Heat‐killed Pseudoalteromonas piscicida 2515 decreased bacterial dose and improved immune resistance against Vibrio anguillarum in juvenile olive flounder ( Paralichthys olivaceus )

Abstract

Pathogenic Vibrio spp. are Gram-negative bacteria and can infect many fish and shellfish species including olive flounder (Paralichthys olivaceus) (Lee et al., 2021). Such bacteria cause vibriosis via gastric invasion in marine species (Chen et al., 2003), exhibiting typical hemorrhagic septicemia, lethargy and dark skin lesions in infecting olive flounder. Vibrio spp. have caused mass mortalities and extensive economic losses of olive flounder aquaculture industry in

The pathogen Vibrio alginolyticus H1 and its antagonist Pseudoalteromonas piscicida H2 associated with the health status of cuttlefish Sepia pharaonis

“…Yet, in our present study, P. piscicida H2 showed no virulence during the 7 d infection. Previous reports have showed that probiotics P. piscicida might act as bio-agents to control Vibrio infection in pearl gentian groupers and shrimp Penaeus vanname [ 57 , 58 , 39 ]. As shown in our results, pathogen V. alginolyticus H1 was successfully isolated and characterized in the case of skin ulcers.…”

Whole-genome sequencing of Pseudoalteromonas piscicida 2515 revealed its antibacterial potency against Vibrio anguillarum: a preliminary invitro study

“…Antimicrobial activity is regarded as an essential approach by which probiotics ght against pathogens in the gut. Previous studies have shown that P. piscicida 2515 has broad-spectrum antibacterial activity, and dietary supplementation with P. piscicida 2515 has a positive effect on shrimp and olive ounder intestinal microbiota (Wang et al, , 2022. The availability of whole-genome sequence information and the variety of genome mining tools make it possible to predict the antimicrobial compounds of this bacterium and their underlying mechanism.…”

“…In our previous study, P. piscicida 2515 showed haemolytic activity on sh blood cells (Zhang et al, 2021). Moreover, heat-killed P. piscicida 2515 could enhance nonspeci c immunity and resistance against V. anguillarum in olive ounder aquaculture (Wang et al, 2022). In addition, virulence genes, including enterotoxin FM (entFM), cereulide (ces), and cytotoxin K, were not detected in the P. piscicida 2515 genome.…”

“…In addition, virulence genes, including enterotoxin FM (entFM), cereulide (ces), and cytotoxin K, were not detected in the P. piscicida 2515 genome. After thermal exposure, the haemolytic effect of P. piscicida 2515 was eliminated (Zhang et al, 2021), and the administration of this bacterium has potent and safe application prospects in sh and shrimp aquaculture (Wang et al, , 2022. Further comprehensive investigations, including food biosecurity and human health aspects, must be considered.…”

“…In this study, Pseudoalteromonas. piscicida strain 2515 was introduced as a potential probiotic for shrimp and olive ounder aquaculture since it shows a broad anti-Vibrio effect (Wang et al, , 2022. However, it is important to investigate its probiotic characteristics prior to aquaculture application (Farzanfar, 2006;Wanna et al, 2021).…”