Heat resistance and heat-shock protein synthesis in a reptilian cell line, IgH-2, from Iguana iguana

Bols, Niels C.; Steels, G.B.; Mosser, Dick D.; Heikkila, John J.

doi:10.1016/0306-4565(90)90034-f

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…3). A similar phenomenon was reported by Bols et al (1990) using reptilian IgH-2 cells from Iguana iguana. These cells are routinely grown at 36°C, but at temperatures above 42°C cytoplasmic vacuoles appeared, cell bodies rounded with extended cell processes, shriveling became apparent, and cells eventually detached from the growth surface.…”

Section: Mentssupporting

confidence: 86%

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In Vitro Thermal Effects on Embryonic Cells of Endangered Hawksbill TurtleEretmochelys imbricata

et al. 2013

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The hawksbill turtle is an ectotherm, whose sex is determined by temperature during embryonic development. This study aimed to determine whether embryonic hawksbill turtle cells respond differently to temperature than mammalian cells. Embryonic hawksbill turtle cells were established in culture, and thermal effects on these cells were investigated in vitro. Cells were maintained in Dulbecco's Modified Eagle Medium supplemented with non-essential amino acids, vitamin solution, sodium pyruvate, and 10% fetal bovine serum at 33°C and cell proliferation occurred at 25-33°C. When cells were incubated at 37°C (the temperature of mammalian cell culture) for 24 h, cell growth was completely inhibited. This growth inhibition was evidently recovered by changing the incubation temperature back to 33°C. Expression of heat shock protein was found to increase with elevating culture temperature from 25 to 33°C.

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Section: Mentssupporting

confidence: 86%

“…One unique report demonstrated that bacterial infection causes a tissuespecific increase in the expression of hsp73, hsp72 and hsp90 in C. picta belli . In a reptilian cell line from Iguana iguana, synthesis of hsps comprising polypeptides of 87,76,70,29,28,22, and 21 kDa were identified (Bols et al, 1990).…”

Section: Mentsmentioning

confidence: 99%

In Vitro Thermal Effects on Embryonic Cells of Endangered Hawksbill TurtleEretmochelys imbricata

et al. 2013

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“…Methods Tunicamycin treatment has been used to identify a specific set of polypeptides : the glucose-regulated proteins (GRPs). Exposure of mammalian [32], avian [24], reptilian [4] and amphibian [35] cell cultures to tunicamycin consistently enhances the synthesis of two polypeptides, whose sizes fall into the 73-80 kDa and 94-100 kDa ranges and are referred to respectively as GRP78 and GRP94 [I 5, 161. Tunicamycin, an antibiotic, inhibits protein glycosylation [30].…”

Section: Introductionmentioning

confidence: 99%