM

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Heat-induced changes in respiratory pathways and mitochondrial structure during microcycle conidiation of Neurospora crassa

Cited by 17 publications

References 13 publications

Heat shock response of Neurospora crassa: protein synthesis and induced thermotolerance

Heat shock response of Neurospora crassa: protein synthesis and induced thermotolerance

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Respiratory Properties of Mitochondria Isolated from Conidia of Neurospora crassa

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