Heat-shock protein 70-dependent dendritic cell activation by 5-aminolevulinic acid-mediated photodynamic treatment of human glioblastoma spheroids in vitro

Etminan, Nima; Peters, Corinna; Lakbir, Dalila; Bünemann, Erich; Börger, Verena; Sabel, Michael; Hänggi, Daniel; Steiger, H J; Stummer, Walter; Sorg, Rüdiger V.

doi:10.1038/bjc.2011.327

Cited by 70 publications

(51 citation statements)

References 41 publications

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“…Thus, since several CRT translocation pathways have been described [10], [42], it would be interesting to investigate whether the different PSs induce the externalization of CRT following different molecular pathways. HSP70 externalization induced by RBAc-PDT is in line with data in literature reporting results obtained with different PSs, such as Hyp- [10], Photofrin- [21], [25], [26], Foscan [27], and 5-ALA [28]. In RBAc-PDT externalization of HSP70 is an early event (1 h) after irradiation and it is independent on the cell death type (apoptosis or autophagy); however, the amount of translocated HSP70 is greater in apoptotic cells than autophagic ones suggesting cell death type dependency.…”

Section: Discussionsupporting

confidence: 91%

Rose Bengal Acetate PhotoDynamic Therapy (RBAc-PDT) Induces Exposure and Release of Damage-Associated Molecular Patterns (DAMPs) in Human HeLa Cells

et al. 2014

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The new concept of Immunogenic Cell Death (ICD), associated with Damage Associated Molecular Patterns (DAMPs) exposure and/or release, is recently becoming very appealing in cancer treatment. In this context, PhotoDynamic Therapy (PDT) can give rise to ICD and to immune response upon dead cells removal. The list of PhotoSensitizers (PSs) able to induce ICD is still short and includes Photofrin, Hypericin, Foscan and 5-ALA. The goal of the present work was to investigate if Rose Bengal Acetate (RBAc), a powerful PS able to trigger apoptosis and autophagy, enables photosensitized HeLa cells to expose and/or release pivotal DAMPs, i.e. ATP, HSP70, HSP90, HMGB1, and calreticulin (CRT), that characterize ICD. We found that apoptotic HeLa cells after RBAc-PDT exposed and released, early after the treatment, high amount of ATP, HSP70, HSP90 and CRT; the latter was distributed on the cell surface as uneven patches and co-exposed with ERp57. Conversely, autophagic HeLa cells after RBAc-PDT exposed and released HSP70, HSP90 but not CRT and ATP. Exposure and release of HSP70 and HSP90 were always higher on apoptotic than on autophagic cells. HMGB1 was released concomitantly to secondary necrosis (24 h after RBAc-PDT). Phagocytosis assay suggests that CRT is involved in removal of RBAc-PDT generated apoptotic HeLa cells. Altogether, our data suggest that RBAc has all the prerequisites (i.e. exposure and/or release of ATP, CRT, HSP70 and HSP90), that must be verified in future vaccination experiments, to be considered a good PS candidate to ignite ICD. We also showed tha CRT is involved in the clearance of RBAc photokilled HeLa cells. Interestingly, RBAc-PDT is the first cancer PDT protocol able to induce the translocation of HSP90 and plasma membrane co-exposure of CRT with ERp57.

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Section: Discussionsupporting

confidence: 91%

Rose Bengal Acetate PhotoDynamic Therapy (RBAc-PDT) Induces Exposure and Release of Damage-Associated Molecular Patterns (DAMPs) in Human HeLa Cells

et al. 2014

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“…The best characterized DAMPs involved in PDT-triggered cell death able to confer immunogenicity are HSPs proteins and especially HSP70, as described for squamous cells carcinoma SCCVII [203], murine mammary tumor cells C127 [206] during Photofrin-PDT, EMT6 cells during meso-tetrahydroxyphenyl chlorin (mTHPS, Foscan)-PDT [207] and glioblastoma cell lines U87 and U251 during 5-ALA-PDT [208]. …”

Section: The Response Of Immune System To Photodynamic Treatmentmentioning

confidence: 99%

Immunogenic Cell Death: Can It Be Exploited in PhotoDynamic Therapy for Cancer?

Panzarini

Inguscio

Dini

2013

BioMed Research International

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Immunogenic Cell Death (ICD) could represent the keystone in cancer management since tumor cell death induction is crucial as well as the control of cancer cells revival after neoplastic treatment. In this context, the immune system plays a fundamental role. The concept of Damage-Associated Molecular Patterns (DAMPs) has been proposed to explain the immunogenic potential of stressed or dying/dead cells. ICD relies on DAMPs released by or exposed on dying cells. Once released, DAMPs are sensed by immune cells, in particular Dendritic Cells (DCs), acting as activators of Antigen-Presenting Cells (APCs), that in turn stimulate both innate and adaptive immunity. On the other hand, by exposing DAMPs, dying cancer cells change their surface composition, recently indicated as vital for the stimulation of the host immune system and the control of residual ill cells. It is well established that PhotoDynamic Therapy (PDT) for cancer treatment ignites the immune system to elicit a specific antitumor immunity, probably linked to its ability in inducing exposure/release of certain DAMPs, as recently suggested. In the present paper, we discuss the DAMPs associated with PDT and their role in the crossroad between cancer cell death and immunogenicity in PDT.

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“…3D co-cultures of normal brain cells and glioblastoma-derived cells have been set up by Biggs and coworkers [219]. Normal brain cells were isolated from cortices of Wistar rats and allowed to reaggregate and to mature for 20 days, by placing them on a semi-porous membrane at an air-liquid interface, and subsequently generating characteristic brain HiSpots [220,221].…”

Section: D Co-cultures Of Tumoral Cns Cells and Normal Brain Cellsmentioning

confidence: 99%

“…The authors could observe their spread throughout the Hi-Spots, forming new cell aggregates, and able to last for many days (25 d) in culture. This platform was then tested for evaluating anticancer compounds, thus representing a simple system to verify drug effectiveness on brain tumors in an orthotopic environment [219].…”

Section: D Co-cultures Of Tumoral Cns Cells and Normal Brain Cellsmentioning

confidence: 99%

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

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A large body of evidence indicates that three dimensional (3D) cancer models are superior to two-dimensional (2D) ones in better representing the in vivo phenomena. Indeed, 3D models allow recapitulating in vitro the in vivo features observed in solid tumors (e.g. cell polarity, cell-cell/cell-matrix interactions, biochemical/metabolic gradients, anchorage-independent growth and hypoxia). Moreover, it is well established that the microenvironment plays a fundamental role in regulating tumor development and behavior, including drug resistance. Thus, innovative models able to mimic this complexity represent attractive tools in cancer research. In this review article, we provide a comprehensive review of the application of 3D culture systems in pediatrics' cancer research. In particular, 3D in vitro/ex vivo models of the most common pediatric tumors, such as leukemias, lymphomas and malignancies of the nervous system, will be considered.

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Heat-shock protein 70-dependent dendritic cell activation by 5-aminolevulinic acid-mediated photodynamic treatment of human glioblastoma spheroids in vitro

Cited by 70 publications

References 41 publications

Rose Bengal Acetate PhotoDynamic Therapy (RBAc-PDT) Induces Exposure and Release of Damage-Associated Molecular Patterns (DAMPs) in Human HeLa Cells

Rose Bengal Acetate PhotoDynamic Therapy (RBAc-PDT) Induces Exposure and Release of Damage-Associated Molecular Patterns (DAMPs) in Human HeLa Cells

Immunogenic Cell Death: Can It Be Exploited in PhotoDynamic Therapy for Cancer?

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

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