Heat Pretreatment Alleviates UV‐B Toxicity in the CyanobacteriumAnabaena doliolum: A Proteomic Analysis of Cross Tolerance

Abstract

This study offers proteomic elucidation of heat pretreatment-induced alleviation of UV-B toxicity in Anabaena doliolum. Heat-pretreated cells exposed to UV-B showed improved activity of PSI, PSII, whole chain, (14)C fixation, ATP and NADPH contents compared to UV-B alone. Proteomic analysis using two-dimensional gel electrophoresis (2-DE), MALDI-TOF MS/MS and reverse transcription polymerase chain reaction (RT-PCR) of UV-B and heat pretreatment followed by UV-B-treated cells exhibited significant and reproducible alterations in nine proteins homologous to phycocyanin-alpha-chain (PC-alpha-chain), phycoerythrocyanin-alpha-chain (PEC-alpha-chain), hypothetical protein alr0882, phycobilisome core component (PBS-CC), iron superoxide dismutase (Fe-SOD), fructose-1,6-bisphosphate aldolase (FBA), nucleoside diphosphate kinase (NDPK), phosphoribulokinase (PRK) and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) large chain. Except the PEC-alpha-chain, hypothetical protein alr0882 and PBS-CC, all other proteins showed upregulation at low doses of UV-B (U2) and significant downregulation at higher doses of UV-B (U5). The disruption of redox status, signaling, pentose phosphate pathway and Calvin cycle appears to be due to the downregulation of Fe-SOD, NDPK, FBA, PRK and RuBisCo thereby leading to the death of Anabaena. In contrast to this, the upregulation of all the above proteins in heat-pretreated cells, harboring different heat shock proteins (HSPs) like 60, 26 and 16.6, followed by UV-B treatment than only the UV-B-treated ones suggests a protective role of HSPs in mitigating UV-B toxicity.

Proteomics combines morphological, physiological and biochemical attributes to unravel the survival strategy of Anabaena sp. PCC7120 under arsenic stress

“…6). This is supported by our earlier data on the downregulation of AhpC and upregulation of Prx in A. doliolum under heat stress [10]. Prx has multiple roles like (i) reduction of H 2 O 2 and organic hydroperoxides [95], (ii) acting as a molecular chaperone similar to HSPs [96], and (iii) modulation of cell signaling.…”

“…Since proteins are directly involved in stress responses, proteomic studies can unravel the possible relationships between protein abundance and stress acclimation. Over the past years, the proteomic approach has been applied to analyze the proteins involved in stress responses in cyanobacteria, such as Cu [6], salinity [7,8], heat and UV-B [9][10][11], butachlor [12], and Fe starvation [13]. However, As-induced proteomic changes in cyanobacteria have remained unexplored as yet.…”

Biochemical traits and proteomic changes in postharvest flowers of medicinal chrysanthemum exposed to enhanced UV-B radiation

“…In our study, nucleoside diphosphate kinase protein was up-regulated by enhanced UV-B radiation. These results are also supported by Mishra et al [55]. Up-regulation of nucleoside diphosphate kinase is mainly responsible for maintenance of cellular redox homeostasis.…”

Proteomic Analysis of Cucumber Defense Responses Induced by Propamocarb

“…At the same time, proteomics has emerged as a tool to investigate the cellular responses to toxicants and to uncover new acclimation mechanisms (Yan et al 2005). Proteomics has been applied to study under abiotic treatment included in copper-induced changes (Bhargava et al 2008), the impact of salt (Srivastava et al 2008), circadian variation (Feng et al 2012), and heat and UV-B stresses (Mishra et al 2009).…”