Heat shock proteins in children and young adults on chronic hemodialysis

Musiał, Kinga; Szprynger, Krystyna; Szczepańska, Maria; Zwołińska, Danuta

doi:10.1007/s00467-009-1197-7

Cited by 15 publications

(10 citation statements)

References 34 publications

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“…There are no available data on human antiHsp60 in the patients on dialysis, except for our preliminary results showing the increased anti-Hsp60 concentrations in HD children and their significant decrease after a single HD session [16] . Lederer et al [17] reported on the increased titers of antibodies against mycobacterial Hsp65 in the HD subjects with vascular events, but van Roon et al [18] proved that the reactions against bacterial and human HSP differ from each other.…”

Section: Discussionmentioning

confidence: 71%

“…There are no data, apart from our preliminary results, on HSP in the patients with chronic kidney disease, regardless of age or on the differentiation between the dialysis modalities in relation to the HSP concentrations in such a population to date [16] . The aim of our study was to evaluate the levels of circulating Hsp60, Hsp90-␣ , anti-Hsp60 and anti-Hsp70 in the serum samples from children on peritoneal dialysis and hemodialysis (HD), and to analyze if there is any difference in the HSP profile between those groups corresponding to lipid disturbances, markers of endothelial activation (sE-selectin) or inflammation (hsCRP, IL-4, IL-12), and whether HSP may serve as markers of atherosclerosis in that group of patients.…”

mentioning

confidence: 57%

“…Unfortunately, data on Hsp90-␣ in dialyzed patients are scarce [16] . However, such elevation may support the assumption that the protective role of the elevated protein concentration acts as a danger signal [14] .…”

Section: Discussionmentioning

confidence: 99%

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The Impact of Dialysis Modality on Serum Heat Shock Proteins in Children and Young Adults with Chronic Kidney Disease

Musiał

Szczepańska²,

Szprynger³

et al. 2009

Kidney Blood Press Res

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Background/Aims: The acceleration of atherosclerosis by dialysis is not completely understood. The elevated levels of heat shock proteins (HSP) and antibodies against them were described in adults with atherosclerotic lesions, but there are no investigations on them in dialyzed patients. The aim of the studywas to evaluate the serum HSP, their connections with dialysis modality and their possible role as markers of atherosclerosis. Methods: Hsp60, Hsp90-α, anti-Hsp60 and anti-Hsp70 concentrations were assessed by ELISA in 19 children on automated peritoneal dialysis (APD) and 17 patients on hemodialysis (HD). hsCRP, sE-selectin, IL-4, IL-12 and the lipid profile were also estimated. Results: Hsp60 values were lower, whereas Hsp90-α was higher in the dialyzed children than in the controls, without any difference between the dialysis modalities. The anti-Hsp60 concentrations were increased in all patients and higher in HD patients than in APD patients. The anti-Hsp70 levels in the APD children were decreased versus controls. Anti-Hsp60 correlated with HDL-cholesterol in APD children, and with total cholesterol and LDL-cholesterol in HD patients. Anti-Hsp70 correlated with sE-selectin in HD subjects. Conclusions: The levels of antibodies against HSP seem to distinguish between the dialysis modalities and the correlations with the lipid profile and sE-selectin, suggesting their potential role as markers of atherosclerosis.

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Section: Discussionmentioning

confidence: 71%

mentioning

confidence: 57%

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The Impact of Dialysis Modality on Serum Heat Shock Proteins in Children and Young Adults with Chronic Kidney Disease

Musiał

Szczepańska²,

Szprynger³

et al. 2009

Kidney Blood Press Res

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“…In fact, while a strong correlation between anti-hHSP60 and atherosclerosis has been found in general population and in patients suffering from acute myocardial infarction (Heltai et al 2004), it has also been demonstrated that anti-hHSP60 antibodies are not associated to CVD events in diabetic and cardiopathic patients (Gruden et al 2009;Hoymans et al 2008). Regarding HD patients, high serum levels of anti-hHSP60 antibodies were reported in children and young HD patients, whereas adult subjects did not show different serum levels of antihHSP60 when compared to healthy control group (Musial et al 2009;Esposito et al 2010). Similar data resulted from the present study confirms our previous report.…”

Section: Discussionmentioning

confidence: 97%

Impact of seropositivity to Chlamydia pneumoniae and anti-hHSP60 on cardiovascular events in hemodialysis patients

Esposito

Tinelli

Libetta

et al. 2011

Cell Stress and Chaperones

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Autoimmunity to heat shock protein 60 (HSP60) has been related to atherosclerosis. Chlamydia pneumoniae (CP), the most studied infectious agent implicated in promoting atherosclerosis, produces a form of HSP60, which can induce an autoimmune response, due to high antigenic homology with human HSP60 (hHSP60). In this study, we evaluated the correlations among anti-hHSP60 antibodies, CP infection, and cardiovascular disease (CVD) in a high-risk population, such as patients undergoing hemodialysis (HD). Thirty-two patients (67.9±13.9 years; male/female, 23:9) on regular HD were enrolled. Global absolute cardiovascular risk (GCR) was assessed using the Italian CUORE Project's risk charts, which evaluate age, gender, smoking habits, diabetes, systolic blood pressure, and serum cholesterol. The occurrence of cardiovascular events during a 24-month follow-up was recorded. Seropositivity to CP and the presence of anti-hHSP60 antibodies were tested by specific enzyme-linked immunosorbent assays. Inflammation was assessed by measurement of Creactive protein (CRP) serum levels. Fifteen healthy sex and age-matched (61.9± 9.5 years; male/female, 11:4) subjects were the control group. Fifteen of 32 patients resulted seropositive for CP. CP+patients were older than CP−, while they did not differ for GCR, CRP, and dialytic parameters. CVD incidence was significantly higher in CP+ (9 CP+ vs 2 CP−, p<0.05). Cox analysis recognized that the incidence of CVD was independently correlated with seropositivity to CP (HR, 7.59; p=0.01;). On the other hand, there were no significant differences in anti-hHSP60 levels among CP+, CP− and healthy subjects: 18.11 μg/mL (14.8-47.8), 31.4 μg/mL (23.2-75.3), and 24.72 μg/mL (17.7-41.1), respectively. Anti-hHSP60 did not correlate to GCR, CRP, and incidence of CVD. In conclusion, our data suggest that anti-hHSP60 autoimmune response is not related to CP infection and CPrelated CVD risk in HD patients.

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“…For patients with other abnormalities, Lee et al reported that plasma from acute respiratory distress syndrome group had higher levels of GRP94, Hsp90, Hsp60, Hsp47, GPx-3, and interleukin-8 (Lee et al, 2012). Musial et al reported higher levels of Hsp90α in children with chronic kidney disease undergoing dialysis (Musial et al, 2009a) and children undergoing chronic hemodialysis (Musial et al, 2009b). Finally, Hacker et al reported that Hsp27, Hsp70 and Hsp90α were significantly altered in patients with chronic obstructive pulmonary disease, a leading cause of death characterized by increased cellular stress and inflammation (Hacker et al, 2009).…”

Section: Ehsp90 In Blood Circulation In Normal and Cancer Patientsmentioning

confidence: 99%

Extracellular Hsp90 (eHsp90) as the Actual Target in Clinical Trials

Li¹,

Tsen²,

Sahu³

et al. 2013

International Review of Cell and Molecular Biology

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Despite extensive investigative studies and clinical trials over the past two decades, we still do not understand why cancer cells are more sensitive to the cellular toxicity of Hsp90 inhibitors than normal cells. We still do not understand why only some cancer cells are sensitive to the Hsp90 inhibitors. Based on studies of the past few years, we argue that the selected sensitivity of cancer cells to Hsp90 inhibitors, such as 17-N-allylamino-17-demethoxygeldanamycin, is due to inhibition of the extracellular Hsp90 (eHsp90) rather than intracellular Hsp90 by these inhibitors. Because not all tumor cells utilize eHsp90 for motility, invasion and metastasis, only the group of “eHsp90-dependent” cancer cells is sensitive to Hsp90 inhibitors. If these notions prove to be true, pharmaceutical agents that selectively target eHsp90 should be more effective on tumor cells and less toxic on normal cells than current inhibitors that nondiscriminatively target both extracellular and intracellular Hsp90.

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Heat shock proteins in children and young adults on chronic hemodialysis

Cited by 15 publications

References 34 publications

The Impact of Dialysis Modality on Serum Heat Shock Proteins in Children and Young Adults with Chronic Kidney Disease

The Impact of Dialysis Modality on Serum Heat Shock Proteins in Children and Young Adults with Chronic Kidney Disease

Impact of seropositivity to Chlamydia pneumoniae and anti-hHSP60 on cardiovascular events in hemodialysis patients

Extracellular Hsp90 (eHsp90) as the Actual Target in Clinical Trials

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