Heat-induced Macroconidia Germination in Microsporum gypseum

Leighton, Terrance; Stock, J. J.

doi:10.1128/aem.17.3.473-475.1969

Cited by 15 publications

(17 citation statements)

References 5 publications

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“…2). Isolation of mature macroconidia and germination procedures have been described previously (10). Photographs of lactophenol cotton blue-stained specimens were taken with a Zeiss microscope (green filter).…”

Section: Methodsmentioning

confidence: 99%

“…The number of days required for mature spore formation is shown as circled numerals associated with the growth curves of strain R87 (0), or strain R87P1 (0). Incubation of mature strain R87 macroconidia on the shift-down medium is represented by 0 days submerged culture growth, and (e) represents no spores formed after 10 days incubation after shift-down, 27 C. physiological saline (37 C), but did form discrete germ tubes in nutrient media (37 C). The conidia were fertile and formed typical pleomorphic strain colonies which sporulated very sparsely in 12 to 14 days without additional aeration as did the parent pleomorphic colony.…”

Section: _2mentioning

confidence: 99%

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Initiation of Dermatophyte Pleomorphic Strain Sporulation by Increased Aeration

Page¹,

Stock²

1972

Applied Microbiology

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Section: Methodsmentioning

confidence: 99%

Section: _2mentioning

confidence: 99%

Initiation of Dermatophyte Pleomorphic Strain Sporulation by Increased Aeration

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Stock²

1972

Applied Microbiology

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“…The strain of organism, sporulation, and germination conditions were described previously (4,5). All experiments were carried out with physiological saline, 0.85% NaCl, w/v (pH 6.5), as the germination medium.…”

Section: Methodsmentioning

confidence: 99%

“…All experiments were carried out with physiological saline, 0.85% NaCl, w/v (pH 6.5), as the germination medium. Spore germinations were determined microscopically as previously reported (4). Mycelial and spore nuclei were stained by the Giemsa method of Hejtmanek and Hejtmankova-Uhrovd (3) or the aceto-orcein method of El-Ani (1).…”

Section: Methodsmentioning

confidence: 99%

Relationship of Nuclear Segregation and Macroconidial Germination in Microsporum gypseum

Leighton

Stock

1970

J Bacteriol

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Microsporum gypseum macroconidia germinated at 37 C possessed from one to eight nuclei per germinated spore compartment. The distribution of nuclei per spore compartment was the result of a random packaging of nuclei from the available nuclear population. Partial inhibition of germination by incubation at 25 C or at 37 C in the presence of 10-4 M phenyl methyl sulfonyl-fluoride resulted in an enrichment of germinated spores containing high numbers of nuclei per compartment. The selection for higher nuclear numbers was statistically significant. Compartments possessing high numbers of nuclei appeared to be precommitted to spore germination since they were not sensitive to germination inhibition. The effect of incubation temperature variation on spore germination is discussed with respect to the organism's natural environment.Asexual sporulation in fungi results in the production of uninucleate or multinucleate (or both) spore types (1, 2, 6, 7). The multinucleate spores are characterized by a varying number of nuclei per spore or septated spore unit. Recently, Heisenberg and Cerdac-Olmedo (2) demonstrated that

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“…MATERIALS AND METHODS Organisms. The origin, growth, and sporulation characteristics of M. gypseum strain R87 have been described previously (10). A pleomorphic strain (R87P1), derived as a spontaneous mutant of the wild-type strain R87 and also used in this study, was free from microconidia and macroconidia during the conditions of the normal wild-type sporulation cycle (19).…”

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Changes inMicrosporum gypseumMycelial Wall and Spore Coat Glycoproteins During Sporulation and Spore Germination

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Stock

1974

J Bacteriol

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Ethylenediamine-soluble glycoproteins were extracted from isolated Microsporum gypseum hyphal walls during sporulation and from spore coats before and after germination. This study was carried out to identify a sporulationspecific cell wall protein that possibly served as a substrate for the alkaline protease which initiated the macroconidial germination of this fungus. Analyses revealed that water-insoluble glycoprotein accounted for 10% of the ungerminated spore coat but only for 4 to 5% of the mycelial wall dry weight. This fraction was modified in its amino acid composition during sporulation, and it decreased in protein content during spore germination. Water-soluble glycoprotein, which accounted for approximately 3 to 3.5% of either the spore coat or mycelial wall dry weight, was of similar amino acid composition from both sources and did not decrease in protein content upon spore germination. The water-insoluble glycoprotein was found to be rich in leucine, aspartic acid, glycine, glutamic acid, and phenylalanine residues. The water-soluble glycoprotein was rich in proline, threonine, glycine, serine, glutamnic acid, and alanine.Spores were purified and germinated as described previously (10).

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Heat-induced Macroconidia Germination in Microsporum gypseum

Abstract: A method for obtaining purified ungerminated macroconidia is described, and a technique for obtaining 85 to 90% germination of macroconidia under normal nutritional conditions is presented.

Cited by 15 publications

References 5 publications

Initiation of Dermatophyte Pleomorphic Strain Sporulation by Increased Aeration

Initiation of Dermatophyte Pleomorphic Strain Sporulation by Increased Aeration

Relationship of Nuclear Segregation and Macroconidial Germination in Microsporum gypseum

Changes inMicrosporum gypseumMycelial Wall and Spore Coat Glycoproteins During Sporulation and Spore Germination

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