Heat Shock Protein (Hsp) Response Within RNA Viral Nervous Necrosis (VNN) that Infect of the Humpback grouper Cromileptes altivelis

Abstract

Abstract-Induction of Hsp can trigger by cellular stressorscaused by viral infections. The aim of this study is to find out of the expression of Hsp70 on C.altivelis exposured by infection of VNN. The methods were to isolate fractionate the protein of C.altivelis organs both normal and infected by of VNN by SDS-Page, and to detect the expression of Hsp70 qualitatively on tissue using Immunohistochemistry (IHC) methods. The result shows the IHC test was a positive Hsp70. The infection of VNN that have immunogenic protein on tissue organ of C.altivelis had been reacted by expression of function Hsp70. It means antigen determinant from the immunogenic protein of VNN has sensitivity highly on the reaction of Hsp70. Hsp70 can induce immune cell for the maintenance of Humpback grouper tissue receptors. The conclusion was the infection of VNN of C.altivelis was related to the expression of Hsp70.