Heat shock protein expression in canine malignant mammary tumours

Romanucci, Mariarita; Marinelli, Alessia; Sarli, Giuseppe; Salda, Leonardo Della

doi:10.1186/1471-2407-6-171

Cited by 44 publications

(48 citation statements)

References 67 publications

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“…Similar studies are conducted in veterinary medicine, but to a lesser extent (Isomoto et al, 2003). A similar study was conducted by Rommanucci (2005) in mammary gland tumors in female dogs (Romanucci et al, 2006). She found Hsp70 and Hsp90 expression in simple and complex, as well as solid-type adenocarcinomas.…”

Section: Discusionsupporting

confidence: 64%

“…Paraffin sections on slides covered with 2% saline solution in acetone, at the temperature of 42 0 C were used for immunohistochemical methods. In immunohistochemical reactions, the following antibodies, properly diluted in 1% BSA (Sigma), were used: mouse monoclonal antibody against human nuclear antigen Ki-67 (Dako) diluted 1:75 (Nieto et al, 2000), mouse monoclonal antibodies against p53 (Dako) human protein, diluted at a ratio 1:25 (Gamblin et al, 1994;Rodo, 2007), mouse monoclonal antibodies against alpha (Dako) human estrogen receptor, diluted at a ratio 1:35 (Mulas et al, 2005), mouse monoclonal antibodies against COX-2 (Dako) human receptor, diluted at a ratio 1:100 (Queiroga et al, 2007;Doré et al, 2003;Soslow et al, 2000), mouse monoclonal antibodies against Hsp70 (Novocastra) human heat shock proteins, diluted at a ratio 1:40 (Romanucci et al, 2006), mouse monoclonal antibodies against Hsp90 (Novocastra) human heat shock proteins, diluted at a ratio 1:40 (Romanucci et al, 2006 ) and mouse monoclonal antibodies against P-glycoprotein (Sigma), diluted at a ratio 1:100 (Petterino et al, 2006). Sections were deparafinized in xylene and rehydrated in increasing concentrations of alcohol.…”

Section: Methodsmentioning

confidence: 99%

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12_Prospective Study of Tumor Markers as Prognostic Factors in the Histopathological Differential Diagnosis of Mammary Gland Neoplasms in Female Canines

Badowska-Kozakiewicz¹

2012

A Bird's-Eye View of Veterinary Medicine

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Section: Discusionsupporting

confidence: 64%

Section: Methodsmentioning

confidence: 99%

12_Prospective Study of Tumor Markers as Prognostic Factors in the Histopathological Differential Diagnosis of Mammary Gland Neoplasms in Female Canines

Badowska-Kozakiewicz¹

2012

A Bird's-Eye View of Veterinary Medicine

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“…Thus, HSP60 together with their peptides could somehow induce a better response of lymphocytes to CMT. On the other hand, other HSP types, such as HSP27, HSP72 and HSP90 were studied in mammary carcinomas in bitches and presenting results that indicate the association of these proteins with the carcinogenesis process [22]. However, more studies are needed to understand the involvement of HSP60 in CMT.…”

Section: Discussionmentioning

confidence: 99%

“…The heat shock proteins (HSP) are a large family of molecular chaperon proteins [5]. Increased HSP in damaged cells may also aid in cell integrity, since it inhibits the apoptosis [6], and overexpression of the different proteins of this group is related to a poorer prognosis of the patient, including MMT [22].…”

Section: Introductionmentioning

confidence: 99%

CD4+, CD8+, FoxP3+ and HSP60+ Expressions in Cellular Infiltrate of Canine Mammary Carcinoma in Mixed Tumor

Lopes-Neto

Souza

Bouty

et al. 2017

Acta Scientiae. Vet.

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Background: Cancer is a complex process that receive many influences of the tumor microenvironment. The participation of immune system cells and proteins in tumor microenvironment is not yet completely understood. Thus, the aim of this study was to evaluate the infiltrate cellular, subpopulations of T-lymphocytes and HSP60 of canine mammary carcinoma in mixed tumor (CMCMT). Materials, Methods & Results:Female dogs (n = 20) were selected after Canine mammary tumor (CMT) diagnosis and data were achieved throughout clinical-pathological information. Clinical staging was evaluated and tumor biopsies were processed by histology and cellular infiltrate was performed according criteria and grade. Survival curve were generated by Kaplan-Meier and the lymphocytic infiltrate were compared by Log-Rank followed Chi-Square χ². For immunolabeling it was used anti-CD4, anti-CD8, anti-FoxP3 and HSP60 monoclonal antibodies and were attributed scores from 0 to 3. Clinical-pathological relationship was analyzed using Spearman correlation. This study was approved by the Committee for Ethics in Research using Animals (CEUA-UECE), protocol 12247080-2. Our data showed a mean age of 9.3 years-old, the size of tumors presented more than 5 cm (50%), which were located in inguinal mammary glands (70%), and CMTs shows I (70%) and II (30%) grade. The cellular infiltrate was distributed both in peri and intratumoral regions, dispersed multifocally with moderate intensity and lymphocytes were the major populations found into tumors (n = 826 ± 220). In relationship to cellular infiltrate with CMT grade it was observed that lymphocytes (ρ = 0.28) and plasma cells (ρ = 0.22) showed a slight positive correlation, and an opposed negative correlation of neutrophils (ρ = -0.1) and macrophages (ρ = -0.38). CMT presents moderate lymphocytic infiltrate (< 800 lymphocytes), shows higher (P = 0.01) survival rates as compared to intense lymphocytic infiltrate (≥ 800 lymphocytes). FoxP3+ showed lower intensity while CD4+ and CD8+ expression were concentrated surrounding of lymphocytic infiltrate tumor region. HSP60+ was observed in the inflammatory and tumor cells. Discussion: Our data are according to a greater risk to the development of breast tumor in old bitches, not castrated and before or after puberty, as well as the use of contraceptives based on progesterone and estrogen. In relation to size of tumor, these findings reinforce that there is a relationship of tumor size with a higher malignancy grade and with a worse prognosis. The predominant tumor location was in the inguinal breasts that is attributed to the high activity of the mammary glands to hormonal stimuli. CMT with low clinical staging are associated with greater overall survival of affected bitches. In relation to tumor microenvironment, it has been reported that heterogeneous populations of the immune system cells often infiltrate the mammary tumors, whose lymphocytes are the main cells. It is suggested that tumor lymphocytosis may be necessary for malignant behavior of the tumo...

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“…In dogs, the expression of heat shock proteins was found in squamous cell carcinoma of the skin (Romanucci et al 2005, Bongiovanni et al 2008, cervical cancer (Chu et al 2001) and mammary gland tumors (Kumaraguruparan et al 2006, Romanucci et al 2006. Romanucci (2006) demonstrated the expression of Hsp90 in all examined tumors (simple and complex carcinomas) and found that these proteins are associated with poor outcome and are involved in carcinogenesis in the same way as heat shock protein 27. Available published data (Chen et al 1999) show that in breast cancer cells, the overexpression of heat shock protein 70 is also associated with poor prognosis.…”

Section: Introductionmentioning

confidence: 93%

Immunohistochemical evaluation of expression of heat shock proteins HSP70 and HSP90 in mammary gland neoplasms in bitches

Badowska-Kozakiewicz¹,

Malicka²

2012

Polish Journal of Veterinary Sciences

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Heat shock proteins have essential roles in a number of pathophysiologic conditions including carcinogenesis and represent a group of novel molecular markers in cancer management.The aim of this study was to investigate heat shock protein expression in correlation with other neoplasm traits such as: histological type, differentiation grade, proliferative activity, estrogenic receptor expression, and cyclooxygenase-2 and p53 proteins. Material for the investigation comprised 133 tumors of the mammary gland collected from bitches. In total 14 adenomas, 66 complex carcinomas, 47 simple carcinomas and 6 solid carcinomas were collected. Evaluations were conducted with histopathological and immunohistochemical methods using suitable antibodies. Expression of heat shock protein 70 was observed in all types of evaluated neoplasms. A higher average number of cells undergoing expression of heat shock protein 70, which was statistically insignificant, was established in complex and simple cancers and in cancers with the 1 st and the 2 nd degree of histological malignancy. Expression of heat shock protein 90 was observed in all studied neoplasms; it was very insignificant in adenomas, compared to cancers, and the highest expression was established in the solid cancers, as well as in cancers with the 2 nd degree of histological malignancy. This high expression of heat shock protein 90 was correlated with proliferative activity. The results suggest that heat shock protein 90 is involved in canine mammary gland carcinogenesis. The results also suggest that heat shock protein 90 may be a prognostic factor, but this requires detailed clinical confirmation.

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Heat shock protein expression in canine malignant mammary tumours

Cited by 44 publications

References 67 publications

12_Prospective Study of Tumor Markers as Prognostic Factors in the Histopathological Differential Diagnosis of Mammary Gland Neoplasms in Female Canines

12_Prospective Study of Tumor Markers as Prognostic Factors in the Histopathological Differential Diagnosis of Mammary Gland Neoplasms in Female Canines

CD4+, CD8+, FoxP3+ and HSP60+ Expressions in Cellular Infiltrate of Canine Mammary Carcinoma in Mixed Tumor

Immunohistochemical evaluation of expression of heat shock proteins HSP70 and HSP90 in mammary gland neoplasms in bitches

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