Heat shock protein 60: Identification of specific epitopes for binding to primary macrophages

Habich, Christiane; Kempe, Karina; Gómez, Francisco; Lillicrap, Mark; Gaston, Hill; Zee, Ruurd van der; Kolb, Hubert; Burkart, Volker

doi:10.1016/j.febslet.2005.11.060

Cited by 48 publications

(30 citation statements)

References 34 publications

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“…In case there was binding of Hsp60 via surface receptors, the expected consequence would be activation of CD68-positive cells followed by inflammatory response, as shown in the in vitro models. [49][50][51] All together, these results support the working hypothesis that Hsp60 can play a decisive role in the pathogenesis of UC, and make Hsp60 a promising candidate for the treatment of UC with antichaperonin agents.…”

Section: Discussionsupporting

confidence: 71%

Changes in Immunohistochemical Levels and Subcellular Localization After Therapy and Correlation and Colocalization With CD68 Suggest a Pathogenetic Role of Hsp60 in Ulcerative Colitis

Tomasello

Rodolico²,

Zerilli³

et al. 2011

Applied Immunohistochemistry &Amp; Molecular Morphology

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Abstract:In an earlier work, the role of heat shock protein (Hsp60) in the pathogenesis of ulcerative colitis (UC) was suggested by its significant increase in the pathological mucosa parallel with an increase in inflammatory cells. More data in this direction are reported in this work. We analyzed by immunohistochemistry biopsies of colon tissue from 2 groups of patients with UC and treated with either 5-aminosalicylic acid (5-ASA) alone or in combination with a probiotic. We looked for inflammatory markers and Hsp60. Both the treatments were effective in reducing symptoms but the group treated with both 5-ASA and probiotics showed better clinical results. Amelioration of symptoms was associated with reduction of both inflammation and Hsp60, a reduction that was most marked in the group treated with 5-ASA and probiotics. The levels of Hsp60 positively correlated with those of CD68-positive cells, and double immunofluorescence showed a high index of colocalization of the chaperonin and CD68 in lamina propria. Immunoelectron microscopy showed that Hsp60Fclassically a mitochondrial proteinFwas abundantly also present in cytosol in biopsies taken at the time of diagnosis, but not after the treatment. Our data suggest that Hsp60 is an active player in pathogenesis of UC and it can be hypothesized that the chaperonin is responsible, at least in part, for initiation and maintenance of disease.

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Section: Discussionsupporting

confidence: 71%

Changes in Immunohistochemical Levels and Subcellular Localization After Therapy and Correlation and Colocalization With CD68 Suggest a Pathogenetic Role of Hsp60 in Ulcerative Colitis

Tomasello

Rodolico²,

Zerilli³

et al. 2011

Applied Immunohistochemistry &Amp; Molecular Morphology

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“…This can be proven by following facts: their availability in both eukaryotic and prokaryotic organisms; presence of highly conserved sequences in the structure of HSP, which are also immunodominant; high immunologic activity of HSP. The HSPdependent system (network) of inflammation and autoimmu nity stimulation and regulation includes, at any rate, following elements: HSP as polyreactive antigens; natural auto antibodies and anti-idiotypic antibodies; proteins of macro organism as such released from the cells due to various reasons; foreign proteins (including HSP of microorganisms); antigen-presenting cells that can be activated by HSP; effector and regulatory T-lymphocytes interacting with HSP [12][13][14][15].…”

Section: Resultsmentioning

confidence: 99%

“…In view of several particulars of formation of B-cell response to heat shock proteins [12][13][14][15], as well as reports on potential effects of antiidiotypic serum antibodies on the nature of immunochemical reactions (e.g. socalled prozone effect in tests based on agglutination or precipitation reaction) [16][17][18]; additionally, development of indirect ELISA for identification of anti-HSP-60 IgG-antibodies using biotinylated tyramine reagent was performed.…”

Section: Resultsmentioning

confidence: 99%

Characteristics of enzyme-linked immunosorbent assay for detection of IgG antibodies specific to Сhlamydia trachomatis heat shock protein (HSP-60)

Galkin¹,

Besarab²,

Lutsenko³

2017

Ukr.Biochem.J.

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e x p e r i m e n t a l w o r k s e x p e r i m e n t a l w o r k s UDC 573.6.083.3+573.6.086.8 CharaCteristiCs of enzyme-linked immunosorbent assay for deteCtion of igg antibodies speCifiC to Сhlamydia trachomatis heat shoCk protein (hsp-60) O. Yu. GalkIN, a. B. BeSaraB, T. N. lUTSeNkO National registered). In 4 out of 15 intralaboratory panel serum samples initially identified as nega tive, anti-HSP-60 IgG-antibodies test result in reference ELISA sets upon dilution changed from negative to positive. The nature of titration curves of false negative sera and commercial monoclonal antibodies А57-В9 against C. trachomatis HSP-60 after incubation for 24 h was indicative of the presence of anti-idiotypic antibodies in these samples. Upon sera dilution, idiotypic-anti-idiotypic complexes dissociated, which caused the change of test result. High informative value of the developed ELISA set for identification of IgG antibodies against C. trachomatis HSP-60 has been proven. Anti-idiotypic antibodies possessing C. trachomatis anti-HSP-60 activity and being one of the causes of false negative results of the relevant elISa-based tests have been identified in blood sera of individuals infected with chlamydial genitourinary infection agents. K e y w o r d s: enzyme-linked immunosorbent assay, Chlamydia trachomatis, heat shock protein, anti-idiotypic antibodies, sensitivity, specificity.C hlamydial genitourinary infection (GUI) is one of the most common sexually transmitted infections. According to World Health Organization, Chlamydia trachomatis infects about 90 million people each year by sexual transmission. In Ukraine, the rate of chlamydial GUI is 80 cases per 100,000 population. Nearly 16% of pregnant women are infected with C. trachomatis. About 50-60% of tubular infertility cases are caused by chlamydial infection. A quarter of all ophthalmic and respiratory diseases in newborn and younger children are associated with chlamydial infection [1,2].Effective diagnosis is one of important components of chlamydial GUI control, which can be realized through both direct (detection of antigens, nucleic acids, pathogenic agent microscopic examination and cultivation) and indirect (specific antibo dies detection) techniques. One of the commonly used methods in diagnostics is enzyme-linked immunosorbent assay (ELISA) that allows differential diagnosis -determination of disease stage and course, which is especially important in chronic conditions. For that purpose blood serum (plasma) and human biological fluids are tested for the presence of IgM, IgA and IgG classes of specific antibodies to pathogen's antigens [2,3].Prolonged persistency of C. trachomatis increases the expression of heat shock protein with molecular mass 60 kDa (HSP-60), which is highly

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“…The vaccination of mice with Hsp60 confers protection from Histoplasma through both cellular (CD4 ϩ T cells [7]) and humoral immune mechanisms (11). In addition, Hsp60 can function as an adhesin for yeast binding to complement receptors on host macrophages (12,19). Indicating the importance of ameliorating heat stresses, virulence differences among Histoplasma strains are correlated with in vitro thermosensitivity and differences in heat shock response (5).…”

mentioning

confidence: 99%

Discovery of a Role for Hsp82 in Histoplasma Virulence through a Quantitative Screen for Macrophage Lethality

2011

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The application of forward genetics can reveal new factors required for the virulence of intracellular pathogens. To facilitate such virulence screens, we developed macrophage cell lines with which the number of intact host cells following infection with intracellular pathogens can be rapidly and easily ascertained through the expression of a constitutive lacZ transgene. Using known virulence mutants of Francisella novicida and Histoplasma capsulatum, we confirmed the applicability of these host cells for the quantitative assessment of bacterial and fungal virulence, respectively. To identify new genes required for Histoplasma virulence, we employed these transgenic macrophage cells to screen a collection of individual transfer DNA (T-DNA) insertion mutants. Among the mutants showing decreased virulence in macrophages, we identified an insertion in the locus encoding the Histoplasma Hsp82 homolog. The lesion caused by the T-DNA insertion localizes to the promoter region, resulting in significantly decreased HSP82 expression. Reduced HSP82 expression markedly attenuates the virulence of Histoplasma yeast in vivo. While the HSP82 hypomorph grows normally in vitro at 37°C and under acid and salinity stresses, its ability to recover from high-temperature stress is impaired. These results provide genetic proof of the role of stress chaperones in the virulence of a thermally dimorphic fungal pathogen.

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Heat shock protein 60: Identification of specific epitopes for binding to primary macrophages

Cited by 48 publications

References 34 publications

Changes in Immunohistochemical Levels and Subcellular Localization After Therapy and Correlation and Colocalization With CD68 Suggest a Pathogenetic Role of Hsp60 in Ulcerative Colitis

Changes in Immunohistochemical Levels and Subcellular Localization After Therapy and Correlation and Colocalization With CD68 Suggest a Pathogenetic Role of Hsp60 in Ulcerative Colitis

Characteristics of enzyme-linked immunosorbent assay for detection of IgG antibodies specific to Сhlamydia trachomatis heat shock protein (HSP-60)

Discovery of a Role for Hsp82 in Histoplasma Virulence through a Quantitative Screen for Macrophage Lethality

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