Heat Shock Proteins in Aquaculture Disease Immunology and Stress Response of Crustaceans

Li, Peng

doi:10.1007/978-3-319-73377-7_10

Cited by 6 publications

(3 citation statements)

References 219 publications

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“…Studies with onshore crab Carcinus maenas demonstrated that HSP70 in gill tissue did not reflect the degree of metal exposure in animals collected from field sites contaminated with metals [26]. In studies carried out with the mud crab estuary Scylla paramamosain, it was observed that the gills seem to be the primary and temporary target of metal accumulation, initiating the detoxification process, which will be completed in the hepatopancreas through the increased expression of HSP genes, which are related to apoptosis and the antioxidant system [11,27]. In addition, crabs Scylla paramamosain exposed to cadmium showed low expression of HSP genes in gills, being significantly expressed in hepatopancreas [11], corroborating the data found in our study.…”

Section: Discussionmentioning

confidence: 99%

HSP70 in Gills and Hepatopancreas of Mangrove Crabs Ucides cordatus: Comparison between Contaminated and Pristine Environments

Ortega

Vitorino

2022

Ecologies

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This study analyzed field and acclimatized (7 days) mangrove Ucides cordatus crabs from polluted and unpolluted environments to compare their HSP70 levels. The animals were cryo-anesthetized and dissected. Gills (anterior and posterior) and hepatopancreas were collected to evaluate total proteins and HSP70 levels using ELISA (Enzyme-Linked Immunosorbent Assay) method. The acclimatized animals from polluted environments showed higher HSP70 levels in the hepatopancreas than field animals. Results showed higher HSP70 levels in laboratory animals from the polluted environment than in field animals in the posterior gills. The regulation to decrease the damage caused by the environment and the acclimatization process may not be sufficient to stabilize physiological responses, especially in animals from polluted environments.

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Section: Discussionmentioning

confidence: 99%

HSP70 in Gills and Hepatopancreas of Mangrove Crabs Ucides cordatus: Comparison between Contaminated and Pristine Environments

Ortega

Vitorino

2022

Ecologies

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“…Heat shock proteins (HSP) play an important role in protecting organisms from almost any sudden change in the cellular environment that induces protein damage (Li, 2017), and their expression can take more time (>4 h) to reach the high expression levels under stress factors (Dennis III et al, 2015). Green crab (Carcinus maenas) under high CO 2 concentrations during more than seven days altered its HSP gene expression (Fehsenfeld et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional response of immune-related genes in Litopenaeus vannamei cultured in recirculating aquaculture systems with elevated CO2

Casillas-Hernández¹,

Arévalo‐Sainz²,

Flores‐Pérez³

et al. 2021

Revista Brasileira De Zootecnia

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This short-term study evaluated the effect of non-lethal high CO 2 concentration on the transcriptional response of immune-related genes of Pacific white shrimp (Litopenaeus vannamei) cultured in recirculating aquaculture systems (RAS). Two experimental groups were created: high CO 2 (47.67±2.04 mg L −1 ) and low CO 2 (2.0±1.93 mg L −1 ). Shrimp of 8.85±1.20 g were placed randomly at a density equivalent to 100 individuals m −3 and were monitored at 6, 12, 18, and 24 h. The transcriptional response of immune-related genes was analyzed by qPCR. Gene expression of hemocyanin, prophenoloxidase, and heat shock protein 60 was downregulated at 24 h, suggesting affectations on oxygen transportation, melanization, and protein functioning of L. vannamei under high CO 2 concentrations. Also, gene up-regulation of lipopolysaccharide-and β-glucan-binding protein and cytosolic manganese superoxide dismutase can impair the bacterial recognition and antioxidant defense of shrimp exposed to high CO 2 concentrations. These results suggest that concentration at about 47 mg L −1 of CO 2 can significantly influence the transcriptional response modulation of immune-related genes.

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“…Further analysis revealed that the majority of differentially expressed transcripts belonged to the Hsp20 family. Heat shock proteins are involved in a universal molecular stress response and play an important role for cell growth and viability (Jung and Lee, 2012;Li, 2017). Previous studies showed that Hsp20 is involved in cellular defence under environmental stress conditions.…”

Section: Legacy Contamination Near Storm Drains In Embayments Linked To Altered Bacterial Community Composition and Functioningmentioning

confidence: 99%

Legacy Metal Contaminants and Excess Nutrients in Low Flow Estuarine Embayments Alter Composition and Function of Benthic Bacterial Communities

et al. 2021

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Coastal systems such as estuaries are threatened by multiple anthropogenic stressors worldwide. However, how these stressors and estuarine hydrology shape benthic bacterial communities and their functions remains poorly known. Here, we surveyed sediment bacterial communities in poorly flushed embayments and well flushed channels in Sydney Harbour, Australia, using 16S rRNA gene sequencing. Sediment samples were collected monthly during the Austral summer-autumn 2014 at increasing distance from a large storm drain in each channel and embayment. Bacterial communities differed significantly between sites that varied in proximity to storm drains, with a gradient of change apparent for sites within embayments. We explored this pattern for embayment sites with analysis of RNA-Seq gene expression patterns and found higher expression of multiple genes involved in bacterial stress response far from storm drains, suggesting that bacterial communities close to storm drains may be more tolerant of localised anthropogenic stressors. Several bacterial groups also differed close to and far from storm drains, suggesting their potential utility as bioindicators to monitor contaminants in estuarine sediments. Overall, our study provides useful insights into changes in the composition and functioning of benthic bacterial communities as a result of multiple anthropogenic stressors in differing hydrological conditions.

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Heat Shock Proteins in Aquaculture Disease Immunology and Stress Response of Crustaceans

Cited by 6 publications

References 219 publications

HSP70 in Gills and Hepatopancreas of Mangrove Crabs Ucides cordatus: Comparison between Contaminated and Pristine Environments

HSP70 in Gills and Hepatopancreas of Mangrove Crabs Ucides cordatus: Comparison between Contaminated and Pristine Environments

Transcriptional response of immune-related genes in Litopenaeus vannamei cultured in recirculating aquaculture systems with elevated CO2

Legacy Metal Contaminants and Excess Nutrients in Low Flow Estuarine Embayments Alter Composition and Function of Benthic Bacterial Communities

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