Heat shock protein 60 (GroEL) fromPorphyromonas gingivalis: Molecular cloning and sequence analysis of its gene and purification of the recombinant protein

Establishment of B-1 cell-derived polyreactive monoclonal antibodies and expression of costimulators by B-cell to antigenic stimulation

“…LPS (Sigma) from Escherichia coli 0111:B4, Mahsp [Recombinant Mouse Hsp60 Protein (Stressgen, Victoria, BC, Canada)], DNA from calf thymus (Sigma) and PC-KLH (Biosearch Technologies, Inc., Novata, CA, USA) were used in this experiment. Recombinant Pghsp, was purified from Escherichia coli transformed with P. gingivalis GroEL 21) . Peroxidase-labeled affinity purified antibodies to mouse IgM (μ) produced in goats were purchased from KPL (Gaithersburg MD, USA).…”

Development of monoclonal antibody against Porphyromonas gingivalis heat shock protein

“…HSP 유전자는 P. gingivalis 17) , A. actinomycetemcomitans 18) , B. forsythus 19) , T. denticola 20) , F. nucleatum 21) , C. rectus 22) 와 P. …”