Heat Shock Protein Reports on Proteome Stress

Liu, Yu; Zhang, Xin

doi:10.1002/biot.201800039

Cited by 6 publications

(5 citation statements)

References 12 publications

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“…These chaperones are responsible for folding, refolding, and protein remodeling, also acting in the transport and protection of these proteins. Still, in this context, the HSPs are also considered as markers of oxidative stress at the cellular level [ 54 ]. In view of this, we emphasize that the oxidative biochemistry imbalance in cerebellum of mice caused by exposure to fluoride was also able to modulate the proteomic profile.…”

Section: Discussionmentioning

confidence: 99%

Effects of Fluoride Long-Term Exposure over the Cerebellum: Global Proteomic Profile, Oxidative Biochemistry, Cell Density, and Motor Behavior Evaluation

Lopes

Ferreira

Davis

et al. 2020

IJMS

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Although the literature does not provide evidence of health risks from exposure to fluoride (F) in therapeutic doses, questions remain about the effects of long-term and high-dose use on the function of the central nervous system. The objective of this study was to investigate the effect of long-term exposure to F at levels similar to those found in areas of artificial water fluoridation and in areas of endemic fluorosis on biochemical, proteomic, cell density, and functional parameters associated with the cerebellum. For this, mice were exposed to water containing 10 mg F/L or 50 mg F/L (as sodium fluoride) for 60 days. After the exposure period, the animals were submitted to motor tests and the cerebellum was evaluated for fluoride levels, antioxidant capacity against peroxyl radicals (ACAP), lipid peroxidation (MDA), and nitrite levels (NO). The proteomic profile and morphological integrity were also evaluated. The results showed that the 10 mg F/L dose was able to decrease the ACAP levels, and the animals exposed to 50 mg F/L presented lower levels of ACAP and higher levels of MDA and NO. The cerebellar proteomic profile in both groups was modulated, highlighting proteins related to the antioxidant system, energy production, and cell death, however no neuronal density change in cerebellum was observed. Functionally, the horizontal exploratory activity of both exposed groups was impaired, while only the 50 mg F/L group showed significant changes in postural stability. No motor coordination and balance impairments were observed in both groups. Our results suggest that fluoride may impair the cerebellar oxidative biochemistry, which is associated with the proteomic modulation and, although no morphological impairment was observed, only the highest concentration of fluoride was able to impair some cerebellar motor functions.

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Section: Discussionmentioning

confidence: 99%

Effects of Fluoride Long-Term Exposure over the Cerebellum: Global Proteomic Profile, Oxidative Biochemistry, Cell Density, and Motor Behavior Evaluation

Lopes

Ferreira

Davis

et al. 2020

IJMS

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“…Besides heat stress (Baena et al, 2018; Sales et al, 2018), heat shock proteins (HSP) are being evaluated in apoptosis, oxidative stress, inflammatory diseases, cancer (Ikwegbue et al, 2017), virus infection (Shan et al, 2018), bacterial infection (Kim et al, 2018), as immunomodulators (Edkins et al, 2017; Zininga et al, 2018), therapeutics (Skórzynska-Dziduszko et al, 2018), and diagnostics (Lechner et al, 2018; Tang T. et al, 2018). HSPs have diagnostic role in ischemic injury of cardiomyocytes (Santos et al, 2018), inflammatory process of multiple sclerosis (Lechner et al, 2018), early diagnosis of lung cancer (Tang T. et al, 2018), proteome stress (Liu and Zhang, 2018), bipolar disorder (Cheng et al, 2018), and anti-apoptotic agent (Jang et al, 2018).…”

Section: Heat Shock Proteins (Hsps)mentioning

confidence: 99%

Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values

Dhama

Latheef

Dadar

et al. 2019

Front. Mol. Biosci.

220

172

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Various internal and external factors negatively affect the homeostatic equilibrium of organisms at the molecular to the whole-body level, inducing the so-called state of stress. Stress affects an organism's welfare status and induces energy-consuming mechanisms to combat the subsequent ill effects; thus, the individual may be immunocompromised, making them vulnerable to pathogens. The information presented here has been extensively reviewed, compiled, and analyzed from authenticated published resources available on Medline, PubMed, PubMed Central, Science Direct, and other scientific databases. Stress levels can be monitored by the quantitative and qualitative measurement of biomarkers. Potential markers of stress include thermal stress markers, such as heat shock proteins (HSPs), innate immune markers, such as Acute Phase Proteins (APPs), oxidative stress markers, and chemical secretions in the saliva and urine. In addition, stress biomarkers also play critical roles in the prognosis of stress-related diseases and disorders, and therapy guidance. Moreover, different components have been identified as potent mediators of cardiovascular, central nervous system, hepatic, and nephrological disorders, which can also be employed to evaluate these conditions precisely, but with stringent validation and specificity. Considerable scientific advances have been made in the detection, quantitation, and application of these biomarkers. The present review describes the current progress of identifying biomarkers, their prognostic, and therapeutic values.

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“…Among them, three strains ( i.e. , BL21, T7, and C43) were used to explore the optimal T7 RNA polymerase level to interact with the T7 promoter, 29 while BL21pLysS and BL21CodonPlus could improve the expression of toxic genes 30,31 and favor the translation of rare codons, respectively. In this study, similar amounts of soluble target proteins (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…28 To determine the most promising strain for efficient ALA production by the C5 pathway, five E. coli DE3 strains, i.e., BL21, T7, C43, BL21pLysS, and BL21CodonPlus, applicable to various conditions were tested. Among them, three strains (i.e., BL21, T7, and C43) were used to explore the optimal T7 RNA polymerase level to interact with the T7 promoter, 29 while BL21pLysS and BL21CodonPlus could improve the expression of toxic genes 30,31 and favor the translation of rare codons, respectively. In this study, similar amounts of soluble target proteins (Fig.…”

Section: Catalysis Science and Technology Papermentioning

confidence: 99%

Efficient biosynthesis of 5-aminolevulinic acid from glutamate via whole-cell biocatalyst in immobilized engineered Escherichia coli

Ying

Liu

Yang

et al. 2023

Catal. Sci. Technol.

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Heat Shock Protein Reports on Proteome Stress

Cited by 6 publications

References 12 publications

Effects of Fluoride Long-Term Exposure over the Cerebellum: Global Proteomic Profile, Oxidative Biochemistry, Cell Density, and Motor Behavior Evaluation

Effects of Fluoride Long-Term Exposure over the Cerebellum: Global Proteomic Profile, Oxidative Biochemistry, Cell Density, and Motor Behavior Evaluation

Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values

Efficient biosynthesis of 5-aminolevulinic acid from glutamate via whole-cell biocatalyst in immobilized engineered Escherichia coli

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