Heat shock protein 70 expression in different tissues of Cirrhinus mrigala (Ham.) following heat stress

Abstract

Members of heat shock protein 70 (HSP70) are highly conserved proteins of about 70 kDa and play important roles in protein folding. Levels of these proteins increase when cells are under stress. Environmental temperature influences both the basal and induced levels of HSPs. However, studies on HSPs in fishes from a tropical country such as India are lacking. In the present study, Indian major carp (IMC) Cirrhinus mrigala (Ham.) acclimatized at 25±2°C had high levels of HSP70, viz., 1.2–1.3 ng μg−1 total protein in kidney and gill and 4.2–5.3 ng μg−1 total protein in liver and brain tissues, indicating the presence of biochemically significant levels of stress. However, maintenance of acclimatized fish at 17°C for up to 48 h did not lead to a significant decrease in stress protein levels. A heat shock at 37°C for up to 48 h resulted in only two to threefold increase in HSP70 levels in these organs. Although the increase in HSP70 levels was apparent from the first hour of heat stress in all these tissues, the increase was significant from the second hour in the brain, the sixth hour in liver and kidney and the 20th hour in the gills.

Feeding rates affect heat shock protein levels in liver of larval white sturgeon(Acipenser transmontanus)

“…Dietz (1994) observed that the Hsp70 response was absent in brain of Gillichthis mirabilis but present in liver. In contrast, Dias et al (2005) found a strong induction of Hsp70 in liver but not in kidney and gill after heat stress. No information is available on comparison of Hsp level among different tissues in white sturgeon.…”

Involvement of p38MAPKin the Induction ofHsp70 During Acute Thermal Stress in Red Blood Cells of the Gilthead Sea Bream,Sparus aurata

“…The expression of Hsp70 in the RBCs of S. aurata under acute thermal stress is consistent with the HSR of RBCs from other fish species (Currie and Tufts, ; Currie et al, ; Lund et al, ; Heredia‐Middleton et al, ). It has been shown that the induction of Hsp70 is more evident compared with other Hsps after thermal stress (Das et al, ). The reduction in the levels of Hsp70 after treatment of RBCs from S. aurata with the inhibitor of p38 MAPK, SB203580, indicates that phosphorylation and hence activation of this kinase controls the expression of Hsp70 in these cells under thermal stress (Fig.…”

Exposure of gnotobiotic Artemia franciscana larvae to abiotic stress promotes heat shock protein 70 synthesis and enhances resistance to pathogenic Vibrio campbellii

“…Stress often stimulates Hsp expression in aquatic organisms with Hsp70 induced in hypoxic Nile tilapia Oreochromis niloticus (L.) juveniles (Delaney and Klesius 2004), Indian major carp, Cirrhinus mrigala (Ham. ; Das et al 2005), and the tiger prawn, P. monodon (de la Vega et al 2006). A temperature increase from 21 to 37°C for 30min followed by a 24-h recovery strongly induced Hsp70 in Artemia adults (Clegg et al 2000a), and a non-lethal heat shock from 28 to 37°C for 30min with a 6-h recovery triggered Hsp70 production in gnotobiotic-grown larvae (Sung et al 2007).…”