Heat shock protein 70 purification and characterization from Cyprinion macrastomus macrastomus and Garra rufa obtusa

Tutar, Yusuf; Okan, Şule

doi:10.1016/j.jtherbio.2011.11.002

Cited by 13 publications

(4 citation statements)

References 26 publications

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“…We therefore studied the association of elevated HSPs with oxidative stress markers by utilizing a physiological model of thermal stress adaptation in Garra rufa, acclimatized to different thermal conditions. The presence of a functional HSP system in this fish species has been recently confirmed [7] ; thus it was possible to evaluate the relationships between HSPs and oxidative stress by comparing populations of Garra rufa found in elevated water temperature in Kangal hot spring to those found in normal river water temperature. We sought evidence whether sustained elevation of HSPs can decrease oxidative stress and enhance cytoprotection.…”

Section: Introductionmentioning

confidence: 80%

Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress

et al. 2014

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Heat shock proteins (HSPs), originally identified as heat-inducible gene products, are a family of highly conserved proteins that respond to a wide variety of stress including oxidative stress. Although both acute and chronic oxidative stress have been well demonstrated to induce HSP responses, little evidence is available whether increased HSP levels provide enhanced protection against oxidative stress under elevated yet sublethal temperatures. We studied relationships between oxidative stress and HSPs in a physiological model by using Garra rufa (doctor fish), a fish species naturally acclimatized to different thermal conditions. We compared fish naturally living in a hot spring with relatively high water temperature (34.4±0.6 °C) to those living in normal river water temperature (25.4±4.7 °C), and found that levels of all the studied HSPs (HSP70, HSP60, HSP90, HSC70 and GRP75) were higher in fish living in elevated water temperature compared with normal river water temperature. In contrast, indicators of oxidative stress, including protein carbonyls and lipid hydroperoxides, were decreased in fish living in the elevated temperature, indicating that HSP levels are inversely associated with oxidative stress. The present results provide evidence that physiologically increased HSP levels provide protection against oxidative stress and enhance cytoprotection.

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Section: Introductionmentioning

confidence: 80%

Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress

et al. 2014

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“…; Chapuis et al . ; Tutar & Okan ). We interpret these findings to say that broad generalizations about pest insects relative to climate change effects may be premature, and more data on individual species are necessary.…”

Section: Introductionmentioning

confidence: 98%

“…For example, the insecticide propoxur increased thermotolerance in the mosquitoes Anopheles stephellsi Liston and Aedes aegypti (L.) (Patil et al 1996). Molecular mechanisms of thermal resistance are mainly related to up-regulation of cell-protecting proteins, including heatshock proteins (Hsps) (Arias et al 2011;Chapuis et al 2011;Tutar & Okan 2012). We interpret these findings to say that broad generalizations about pest insects relative to climate change effects may be premature, and more data on individual species are necessary.…”

Section: Introductionmentioning

confidence: 99%

Molecular basis for insecticide‐enhanced thermotolerance in the brown planthopper Nilaparvata lugens Stål (Hemiptera:Delphacidae)

Huang

Gao

et al. 2013

Molecular Ecology

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Climate change is likely to have marked ecological effects on terrestrial ecosystems, including the activities of insect pests. Most attention has focused on the increasing geographical ranges of pests; however, if extrinsic factors enhance their thermotolerance, populations may express increased voltinism and longer daily and annual activity periods. These changes in pest populations have the potential for severe consequences, including increased crop losses and decreased food security at the global level. The brown planthopper (BPH) Nilaparvata lugens Stål (Hemiptera: Delphacidae) is a serious pest of rice crops in temperate and tropical regions of Asia. It is often present in rice microclimates at temperatures close to its maximum thermotolerance. Recent BPH outbreaks in tropical Asia are considered to be associated with excess use of pesticides and increasing temperature. This study tested whether exposure to sublethal concentrations of triazophos (tzp), an insecticide widely used in Asian rice production, enhances thermotolerance of BPH. Tzp exposure (40 ppm at 40 °C) significantly decreased mortality (from 94% in controls to 50% at 48 h post-treatment) and increased lethal mean time (LT50 ) of adults by 17.2 h. To investigate the underlying molecular mechanism of this tzp-enhanced thermotolerance, we selected Hsp70 and Arginine kinase (Argk) for detailed study. Transcripts encoding both proteins in third-instar nymphs and brachypterous adult females were up-regulated, compared with controls, after exposure to tzp. RNAi silencing of both genes demonstrated that Hsp70 and Argk are essential for survival and tzp-increased thermotolerance. We propose that tzp induces thermotolerance in BPHs by increasing the expression of genes that act in cell protection mechanisms. The significance of our proposal relates to the importance of understanding the influence of sublethal concentrations of insecticides on pest biology. In addition to its influence on thermotolerance, tzp also enhances BPH reproduction. We infer that exposure to a pesticide stressor can produce cross-tolerance, that is, increased tolerance to one stressor also increases tolerance to other stressors, including temperature. Aside from needing a better understanding of these effects in nature and in other pest/cropping systems, we suggest that pest management programmes can be improved with better understanding of the influences of stressors, including increased environmental temperatures and sublethal concentrations of insecticides, on pest biology.

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“…Another marker exhibiting an increase under stress heat shock protein HSP70. The increase occurring in HSP70 offers protection for cell culture and animal tissues (25). Also, HSP70 helps proteins to be transported in cells and has a function in the folding of proteins synthesized within the cell and it is responsible for the destruction of unstable proteins as well as preventing protein aggregation and it also helps protein folding refold when damaged and apoptosis (26).…”

Section: Introductionmentioning

confidence: 99%

Effects of HBV and HCV infection on oxidative stress and inflammation in hemodialysis patients

et al. 2018

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Background and purpose: Chronic renal failure is a syndrome characterized by progressive and irreversible loss of nephrons depending on various diseases. Especially infection such as HBV and HCV is among the major causes of mortality and morbidity in hemodialysis patients.In the present study we aimed to determine the levels of 3-nitrotyrosine (3-NT), TNF-like weak inducer of apoptosis (TWEAK) and heat shock protein (HSP70) in chronic renal failure patients diagnosed with HBV, HCV and non-hepatitis undergoing hemodialysis treatment. Materials and methods: Samples of 235-patients receiving hemodialysis treatment and 25-healthy individuals were included in the study. Firstly, HBV and HCV positivity were diagnosed by serological Enzyme-Linked ImmunuSorbent Assay (ELISA) method. Then, 3-NT levels were determined using High-Performance Liquid Chromatography (HPLC), while TWEAK and HSP70 were determined using high sensitivity ELISA. The numbers of patients used in the studies were determined according to statistical power analysis. Results: The values of 3-NT and HSP70 were found to be significantly higher non-hepatitis patients, HBV and HCV patients receiving hemodialysis treatment to compared with the control group (p‹0.05). Concentration of TWEAK in non-hepatitis and HBV patients receiving hemodialysis therapy was found to be significantly higher to compared with the control group (p‹0.05). However, unlike the other two groups, an increase in the TWEAK of HCV patients on post-dialysis was determined. Conclusions: To prevent the increase of inflammation and oxidative stress in hemodialysis patients or to keep it under control, investigating certain parameters such as TWEAK, 3-NT and HSP70 from time to time is of great importance in terms of minimizing the level of risk for many diseases such as cardiovascular diseases. Infection also increases the burden on oxidative stress and immunity system in these patients.

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Heat shock protein 70 purification and characterization from Cyprinion macrastomus macrastomus and Garra rufa obtusa

Cited by 13 publications

References 26 publications

Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress

Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress

Molecular basis for insecticide‐enhanced thermotolerance in the brown planthopper Nilaparvata lugens Stål (Hemiptera:Delphacidae)

Effects of HBV and HCV infection on oxidative stress and inflammation in hemodialysis patients

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