Heat Shock Protein Expression During Gametogenesis and Embryogenesis

Neuer, A.; Spandorfer, S.D.; Giraldo, Paulo César; Jeremias, Jan; Dieterle, Stefan; Korneeva, Irina; Liu, H.-C.; Rosenwaks, Zev; Witkin, Steven S.

doi:10.1155/s1064744999000034

Cited by 45 publications

(21 citation statements)

References 38 publications

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“…Heat shock proteins are stress proteins involved in facilitating protein folding and assembly and in stabilizing the damaged protein for repair and degradation in cells [41], and they may protect the embryo from undergoing apoptosis [42]. Culture of embryos with antibody against HSP70 is associated with more DNA fragmentation and growth inhibition of the treated embryos [42,43]. Endometrial cell coculture reduces these detrimental effects of anti-HSP antibody [44].…”

Section: Discussionmentioning

confidence: 99%

Embryotrophic Factor-3 from Human Oviductal Cells Affects the Messenger RNA Expression of Mouse Blastocyst1

Lee

et al. 2003

Biology of Reproduction

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Our previous results showed that embryotrophic factor-3 (ETF-3) from human oviductal cells increased the size and hatching rate of mouse blastocysts in vitro. The present study investigated the production of ETF-3 by an immortalized human oviductal cell line (OE-E6/E7) and the effects of ETF-3 on the mRNA expression of mouse embryos. The ETF-3 was purified from primary oviductal cell conditioned media using sequential liquid chromatographic systems, and antiserum against ETF-3 was raised. The ETF-3-supplemented Chatot-Ziomek-Bavister medium was used to culture Day 1 MF1 x BALB/c mouse embryos for 4 days. The ETF-3 treatment significantly enhanced the mouse embryo blastulation and hatching rate. The antiserum, at concentrations of 0.03-3%, abolished the embryotrophic effect of ETF-3. Positive ETF-3 immunoreactivity was detected in the primary oviductal cells, OE-E6/E7, and blastocysts derived from ETF-3 treatment. Vero cells (African Green Monkey kidney cell line), fibroblasts, and embryos cultured in control medium did not possess ETF-3 immunoreactivity. The mRNA expression patterns of the treated embryos were studied at the blastocyst stage by mRNA differential display reverse transcription-polymerase chain reaction (DDRT-PCR). The DDRT-PCR showed that some of the mRNAs were differentially expressed after ETF-3 treatment. Twelve of the differentially expressed mRNAs that had high homology with cDNA sequences in the GenBank were selected for further characterization. The differential expression of seven of these mRNAs (ezrin, heat shock 70-kDa protein, cytochrome c oxidase subunit VIIa-L precursor, proteinase-activated receptor 2, eukaryotic translation initiation factor 2beta, cullin 1, and proliferating cell nuclear antigen) was confirmed by semiquantitative RT-PCR. In conclusion, immortalized oviductal cells produce ETF-3, which influences mRNA expression of mouse blastocyst.

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Section: Discussionmentioning

confidence: 99%

Embryotrophic Factor-3 from Human Oviductal Cells Affects the Messenger RNA Expression of Mouse Blastocyst1

Lee

et al. 2003

Biology of Reproduction

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“…; Neuer et al. ; Yang and Rajamahendran ). In particular, mRNA expression levels of the pro‐apoptotic gene BAX and the anti‐apoptotic gene BCL2 are considered an indicator of blastocyst quality in bovine pre‐implantation embryos (Yang and Rajamahendran ).…”

Section: Introductionmentioning

confidence: 99%

Effect of Bovine Follicular Fluid on Reactive Oxygen Species and Glutathione in Oocytes, Apoptosis and Apoptosis‐related Gene Expression ofIn vitro‐Produced Blastocysts

Park

2014

Reprod Domestic Animals

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The reactive oxygen species (ROS) generated during the in vitro maturation of oocytes affect oocyte maturation and subsequent embryonic development. Bovine follicular fluid (bFF) has an effective antioxidant capacity. This study was conducted to investigate the effects of supplementing oocyte maturation media with bFF from different size classes (3-8 and 9-13 mm) on the glutathione (GSH) and ROS levels of oocytes. Embryonic development and apoptosis, as well as the relative abundance of INFτ, BAX, BCL2 and HSP70 transcripts in blastocysts, were also monitored. Oocytes collected from ovaries were matured in TCM-199 with FBS (control) and 10% 3-8 mm (M), 9-13 mm (L) or a mixture of 3-8 mm and 9-13 mm (M + L) bFF. Glutathione and ROS levels in oocytes after 24 h were assessed by Cell Tracker Blue CMF2HC and DCHFDA staining, respectively. Apoptosis in day-8 blastocysts was assessed by TUNEL staining. The relative abundance of BAX, BCL2, HSP70 and INFτ transcripts was assessed using quantitative real-time polymerase chain reaction (PCR). The GSH level was significantly higher in the L group compared to the other groups (p < 0.05), while the ROS levels in the M group were significantly higher than in the other groups (p < 0.05). The apoptosis levels of blastocysts in the FBS group were significantly higher than those in the M + L group (p < 0.05), although the embryonic development did not differ between the groups. The HSP70 and INFτ expression levels in group M were significantly greater than in the controls (p < 0.05). There was no significant difference in BAX expression between the groups. Supplementation with bFF from various sizes of follicles into the maturation medium was capable of supporting oocyte cytoplasmic maturation by decreasing the ROS. Moreover, bFF subsequently affected antioxidative gene expression, increasing HSP70 and INFτ expressions.

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“…Heat shock influences reproduction in many insect species (Neuer et al, 1999(Neuer et al, , 2000Gruntenko et al, 2003. Male sterility at extreme temperature is a common phenomenon and the thermal thresholds leading to sterility vary across species and lines within species.…”

Section: Introductionmentioning

confidence: 99%

Effects of heat shock on ovary development and hsp83 expression in Tribolium castaneum (Coleoptera: Tenebrionidae)

Shen

Qiu

et al. 2009

Arch Insect Biochem Physiol

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Heat shock affects reproductive performance in insects including Tribolium castaneum. In this study, the effects of heat shock on ovary development and hsp83 expression in T. castaneum were investigated. Two lines of T. castaneum, H line and C line, from the same base population were established and maintained for five successive generations. In each generation, the newly hatched beetles (within 3 h after eclosion) in the H line were treated with a heat shock at 40 degrees C for 1 h, and those in the C line were raised at normal temperature (30 degrees C) as control treatment. Four traits related to ovary development were measured for the beetles of the fifth generation: days from eclosion to laying the first eggs (T(o)), days from eclosion to laying the first hatchable eggs (T(h)), ovariole size on the third day after eclosion, and pupal mass of their offspring. The results showed that the beetles of the H line had a significantly longer pre-oviposition period (0.6 more days) and smaller ovariole size than those of the C line. No significant difference in pupal mass was observed. Applying heat shock to the offspring of the fifth generation of both lines led to significantly higher hsp83 expression in offspring of the C line than in offspring of the H line. Within each line, the hsp83 expression level in offspring with heat shock was significantly higher than that of offspring without heat shock, but the difference in the C line was much larger than that in the H line. We infer from these results that a tradeoff between heat resistance, registered as hsp83 expression, and ovarian development operates under heat stress in T. castaneum.

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Heat Shock Protein Expression During Gametogenesis and Embryogenesis

Cited by 45 publications

References 38 publications

Embryotrophic Factor-3 from Human Oviductal Cells Affects the Messenger RNA Expression of Mouse Blastocyst1

Embryotrophic Factor-3 from Human Oviductal Cells Affects the Messenger RNA Expression of Mouse Blastocyst1

Effect of Bovine Follicular Fluid on Reactive Oxygen Species and Glutathione in Oocytes, Apoptosis and Apoptosis‐related Gene Expression ofIn vitro‐Produced Blastocysts

Effects of heat shock on ovary development and hsp83 expression in Tribolium castaneum (Coleoptera: Tenebrionidae)

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