Heat shock protein-70 neutralizes apoptosis inducing factor in Bcr/Abl expressing cells

Wang, Fang; Dai, An-Ya; Tao, Kun; Xiao, Qing; Huang, Zhenglan; Gao, Miao; Li, Hui; Wang, Xin; Cao, Weixi; Feng, Wenli

doi:10.1016/j.cellsig.2015.06.006

Cited by 24 publications

(10 citation statements)

References 44 publications

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“…The expression of HSPs family specifically HSP70 increases under stress condition such as high temperature, chemotherapy, and radiation [25]. The important role of HSPs is chaperone function, transport and reparation, apoptosis, and protection of cells in stressful condition [26]. Another noteworthy finding of current study was the monitoring of HSP70 expression during designated experimental procedure which induced proportionally in response to induced cytotoxic effects.…”

Section: Discussionmentioning

confidence: 88%

Effects of combination of melatonin and laser irradiation on ovarian cancer cells and endothelial lineage viability

et al. 2016

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The main goal of anti-cancer therapeutic approaches is to induce apoptosis in tumor masses but not in the normal tissues. Nevertheless, the combination of photodynamic irradiation with complementary oncostatic agents contributes to better therapeutic performance. Here, we applied two different cell lines; SKOV3 ovarian carcinoma cells and HUVECs umbilical cord cells as in vitro models to pinpoint whether pharmacological concentration of melatonin in combination with photodynamic therapy induces cell cytotoxicity. The cells were separately treated with various concentrations of melatonin (0 to 10 mM) and photodynamic irradiation alone or in combination. Cells were preliminary exposed to increasing concentrations of melatonin for 24 h and subsequently underwent laser irradiation for 60 s with an output power of 80 mW in continuous mode at 675 nm wavelength and a total light dose of 13.22 J/cm. Cell viability, apoptosis/necrosis rates, and reactive oxygen species levels as well as heat shock protein 70 expression were monitored after single and combined treatments. A statistical analysis was performed by applying one-way analysis of variance (ANOVA) and post hoc Tukey's test. Combination treatment of both cell lines caused a marked increase in apoptosis/necrosis rate, reactive oxygen species generation, and heat shock protein 70 expression compared to incubation of the cells with each agent alone (p < 0.05). SKOV3 cancer cells expressed higher level of heat shock protein 70 under experimental procedure as compared to HUVECs (p < 0.05). Our results introduce melatonin as a potent stimulus for enhancing the efficacy of laser on induction of apoptosis in tumor cells.

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Section: Discussionmentioning

confidence: 88%

Effects of combination of melatonin and laser irradiation on ovarian cancer cells and endothelial lineage viability

et al. 2016

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“…This finding implied that altered expression of MFN2 induced by stress was related to concomitant alterations in mitochondrial function. In addition, overexpression of MFN2 induces cell apoptosis (Wang et al ., ,b; Zhang et al ., ), which is consistent with our finding of an elevated sperm apoptosis rate. Taken together, the increased expression of MFN2 in our study may reflect the heat‐induced alteration of sperm mitochondrial functioning and metabolism, and increased MFN2 may also participated in heat‐induced sperm apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Heat‐shock proteins are capable of protecting cells from harmful stress and play important roles in the regulation of cell apoptosis. (Park et al ., ; Wang et al ., ,b). It is noteworthy that most proteins in spermatozoa were formed before spermatid DNA condensation, and mature spermatozoa exhibit almost no protein synthesis.…”

Section: Discussionmentioning

confidence: 99%

Transient scrotal hyperthermia affects human sperm DNA integrity, sperm apoptosis, and sperm protein expression

et al. 2016

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This prospective randomized clinical study is aimed to evidence the reproductive impairment of frequent scrotal heat exposure. A total of 20 normozoospermic subjects were randomly divided into two groups to undergo testicular warming in a 43 °C water bath 10 times, for 30 min each time; the subjects in group 1 underwent testicular warming for 10 consecutive days and those in group 2 once every 3 days. Sperm chromatin structure assay (SCSA), sperm mitochondrial membrane potential (MMP), apoptosis, and seminal plasma-soluble Fas (sFas) were analyzed before treatment and every 2 weeks after, for a total of 10 times. In group 1, some critical proteins involved in heat stress, hypoxia, structure, and function of sperm mitochondria and flagella were evaluated before hyperthermia and 2, 6, 10, and 16 weeks after hyperthermia. Both groups showed a reversible increase in the proportion of spermatozoa with a disrupted MMP (both p < 0.05 when the minimums were compared with baseline levels, the same below), sperm apoptosis (both p < 0.01) and high DNA stainability (both p < 0.05). The sFas concentration in both groups showed no obvious changes except one: the value at week 2 was significantly increased over baseline in group 1 (p = 0.036). The level of Bcl-2 decreased significantly at weeks 6 and 10 (p = 0.017 and 0.05, respectively) and recovered to baseline at week 16. Proteins involved in heat stress and mitochondria functions were up-regulated, whereas in flagella structure and function was down-regulated (all p < 0.05). This study demonstrated that transient and frequent scrotal hyperthermia severely and reversibly damaged spermatogenesis, consecutive heat exposure had more serious effects than intermittent exposure, whereas intermittent exposure led to a later recovery of sperm damage.

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“…The heat shock response (HSR) is known to be a possible cellular technique which may affect the upper thermal tolerance of any organism (Lee et al, 2016). The HSR is recognized by the ability to synthesize many protein types, Heat Shock Proteins (HSPs), establishing and refolding proteins in addition to leading formerly denatured proteins to the proteolytic machinery (Tomanek, 2010;Roberts et al, 2010;Wang et al, 2015;Zunino et al, 2016). In contrary to the HSR, that gave rise to transient stimulation of HSPs as a result of any unexpected heat shock, acclimation reaction refers to physiological variations that presented if the organism suffered from temperature changes for a long period (Lewis et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Temperature changes and marine fish species (Epinephelus coioides and Sparus aurata): Role of oxidative stress biomarkers in toxicological food studies

et al. 2018

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The increase of seawater temperature as a result of global climate variation elucidates a major challenge for marine organisms survival in addition to consumers safety. Spotted grouper (Epinephelus coioides) and Seabream (Sparus aurata) were collected in water with different temperature variations at Suez Canal and Alexandria (Suez and Abu Qir bay) in Egypt with the aim to assess expression levels of heat shock proteins such as HSP47, HSP70 and HSP90 genes in addition to antioxidants value through enzymes activity: Glutathione-S-Transferase (GST) and Glutathione Peroxidase (GPx). Research results revealed that expression of the HSP47, HSP70a and HSP90 genes increased in marine fishes tissues collected from Suez Canal, with higher water temperature (23:28ºC), compared with those collected from Alexandria (19:24°C) whereas the content of GPx and GST decreased. Our results show alteration of the marker examined suggesting that the increase of heat shock protein genes expression levels of fish collected from Suez Canal might be exposed mainly to thermal oxidative stress response more than those collect from Alexandria. The increase of heat shock protein-related genes expression could be considered as a factor in prohibiting the heat shock transcription factor that may lead to stimulation of heat-inducible genes in addition to heat acclimation. Thus, warming of water is also likely to alter the composition and abundance of food resources, e.g. fish muscles, available to higher trophic level consumers.

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Heat shock protein-70 neutralizes apoptosis inducing factor in Bcr/Abl expressing cells

Cited by 24 publications

References 44 publications

Effects of combination of melatonin and laser irradiation on ovarian cancer cells and endothelial lineage viability

Effects of combination of melatonin and laser irradiation on ovarian cancer cells and endothelial lineage viability

Transient scrotal hyperthermia affects human sperm DNA integrity, sperm apoptosis, and sperm protein expression

Temperature changes and marine fish species (Epinephelus coioides and Sparus aurata): Role of oxidative stress biomarkers in toxicological food studies

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