Heat shock protein (HSP) 47 and collagen are upregulated during neointimal formation in the balloon-injured rat carotid artery

Murakami, Shigeru; Toda, Yoshihisa; Seki, Takayuki; Munetomo, Eiji; Kondo, Yukiko; Sakurai, Takanobu; Furukawa, Yôko; Matsuyama, Mototaka; Nagate, Takatoshi; Hosokawa, Nobuko; Nagata, Kazuhiro

doi:10.1016/s0021-9150(00)00743-7

Cited by 29 publications

(22 citation statements)

References 30 publications

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“…7 In contrast, following balloon injury to rat carotid arteries, HSP47 mRNA increased for up to 7 days after injury before declining to control levels at 14 days, while the abundance of HSP47-positive cells in the expanding neointima increased between days 7 and 14. 30 Observations made using in situ hybridization in carbon tetrachloride-treated rat livers are also relevant to this question. While the abundance of HSP47 transcripts and HSP47-expressing cells increased throughout the period of CCl 4 administration, HSP47 mRNA-expressing cells actually decreased in areas of 'completed' fibrosis at 12 weeks of CCl 4 treatment.…”

Section: Discussionmentioning

confidence: 99%

Expression of HSP47, a collagen-specific chaperone, in normal and diseased human liver

Brown

Broadhurst

Mathahs

et al. 2005

Laboratory Investigation

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HSP47 is a collagen-specific chaperone that is required for normal collagen synthesis. In animal models of liver injury, hepatic stellate cells (HSC) have been identified as a source of HSP47. Because expression of HSP47 has not been investigated in human liver, the aim of these studies was to characterize expression of HSP47 in human liver and to investigate its regulation in human HSC in vitro. Immunohistochemistry demonstrated staining for HSP47 along the sinusoids of normal and cirrhotic human livers and in fibrous septa. Dual fluorescence confocal microscopy showed colocalization of HSP47 with synaptophysin, a marker for HSC. Levels of immunoreactive HSP47 and its transcript tended to be higher in cirrhotic livers than in normal livers. The abundance of HSP47 protein was unchanged by treatment of cultured human HSC with TGF-b1, angiotensin II, hypoxia and a number of other treatments intended to increase collagen synthesis. A modest reduction in HSP47 was achieved by transfection with antisense oligonucleotides and was associated with a significant decrease in procollagen synthesis. These observations suggest that HSP47 is constitutively expressed in human HSC and that HSP47 may be a target for antifibrotic therapy.

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Section: Discussionmentioning

confidence: 99%

Expression of HSP47, a collagen-specific chaperone, in normal and diseased human liver

Brown

Broadhurst

Mathahs

et al. 2005

Laboratory Investigation

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“…The spatiotemporally concerted expression of HSP47 with collagens is also observed during mouse and chick embryonic development (10). In addition, a marked induction of HSP47 has been reported in the progression of fibrosis in various experimental fibrosis models, including liver cirrhosis (11), kidney fibrosis (12), and atherosclerosis (13). The down-regulation of hsp47 caused by introducing hsp47 antisense oligoribonucleotides into rat renal cells after the initiation of fibrosis markedly reduces the rate of the progression of fibrosis and reduces the levels of types I and III collagens in the kidney (14).…”

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confidence: 95%

The Kruppel-like Factor Zf9 and Proteins in the Sp1 Family Regulate the Expression of HSP47, a Collagen-specific Molecular Chaperone

Yasuda¹,

Hirayoshi²,

Hirata³

et al. 2002

Journal of Biological Chemistry

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In several cells and tissues the synthesis of HSP47, a collagen-specific molecular chaperone in the endoplasmic reticulum, is closely correlated with the synthesis of collagen. We previously reported that the Sp1 binding site at ؊210 bp in the promoter region and the first and second introns are required for the tissue-specific expression of HSP47 in transgenic mice (Hirata, H., Yamamura, I., Yasuda, K., Kobayashi, A., Tada, N., Suzuki, M., Hirayoshi, K., Hosokawa, N., and Nagata, K. (

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“…23,24 Pre vi o us stu di es ha ve shown a mar ked in cre a se in HSP47 ex pres si on in pa ral lel with in cre a sed col la gen pro duc ti on and type I procol la gen ex pres si on du ring the prog res si on of at he rosc le ro sis in bal lo on-in ju red rat and rab bit ca ro tid ar te ri es, and in at he ro ma to us pla qu es of hu man co ro nary ar te ri es. 5,25,26 All the se stu di es re ve a led the ma jor ro les of HSP47 and col la gen ex-Reel ve ark.…”

Section: Discussionmentioning

confidence: 99%

“…12 In de ed, pre vi o us stu di es has shown that HSP47 and col la gen ha ve es sen ti al ro les in ad vanced at he rosc le ro tic le si ons. 5 Ho we ver, the exact natu re of the ir ro les and pos sib le in te rac ti ons ha ve not be en un ders to od in the early at he rosc le ro tic pro cess. On the ot her hand, the deg ra da ti on of newly synthe si sed pro col la gen and ma tu re col lagen is pri ma rily car ri ed out by matrix metalloproteinases (MMPs).…”

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confidence: 99%

“…4 HSPs ha ve be en shown to be highly ex pres sed in car di o vas cu lar tis su es, and indu ce inf lam ma tory res pon ses and prog res si on of athe rosc le ro sis. 5,6 HSP47, a stress-in du cib le pro te in lo cated in the en dop las mic re ti cu lum, acts as a mole cu lar cha pe ro ne and par ti ci pa tes in the in tra cellu lar pro ces sing, fol ding as sembly and sec re ti on of pro col la gens. 7,8 Col la gen is the ma jor cons ti tu ent of extracellular matrix (ECM) in ar te ri es and myo car di um.…”

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confidence: 99%

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Endothelin Receptor-Independent Correlation Between HSP47 and Collagen in Rabbit Model of Early Atherosclerosis

Reel¹,

Cavdar²,

Ergür³

et al. 2011

Turkiye Klinikleri J Med Sci

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A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : Col la gen in the ex tra cel lu lar mat rix (ECM) plays an im por tant ro le in mo dula tion of res pon se to the vas cu lar in jury du ring the prog res si on of at he rosc le ro sis and res te no sis. Colla gen can re gu la te smo oth musc le cell (SMC) pro li fe ra ti on, mig ra ti on and mat rix me tal lop ro te i na se (MMP) pro duc ti on. The re fo re, col la gen tur no ver in the ar te ri es is an im por tant de ter mi nant of in timal thic ke ning. He at shock pro te in 47 (HSP47), a col la gen-spe ci fic mo le cu lar cha pe ro ne, is tho ught to be es sen ti al for the pro ces sing and sec re ti on of pro col la gen mo le cu les. En dot he lin (ET) is a strong che mo at rac tant and mi to gen pro mo ting SMC pro li fe ra ti on and mig ra ti on. The aim of this study was to in ves ti ga te the pos sib le ro le of HSP47 and its re la ti on to col la gen synthe sis, and the ef fects of a non se lec ti ve ETA/ETB re cep tor an ta go nist, TAK-044 in col lar-in du ced early at he rosc le ro sis mo del. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : New Ze a land whi te rab bits we re di vi ded in to two gro ups. Both gro ups re ce ived ve hic le (0.9% NaCl 0.8 ml/kg/day, s.c.) or TAK-044 (5 mg/kg/day, s.c.) for three we eks. On 8 th day, a non-occ lu si ve si li con col lar was pla ced aro und the left ca ro tid ar tery. The right ca ro tid ar tery was sham-ope ra ted. HSP47 ex pres si on in ca ro tid ar te ri es we re de ter mi ned im mu no his toc he mi cally. Furt her mo re, to tal col la gen le vels, col la gen ex pres si on and type I pro col la gen ex pres si on we re es tab lished. R Re e s su ul lt ts s: : HSP47 ex pres si on cor re la ted with col la gen ex pres si on did not chan ge in col la red ar te ri es. TAK-044 tre at ment did not af fect HSP47 and col la gen le vels. C Co on nc c l lu u s si i o on n: : The re was a cor re la ti on bet we en HSP47 ex pres si on and col la gen ex pres si on in ca ro tid ar te ri es. Ho we ver, in ti mal thic ke ning did not in du ce HSP47 ex pres si on and early col la gen de ve lop ment. The inef fec ti ve nes of TAK-044 sug gests that ET-1 sig na ling may not be imp li ca ted in HSP47 and col la gen in this mo del. K Ke ey y W Wo or rd ds s: : HSP47 he at-shock pro te ins; at he rosc le ro sis; col la gen; en dot he lin-1; rab bits Ö ÖZ ZE ET T A Am ma aç ç: : Eks tra sel lü ler mat riks te ki (ESM) ko la jen ate rosk le roz ve res te no zun ge li şi mi sı ra sın -da vas kü ler ha sa ra ya nı tın mo dü las yo nun da önem li bir rol oy nar. Ko la jen düz kas hüc re si pro li feras yo nu nu, mig ras yo nu nu ve mat riks me tal lop ro te i naz (MMP) üre ti mi ni dü zen le ye bi lir. Bu ne den le ar ter ler de ki ko la jen dön gü sü in ti mal ka lın laş ma nın önem li bir be lir te ci dir. Ko la je ne öz gü mo le kü -ler bir şape ron olan ısı şoku pro te i ni 47'nin (HSP47) pro ko la jen mo le kül le ri nin iş len me si ve sek resyo nu için te mel ol du ğu dü şü nül mek te dir. En do t...

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Heat shock protein (HSP) 47 and collagen are upregulated during neointimal formation in the balloon-injured rat carotid artery

Cited by 29 publications

References 30 publications

Expression of HSP47, a collagen-specific chaperone, in normal and diseased human liver

Expression of HSP47, a collagen-specific chaperone, in normal and diseased human liver

The Kruppel-like Factor Zf9 and Proteins in the Sp1 Family Regulate the Expression of HSP47, a Collagen-specific Molecular Chaperone

Endothelin Receptor-Independent Correlation Between HSP47 and Collagen in Rabbit Model of Early Atherosclerosis

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