Heat-shock induced somatic embryogenesis in callus cultures of gladiolus in the presence of high sucrose

Kumar, Anil; Palni, L. M. S.; Sood, Anil K.; Sharma, Madhu; Palni, Uma T.; Gupta, Akshey K.

doi:10.1080/14620316.2002.11511460

Cited by 20 publications

(24 citation statements)

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“…In the present study, increased shoot growth was observed following heat shock on BM at sucrose concentrations 58 -116 mM, whereas, sucrose concentrations higher than 174 mM and heat shock inhibited shoot growth (Table 3). Interaction of different sucrose concentrations and heat shock on regulation of morphogenesis was also reported in gladiolus (Kumar et al 1999(Kumar et al , 2002. 4.9 ± 0.2fg 3.6 ± 0.1cd 2.5 4.7 ± 0.1de 3.0 ± 0.1ef 5.0 6.2 ± 0.1c 2.3 ± 0.1g 12.5 6.8 ± 0.2bc 1.9 ± 0.1gh…”

Section: Resultsmentioning

confidence: 74%

Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

Kumar¹,

Aggarwal²,

Gupta³

et al. 2010

Biologia plant.

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The effect of plant growth regulators (PGRs), gelling agents, sucrose and heat shock on shoot multiplication, shoot growth, rooting and subsequent survival of Chlorophytum borivilianum Sant. et Fernand was evaluated. Benzyladenine (BA) was found to be better cytokinin over kinetin (KIN) for shoot multiplication. Sucrose concentrations from 116 -290 mM in the basal medium (BM) promoted shoot multiplication. Heat shock (50 °C, 1 h) also promoted shoot multiplication at these sucrose concentrations on both BM medium and BM supplemented with 5.0 µM BA. Beneficial effect of sucrose was also observed on rooting of shoots on BM as well as BM supplemented with 5.0 μM indole-3-butyric acid (IBA). Phytagel as a gelling agent was found to be more effective for shoot proliferation and growth compared to agar. Amongst various soil mixtures tested, higher survival of plants was observed in soil containing Vermicompost. It was interesting to note that a maximum plant survival (> 95 %) was observed when plants were directly transferred to net-house (irradiance reduced to 50 % with green net, without humidity and temperature control) than poly-house (with humidity and temperature control). Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analysis of regenerated plants showed genetic similarity to mother plant.

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Section: Resultsmentioning

confidence: 74%

Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

Kumar¹,

Aggarwal²,

Gupta³

et al. 2010

Biologia plant.

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“…Secondary embryos were initiated from incompletely formed primary embryos that were still developing in the medium. A heat shock (50°C for 1 h) significantly increased somatic embryo formation on callus grown at high sucrose levels (12%), but no embryogenesis occurred at lower levels (2%, Kumar et al 2002). Addition of putrescine to media with high sucrose could also increase somatic embryogenesis, but not as much as the heat shock treatment.…”

Section: Somatic Embryogenesismentioning

confidence: 85%

Micropropagation of iridaceae—a review

Ascough

Erwin

Staden

2009

Plant Cell Tiss Organ Cult

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The Iridaceae contains many hundreds of attractive species that have been overlooked for horticultural development. The incredible variation in flower and leaf shape, size and colour suggest this little-tapped resource could offer great opportunities for developing new commercial ornamental products. Micropropagation has increasingly become a valuable tool assisting breeders to release new species and cultivars into the market more rapidly. Here we review the progress made in Iridaceae micropropagation genus by genus, and highlight the potential for future expansion in this field.

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“…A large number of plants/propagules can be produced in a relatively shorter time from disease-free cultures. In view of this, a large number of studies have concentrated on in vitro propagation of gladiolus using micropropagation techniques (Dantu and Bhojwani 1992;Dutta-Gupta and Datta 2004;Ginzburg and Ziv 1973;Hussey 1977;Kim et al 1988;Kumar et al 1999Kumar et al , 2002Prasad and Dutta-Gupta 2006;Rao et al 1991;Remotti and Loffler 1995;Sen and Sen 1995;Ziv et al 1970) and also through cormlet formation Bhojwani 1987, 1995;De Bruyn and Ferreira 1992;Roy et al 2006;Steinitz and Lilien-Kipnis 1989;Steinitz et al 1991;Ziv 1989). These reports appear to be inconsistent and show variations in response within cultivars.…”

Section: Introductionmentioning

confidence: 87%

Factors affecting in vitro formation of cormlets in Gladiolus hybridus Hort. and their field performance

2010

Self Cite

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The effect of number of important factors on in vitro cormlet formation has been investigated in Gladiolus hybridus Hort. Sucrose concentration of 232 mM was found to be best for producing higher number of cormlets per flask, whereas, the average mass of a cormlet increased with increase in sucrose concentration. Amongst three cultivars (cvs), maximum number of cormlets produced per flask was recorded in cv 'Her Majesty', but the average cormlet mass was higher in case of cv 'Bright Eye'. Although the number of cormlets produced was found to be marginally higher at 30°C when compared with 20°C, the average cormlet mass was higher at the lower temperature. Both the number of cormlets formed per culture flask as well as the average fresh mass of a cormlet increased with increase in the size (volume) of the culture flask used. The known inhibitors of gibberellin biosynthesis used in this study suppressed cormlet formation, and the maximum inhibition was recorded in case of maleic acid hydrazide. Polyamines were found to be beneficial for cormlet formation, and amongst the polyamines used, incorporation of spermidine in the culture medium resulted in maximum number of cormlet formation per culture flask. Field trials indicated that the performance of such in vitro produced cormlets was comparable to that of conventionally produced cormels of the same weight range. The plants raised from in vitro produced cormlets were found to be morphologically similar to the mother plant.

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Heat-shock induced somatic embryogenesis in callus cultures of gladiolus in the presence of high sucrose

Cited by 20 publications

References 0 publications

Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

Micropropagation of iridaceae—a review

Factors affecting in vitro formation of cormlets in Gladiolus hybridus Hort. and their field performance

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