Heat Shock Protein 70 Expression is Increased in the Liver of Neonatal Intrauterine Growth Retardation Piglets

Li, Wei; Zhong, Xiang; Zhang, Lili; Wang, Yuanxiao; Wang, Tian

doi:10.5713/ajas.2012.12058

Cited by 10 publications

(11 citation statements)

References 38 publications

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“…The naturally-occurring IUGR pig was recognized as an ideal model for the study of human IUGR [ 2 ]. Based on our previous study, newborn IUGR piglets had fatty hepatic infiltrates and cytoplasmic vacuolization in the liver [ 3 ]. Investigation of the hepatic proteome also suggested that the IUGR fetus had a lower activity of lipoprotein lipase (LPL) [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Choline supplementation improves the lipid metabolism of intrauterine-growth-restricted pigs

et al. 2018

Asian-Australas J Anim Sci

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ObjectiveThe objective of this study was to investigate the effects of dietary choline supplementation on hepatic lipid metabolism and gene expression in finishing pigs with intrauterine growth retardation (IUGR).MethodsUsing a 2×2 factorial design, eight normal birth weight (NBW) and eight IUGR weaned pigs were fed either a basal diet (NBW pigs fed a basal diet, NC; IUGR pigs fed a basal diet, IC) or a diet supplemented with two times more choline than the basal diet (NBW pigs fed a high-choline diet, NH; IUGR pigs fed a high-choline diet, IH) until 200 d of age.ResultsThe results showed that the IUGR pigs had reduced body weight compared with the NBW pigs (p<0.05 from birth to d 120; p = 0.07 from d 120 to 200). Increased (p<0.05) free fatty acid (FFA) and triglyceride levels were observed in the IUGR pigs compared with the NBW pigs. Choline supplementation decreased (p<0.05) the levels of FFAs and triglycerides in the serum of the pigs. The activities of malate dehydrogenase and glucose 6-phosphate dehydrogenase were both increased (p<0.05) in the livers of the IUGR pigs. Choline supplementation decreased (p<0.05) malate dehydrogenase activity in the liver of the pigs. Gene expression of fatty acid synthase (FAS) was higher (p<0.05) in the IC group than in the other groups, and choline supplementation decreased (p<0.05) FAS and acetyl-CoA carboxylase α expression in the livers of the IUGR pigs. The expression of carnitine palmitoyl transferase 1A (CPT1A) was lower (p<0.05) in the IC group than in the other groups, and choline supplementation increased (p<0.05) the expression of CPT1A in the liver of the IUGR pigs and decreased (p<0.01) the expression of hormone-sensitive lipase in both types of pigs. The gene expression of phosphatidylethanolamine N-methyltransferase (PEMT) was higher (p<0.05) in the IC group than in the other groups, and choline supplementation significantly reduced (p<0.05) PEMT expression in the liver of the IUGR pigs.ConclusionIn conclusion, the lipid metabolism was abnormal in IUGR pigs, but the IUGR pigs consuming twice the normal level of choline had improved circulating lipid parameters, which could be related to the decreased activity of nicotinamide adenine dinucleotide phosphate-generating enzymes or the altered expressions of lipid metabolism-related genes.

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Section: Introductionmentioning

confidence: 99%

Choline supplementation improves the lipid metabolism of intrauterine-growth-restricted pigs

et al. 2018

Asian-Australas J Anim Sci

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“…In IUGR piglets, the higher susceptibility to infection or environmental changes could be due to an ineffective immune system and subnormal size and histopathology of the thymus (Cromi et al., ; Zhong et al., ). In IUGR piglets, an overexpression of heat shock protein 70 (a cytoprotective protein produced in response to different cellular stressor factors) has been found in association with impaired cellular immunity at the intestine and liver levels (Li, Zhong, Zhang, Wang, & Wang, ; Zhong, Wang, Zhang, & Li, ), which suggests an impaired immune function (Yuan et al., ).…”

Section: Intrauterine Growth Restriction (Iugr) Pigletsmentioning

confidence: 99%

The challenge of large litters on the immune system of the sow and the piglets

Oliviero

Junnikkala

Peltoniemi

2019

Reprod Domestic Animals

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The use of hyperprolific sow lines has increased litter size considerably in the last three decades. Nowadays, in some countries litters can reach up to 18–20 piglets being a major challenge for the sow's physiology during pregnancy, parturition and lactation. The increased number of piglets born per litter prolongs sensibly the duration of farrowing, decreases the piglets’ average weight at birth and their vitality, increases the competition for colostrum intake and can affect negatively piglets’ survival. This review aims to describe how large litters can affect the immune system of the sow and the piglets and proposes measures to improve this condition.

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“…Ekspresi Hsp-70 yang mengalami peningkatan pada janin tumbuh lambat diduga karena respons dari stres multipel. 5 Iskemia, hipoksia, dan peningkatan resistensi vaskular yang mengakibatkan kerusakan fungsi plasenta sebagian besar disebabkan oleh karena peningkatan stres oksidatif. Heat-shock protein-70 merupakan marker stres oksidatif, pada trofoblas ekspresinya meningkat pada kasus PJT dan preeklamsi dibandingkan dengan trofoblas hamil normal.…”

Section: 4unclassified

Ekspresi Human Leukocyte Antigen-G (HLA-G) dan Heat-Shock Protein-70 (Hsp-70) pada Pertumbuhan Janin Terhambat

Sulistyowati¹,

Eka²

2014

mkb

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AbstrakPertumbuhan janin terhambat (PJT) merupakan salah satu penyebab utama tingginya morbiditas dan mortalitas perinatal. Maladaptasi imun berakibat gangguan invasi trofoblas dan remodeling arteri spiralis yang akan menyebabkan hipoksia pada jaringan plasenta. Penelitian ini bertujuan untuk menganalisis ekspresi human leukocyte antigen-G (HLA-G) dan heat-shock protein-7 (Hsp-70) pada trofoblas PJT dan hamil normal, dengan menggunakan metode observasional analitik dan pendekatan potong lintang. Penelitian dilakukan di Bagian Obstetri dan Ginekologi Rumah Sakit Umum Daerah Dr. Moewardi Surakarta, mulai bulan November-Desember 2011. Jumlah sampel 30, terdiri atas 15 sampel trofoblas pada PJT dan 15 sampel trofoblas pada kehamilan normal. Pada semua sampel dilakukan pemeriksaan ekspresi HLA-G dan Hsp-70 dengan imunohistokimia. Analisis data menggunakan uji-t. Ekspresi HLA-G rata-rata pada kelompok PJT sebesar 32,42±8,90, sedangkan ekspresi HLA-G rata-rata pada kelompok kehamilan normal 43,92±14,91 (p=0,016). Ekspresi Hsp-70 pada kelompok PJT 2,4355+0,26647 dan kelompok kehamilan normal 1,5920+0,17142 (p=0,008). Simpulan, pada PJT ekspresi HLA-G lebih rendah dan ekspresi Hsp-70 lebih tinggi dibandingkan dengan kehamilan normal. [MKB. 2014;46(1):22-7] Kata kunci: HLA-G, Hsp-70, pertumbuhan janin terhambat Human Leukocyte Antigen-G (HLA-G) and Heat-Shock Protein-70 (Hsp-70) Expression on Intra Uterine Growth Retardation AbstractIntra uterine growth retardation (IUGR) is one of the leading causes of higher morbidity and mortality in perinatal.Immune maladaptation affects trophoblast invasion and spiralis arteria remodeling that will cause placental tissue hypoxia. This research aimed to analyze human leukocyte antigen-G (HLA-G) and heat-shock protein-70 (Hsp-70) expression on the IUGR trophoblast and normal pregnancy, by applying analytical observational method and cross sectional approach. This research was conducted at the Obstetric and Gynecology Department of Dr. Moewardi Hospital Surakarta from November to December 2011. The total samples were 30, divided into two groups. There were 15 samples trophoblast on IUGR and 15 samples trophoblast from normal pregnancy. All samples were tested for HLA-G and Hsp-70 using immunohistochemistry. The data were analyzed by using t-test. The mean of HLA-G expression on the IUGR groups was 32.42±8.90 and on the normal pregnancy groups was 43.92±14.91 (p=0.016). Heat-shock protein70 expression on the IUGR groups was 2.4355+0.26647 and on the normal pregnancy groups was 1.5920+0.17142 with p=0.008. In conclusion, in IUGR, the HLA-G expression is lower and the Hsp-70 expression is higher than in normal pregnancy. [MKB. 2014;46(1):22-7]

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Heat Shock Protein 70 Expression is Increased in the Liver of Neonatal Intrauterine Growth Retardation Piglets

Cited by 10 publications

References 38 publications

Choline supplementation improves the lipid metabolism of intrauterine-growth-restricted pigs

Choline supplementation improves the lipid metabolism of intrauterine-growth-restricted pigs

The challenge of large litters on the immune system of the sow and the piglets

Ekspresi Human Leukocyte Antigen-G (HLA-G) dan Heat-Shock Protein-70 (Hsp-70) pada Pertumbuhan Janin Terhambat

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