Heat shock proteins in Daphnia detected using commercial antibodies: description and responsiveness to thermal stress

Mikulski, Andrzej; Grzesiuk, Małgorzata; Kloc, Małgorzata; Pijanowska, Joanna

doi:10.1007/s00049-009-0010-1

Cited by 14 publications

(13 citation statements)

References 25 publications

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“…Interestingly, previous work by Mikulski et al . [ 27 ] has shown that in response to thermal stress, a single clone of D. magna exhibited differential expression of isoforms of hsp70, with upregulation of the 73 and 74 kDa proteins, and inhibition of the 79 kDa protein. Whether such a response varies among genotypes/clones of Daphnia remains an open question.…”

Section: Discussionmentioning

confidence: 99%

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Resurrected ‘ancient’ Daphnia genotypes show reduced thermal stress tolerance compared to modern descendants

et al. 2018

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Understanding how populations adapt to rising temperatures has been a challenge in ecology. Research often evaluates multiple populations to test whether local adaptation to temperature regimes is occurring. Space-for-time substitutions are common, as temporal constraints limit our ability to observe evolutionary responses. We employed a resurrection ecology approach to understand how thermal tolerance has changed in a Daphnia pulicaria population over time. Temperatures experienced by the oldest genotypes were considerably lower than the youngest. We hypothesized clones were adapted to the thermal regimes of their respective time periods. We performed two thermal shock experiments that varied in length of heat exposure. Overall trends revealed that younger genotypes exhibited higher thermal tolerance than older genotypes; heat shock protein (hsp70) expression increased with temperature and varied among genotypes, but not across time periods. Our results indicate temperature may have been a selective factor on this population, although the observed responses may be a function of multifarious selection. Prior work found striking changes in population genetic structure, and in other traits that were strongly correlated with anthropogenic changes. Resurrection ecology approaches should help our understanding of interactive effects of anthropogenic alterations to temperature and other stressors on the evolutionary fate of natural populations.

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Section: Discussionmentioning

confidence: 99%

“…predation [ 26 ]) and abiotic (i.e. temperature [ 27 ]) stressors. These stressors have the potential for causing significant impacts (i.e.…”

Section: Introductionmentioning

confidence: 99%

Resurrected ‘ancient’ Daphnia genotypes show reduced thermal stress tolerance compared to modern descendants

et al. 2018

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“…To validate blotting and verify equal protein amounts in the lanes [ 18 , 75 ], proteins on the membrane were stained with 0.2% Ponceau S in 1% acetic acid. After blocking the membrane in saline phosphate buffer containing 5% dried milk, the blot was probed using primary anti-HSP60 (SPA-805, dilution 1:1000; Biotrend) as described in [ 76 ] and subsequently goat anti-rabbit horseradish peroxidase-conjugated secondary antibody solution (A6154, dilution, 1: 10000; Sigma Aldrich, Germany). Detection of the chemiluminescence upon incubation with luminol (final concentration, 1.25 mmol/L) and p-coumaric acid (final concentration, 0.2 mmol/L) was performed by exposure to a photographic film (Amersham Hyperfilm ECL, GE Healthcare, Germany).…”

Section: Methodsmentioning

confidence: 99%

The transcriptomic and proteomic responses of Daphnia pulex to changes in temperature and food supply comprise environment-specific and clone-specific elements

et al. 2018

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BackgroundRegulatory adjustments to acute and chronic temperature changes are highly important for aquatic ectotherms because temperature affects their metabolic rate as well as the already low oxygen concentration in water, which can upset their energy balance. This also applies to severe changes in food supply. Thus, we studied on a molecular level (transcriptomics and/or proteomics) the immediate responses to heat stress and starvation and the acclimation to different temperatures in two clonal isolates of the model microcrustacean Daphnia pulex from more or less stressful environments, which showed a higher (clone M) or lower (clone G) tolerance to heat and starvation.ResultsThe transcriptomic responses of clone G to acute heat stress (from 20 °C to 30 °C) and temperature acclimation (10 °C, 20 °C, and 24 °C) and the proteomic responses of both clones to acute heat, starvation, and heat-and-starvation stress comprised environment-specific and clone-specific elements. Acute stress (in particular heat stress) led to an early upregulation of stress genes and proteins (e.g., molecular chaperones) and a downregulation of metabolic genes and proteins (e.g., hydrolases). The transcriptomic responses to temperature acclimation differed clearly. They also varied depending on the temperature level. Acclimation to higher temperatures comprised an upregulation of metabolic genes and, in case of 24 °C acclimation, a downregulation of genes for translational processes and collagens. The proteomic responses of the clones M and G differed at any type of stress. Clone M showed markedly stronger and less stress-specific proteomic responses than clone G, which included the consistent expression of a specific heat shock protein (HSP60) and vitellogenin (VTG-SOD).ConclusionsThe expression changes under acute stress can be interpreted as a switch from standard products of gene expression to stress-specific products. The expression changes under temperature acclimation probably served for an increase in energy intake (via digestion) and, if necessary, a decrease in energy expenditures (e.g, for translational processes). The stronger and less stress-specific proteomic responses of clone M indicate a lower degree of cell damage and an active preservation of the energy balance, which allowed adequate proteomic responses under stress, including the initiation of resting egg production (VTG-SOD expression) as an emergency reaction.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4742-6) contains supplementary material, which is available to authorized users.

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“…Induction of HSP70 1A in Daphnia magna after the exposure to diclofenac and dimethyl sulfoxide was described by Haap et al ( 2008 ). Production of HSP 60, 70 and 90 kDa in those crustaceans was also observed during heat stress (Mikulski et al, 2009 ; 2011 ). Our study showed that the induction of HSP70 1A was evident in daphnids subjected to heat stress but which were not treated with ECT.…”

Section: Discussionmentioning

confidence: 91%

Protective effects of ectoine on heat-stressed Daphnia magna

2014

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Ectoine (ECT) is an amino acid produced and accumulated by halophilic bacteria in stressful conditions in order to prevent the loss of water from the cell. There is a lack of knowledge on the effects of ECT in heat-stressed aquatic animals. The purpose of our study was to determine the influence of ECT on Daphnia magna subjected to heat stress with two temperature gradients: 1 and 0.1 °C/min in the range of 23–42 °C. Time to immobilisation, survival during recovery, swimming performance, heart rate, thoracic limb movement and the levels of heat shock protein 70 kDa 1A (HSP70 1A), catalase (CAT) and nitric oxide species (NOx) were determined in ECT-exposed and unexposed daphnids; we showed protective effects of ECT on Daphnia magna subjected to heat stress. Time to immobilisation of daphnids exposed to ECT was longer when compared to the unexposed animals. Also, survival rate during the recovery of daphnids previously treated with ECT was higher. ECT significantly attenuated a rapid increase of mean swimming velocity which was elevated in the unexposed daphnids. Moreover, we observed elevation of thoracic limb movement and modulation of heart rate in ECT-exposed animals. HSP70 1A and CAT levels were reduced in the presence of ECT. On the other hand, NOx level was slightly elevated in both ECT-treated and unexposed daphnids, however slightly higher NOx level was found in ECT-treated animals. We conclude that the exposure to ectoine has thermoprotective effects on Daphnia magna, however their mechanisms are not associated with the induction of HSP70 1A.

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Heat shock proteins in Daphnia detected using commercial antibodies: description and responsiveness to thermal stress

Cited by 14 publications

References 25 publications

Resurrected ‘ancient’ Daphnia genotypes show reduced thermal stress tolerance compared to modern descendants

Resurrected ‘ancient’ Daphnia genotypes show reduced thermal stress tolerance compared to modern descendants

The transcriptomic and proteomic responses of Daphnia pulex to changes in temperature and food supply comprise environment-specific and clone-specific elements

Protective effects of ectoine on heat-stressed Daphnia magna

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